Experiment: WM_Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt manC and wcaI are separated by 2 nucleotides wcaI and gmm overlap by 4 nucleotides gmm and fcl overlap by 1 nucleotides
MCAODC_20575: manC - mannose-1-phosphate guanylyltransferase ManC, at 926,346 to 927,782
manC
MCAODC_20580: wcaI - colanic acid biosynthesis fucosyltransferase WcaI, at 927,785 to 929,008
wcaI
MCAODC_20585: gmm - GDP-mannose mannosyl hydrolase, at 929,005 to 929,487
gmm
MCAODC_20590: fcl - GDP-L-fucose synthase, at 929,487 to 930,452
fcl
Position (kb) 927
928
929
930 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 926.848 kb on + strand, within manC at 926.848 kb on + strand, within manC at 926.848 kb on + strand, within manC at 926.849 kb on - strand, within manC at 926.849 kb on - strand, within manC at 926.849 kb on - strand, within manC at 926.849 kb on - strand, within manC at 926.849 kb on - strand, within manC at 926.849 kb on - strand, within manC at 926.870 kb on + strand, within manC at 926.871 kb on - strand, within manC at 926.991 kb on - strand, within manC at 927.170 kb on + strand, within manC at 927.171 kb on - strand, within manC at 927.187 kb on + strand, within manC at 927.188 kb on - strand, within manC at 927.206 kb on - strand, within manC at 927.206 kb on - strand, within manC at 927.206 kb on - strand, within manC at 927.206 kb on - strand, within manC at 927.304 kb on + strand, within manC at 927.304 kb on + strand, within manC at 927.371 kb on - strand, within manC at 927.378 kb on - strand, within manC at 927.468 kb on - strand, within manC at 927.727 kb on - strand at 927.783 kb on - strand at 927.797 kb on - strand at 927.803 kb on + strand at 927.806 kb on - strand at 927.829 kb on + strand at 927.830 kb on - strand at 927.830 kb on - strand at 927.849 kb on + strand at 927.850 kb on - strand at 927.932 kb on + strand, within wcaI at 927.933 kb on - strand, within wcaI at 927.933 kb on - strand, within wcaI at 927.997 kb on + strand, within wcaI at 928.027 kb on + strand, within wcaI at 928.126 kb on - strand, within wcaI at 928.126 kb on - strand, within wcaI at 928.451 kb on - strand, within wcaI at 928.584 kb on - strand, within wcaI at 928.600 kb on + strand, within wcaI at 928.600 kb on + strand, within wcaI at 928.601 kb on - strand, within wcaI at 928.601 kb on - strand, within wcaI at 928.792 kb on - strand, within wcaI at 928.792 kb on - strand, within wcaI at 928.792 kb on - strand, within wcaI at 928.881 kb on + strand, within wcaI at 928.882 kb on - strand, within wcaI at 928.882 kb on - strand, within wcaI at 928.950 kb on + strand at 928.950 kb on + strand at 928.951 kb on - strand at 928.980 kb on + strand at 928.981 kb on - strand at 928.984 kb on + strand at 928.984 kb on + strand at 928.984 kb on + strand at 928.985 kb on - strand at 928.985 kb on - strand at 928.985 kb on - strand at 929.108 kb on + strand, within gmm at 929.180 kb on + strand, within gmm at 929.180 kb on + strand, within gmm at 929.181 kb on - strand, within gmm at 929.212 kb on - strand, within gmm at 929.527 kb on + strand at 929.527 kb on + strand at 929.528 kb on - strand at 929.657 kb on - strand, within fcl at 929.723 kb on + strand, within fcl at 929.804 kb on + strand, within fcl at 929.925 kb on - strand, within fcl at 929.954 kb on - strand, within fcl at 929.954 kb on - strand, within fcl at 929.981 kb on - strand, within fcl at 930.004 kb on + strand, within fcl
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas09 remove 926,848 + manC MCAODC_20575 0.35 -0.8 926,848 + manC MCAODC_20575 0.35 +0.2 926,848 + manC MCAODC_20575 0.35 -0.9 926,849 - manC MCAODC_20575 0.35 +0.6 926,849 - manC MCAODC_20575 0.35 -1.2 926,849 - manC MCAODC_20575 0.35 -0.7 926,849 - manC MCAODC_20575 0.35 -0.7 926,849 - manC MCAODC_20575 0.35 +0.6 926,849 - manC MCAODC_20575 0.35 -0.1 926,870 + manC MCAODC_20575 0.36 +0.2 926,871 - manC MCAODC_20575 0.37 -1.3 926,991 - manC MCAODC_20575 0.45 +0.4 927,170 + manC MCAODC_20575 0.57 -0.7 927,171 - manC MCAODC_20575 0.57 +0.9 927,187 + manC MCAODC_20575 0.59 +0.7 927,188 - manC MCAODC_20575 0.59 +0.9 927,206 - manC MCAODC_20575 0.60 -1.8 927,206 - manC MCAODC_20575 0.60 +0.7 927,206 - manC MCAODC_20575 0.60 +0.7 927,206 - manC MCAODC_20575 0.60 +0.5 927,304 + manC MCAODC_20575 0.67 +1.1 927,304 + manC MCAODC_20575 0.67 -1.0 927,371 - manC MCAODC_20575 0.71 +0.1 927,378 - manC MCAODC_20575 0.72 -0.4 927,468 - manC MCAODC_20575 0.78 +0.6 927,727 - +0.2 927,783 - +0.8 927,797 - -0.2 927,803 + +0.0 927,806 - +1.0 927,829 + -0.2 927,830 - -0.6 927,830 - +0.6 927,849 + -1.2 927,850 - +0.6 927,932 + wcaI MCAODC_20580 0.12 -1.2 927,933 - wcaI MCAODC_20580 0.12 +0.0 927,933 - wcaI MCAODC_20580 0.12 -0.3 927,997 + wcaI MCAODC_20580 0.17 +0.8 928,027 + wcaI MCAODC_20580 0.20 +0.3 928,126 - wcaI MCAODC_20580 0.28 -0.5 928,126 - wcaI MCAODC_20580 0.28 -0.8 928,451 - wcaI MCAODC_20580 0.54 +1.3 928,584 - wcaI MCAODC_20580 0.65 -3.5 928,600 + wcaI MCAODC_20580 0.67 -0.5 928,600 + wcaI MCAODC_20580 0.67 +0.9 928,601 - wcaI MCAODC_20580 0.67 +0.2 928,601 - wcaI MCAODC_20580 0.67 +0.2 928,792 - wcaI MCAODC_20580 0.82 +0.1 928,792 - wcaI MCAODC_20580 0.82 -0.0 928,792 - wcaI MCAODC_20580 0.82 -2.9 928,881 + wcaI MCAODC_20580 0.90 +0.2 928,882 - wcaI MCAODC_20580 0.90 -0.2 928,882 - wcaI MCAODC_20580 0.90 -0.3 928,950 + +0.0 928,950 + +0.9 928,951 - -1.1 928,980 + -0.1 928,981 - -0.3 928,984 + +0.6 928,984 + -0.8 928,984 + +0.4 928,985 - +1.0 928,985 - -1.4 928,985 - -1.2 929,108 + gmm MCAODC_20585 0.21 -0.2 929,180 + gmm MCAODC_20585 0.36 +1.2 929,180 + gmm MCAODC_20585 0.36 -0.7 929,181 - gmm MCAODC_20585 0.36 +0.2 929,212 - gmm MCAODC_20585 0.43 +0.7 929,527 + +0.2 929,527 + -0.3 929,528 - +1.1 929,657 - fcl MCAODC_20590 0.18 -0.3 929,723 + fcl MCAODC_20590 0.24 +1.6 929,804 + fcl MCAODC_20590 0.33 +0.2 929,925 - fcl MCAODC_20590 0.45 +0.6 929,954 - fcl MCAODC_20590 0.48 -1.0 929,954 - fcl MCAODC_20590 0.48 -0.2 929,981 - fcl MCAODC_20590 0.51 +0.2 930,004 + fcl MCAODC_20590 0.54 -1.5
Or see this region's nucleotide sequence