Experiment: WM_Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hrpA and MCAODC_16210 are separated by 145 nucleotides MCAODC_16210 and MCAODC_16215 are separated by 4 nucleotides MCAODC_16215 and MCAODC_16220 overlap by 14 nucleotides MCAODC_16220 and MCAODC_16225 are separated by 124 nucleotides
MCAODC_16205: hrpA - ATP-dependent RNA helicase HrpA, at 136,454 to 140,356
hrpA
MCAODC_16210: MCAODC_16210 - hypothetical protein, at 140,502 to 140,927
_16210
MCAODC_16215: MCAODC_16215 - hypothetical protein, at 140,932 to 141,270
_16215
MCAODC_16220: MCAODC_16220 - hypothetical protein, at 141,257 to 141,688
_16220
MCAODC_16225: MCAODC_16225 - hypothetical protein, at 141,813 to 142,190
_16225
Position (kb) 140
141
142 Strain fitness (log2 ratio) -2
-1
0
1
2 at 139.979 kb on - strand at 140.031 kb on + strand at 140.032 kb on - strand at 140.323 kb on + strand at 140.324 kb on - strand at 140.399 kb on - strand at 140.458 kb on - strand at 141.797 kb on + strand at 141.947 kb on - strand, within MCAODC_16225 at 142.001 kb on + strand, within MCAODC_16225 at 142.001 kb on + strand, within MCAODC_16225 at 142.076 kb on + strand, within MCAODC_16225 at 142.077 kb on - strand, within MCAODC_16225 at 142.119 kb on + strand, within MCAODC_16225 at 142.178 kb on - strand at 142.182 kb on + strand at 142.219 kb on + strand at 142.262 kb on + strand at 142.265 kb on + strand at 142.266 kb on - strand at 142.266 kb on - strand at 142.266 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas09 remove 139,979 - -0.8 140,031 + +1.0 140,032 - -2.2 140,323 + +0.8 140,324 - -1.8 140,399 - +0.8 140,458 - -0.1 141,797 + +2.0 141,947 - MCAODC_16225 0.35 -0.8 142,001 + MCAODC_16225 0.50 +0.6 142,001 + MCAODC_16225 0.50 -1.7 142,076 + MCAODC_16225 0.70 +0.4 142,077 - MCAODC_16225 0.70 +0.3 142,119 + MCAODC_16225 0.81 -1.1 142,178 - +0.3 142,182 + +1.3 142,219 + -1.7 142,262 + -0.4 142,265 + +0.4 142,266 - -2.0 142,266 - -0.1 142,266 - -0.3
Or see this region's nucleotide sequence