Experiment: WM_Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pspC and pspD are separated by 8 nucleotides pspD and pspE are separated by 74 nucleotides pspE and MCAODC_15885 are separated by 209 nucleotides MCAODC_15885 and ycjM overlap by 1 nucleotides
MCAODC_15870: pspC - envelope stress response membrane protein PspC, at 66,832 to 67,191
pspC
MCAODC_15875: pspD - phage shock protein PspD, at 67,200 to 67,421
pspD
MCAODC_15880: pspE - thiosulfate sulfurtransferase PspE, at 67,496 to 67,810
pspE
MCAODC_15885: MCAODC_15885 - Alpha-amylase, at 68,020 to 68,394
_15885
MCAODC_15890: ycjM - Glucosylglycerate phosphorylase, at 68,394 to 69,698
ycjM
Position (kb) 68
69 Strain fitness (log2 ratio) -2
-1
0
1
2 at 67.141 kb on + strand, within pspC at 67.141 kb on + strand, within pspC at 67.142 kb on - strand, within pspC at 67.379 kb on - strand, within pspD at 67.456 kb on + strand at 67.459 kb on - strand at 67.481 kb on + strand at 67.622 kb on - strand, within pspE at 67.692 kb on - strand, within pspE at 67.692 kb on - strand, within pspE at 67.750 kb on + strand, within pspE at 67.750 kb on + strand, within pspE at 67.866 kb on - strand at 67.876 kb on - strand at 67.880 kb on + strand at 67.881 kb on - strand at 68.071 kb on - strand, within MCAODC_15885 at 68.691 kb on + strand, within ycjM at 68.823 kb on - strand, within ycjM at 68.891 kb on + strand, within ycjM at 69.167 kb on - strand, within ycjM at 69.283 kb on - strand, within ycjM
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas09 remove 67,141 + pspC MCAODC_15870 0.86 +0.7 67,141 + pspC MCAODC_15870 0.86 +1.5 67,142 - pspC MCAODC_15870 0.86 -0.3 67,379 - pspD MCAODC_15875 0.81 +0.6 67,456 + +0.1 67,459 - -0.8 67,481 + -0.5 67,622 - pspE MCAODC_15880 0.40 +1.0 67,692 - pspE MCAODC_15880 0.62 -0.8 67,692 - pspE MCAODC_15880 0.62 +0.7 67,750 + pspE MCAODC_15880 0.81 -1.8 67,750 + pspE MCAODC_15880 0.81 -0.0 67,866 - -0.4 67,876 - -1.6 67,880 + +0.2 67,881 - -0.1 68,071 - MCAODC_15885 0.14 +1.3 68,691 + ycjM MCAODC_15890 0.23 +0.8 68,823 - ycjM MCAODC_15890 0.33 +0.6 68,891 + ycjM MCAODC_15890 0.38 -0.0 69,167 - ycjM MCAODC_15890 0.59 +1.9 69,283 - ycjM MCAODC_15890 0.68 -0.4
Or see this region's nucleotide sequence