Strain Fitness in Escherichia coli ECRC101 around MCAODC_10035

Experiment: WM_Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntubiH and ubiI are separated by 23 nucleotidesubiI and gcvT are separated by 447 nucleotides MCAODC_10030: ubiH - 2-octaprenyl-6-methoxyphenyl hydroxylase, at 1,994,150 to 1,995,328 ubiH MCAODC_10035: ubiI - FAD-dependent 2-octaprenylphenol hydroxylase, at 1,995,352 to 1,996,554 ubiI MCAODC_10040: gcvT - glycine cleavage system aminomethyltransferase GcvT, at 1,997,002 to 1,998,096 gcvT Position (kb) 1995 1996 1997Strain fitness (log2 ratio) -1 0 1 2 3at 1995.339 kb on + strandat 1995.340 kb on - strandat 1996.575 kb on - strandat 1996.767 kb on + strandat 1996.830 kb on + strandat 1996.835 kb on + strandat 1996.853 kb on + strandat 1996.865 kb on - strandat 1996.884 kb on - strandat 1996.953 kb on - strandat 1997.023 kb on + strandat 1997.023 kb on + strandat 1997.089 kb on + strandat 1997.120 kb on + strand, within gcvTat 1997.120 kb on + strand, within gcvTat 1997.121 kb on - strand, within gcvTat 1997.121 kb on - strand, within gcvTat 1997.121 kb on - strand, within gcvTat 1997.121 kb on - strand, within gcvTat 1997.121 kb on - strand, within gcvTat 1997.121 kb on - strand, within gcvTat 1997.121 kb on - strand, within gcvTat 1997.121 kb on - strand, within gcvTat 1997.121 kb on - strand, within gcvTat 1997.154 kb on + strand, within gcvTat 1997.154 kb on + strand, within gcvTat 1997.154 kb on + strand, within gcvTat 1997.154 kb on + strand, within gcvTat 1997.154 kb on + strand, within gcvTat 1997.155 kb on - strand, within gcvTat 1997.155 kb on - strand, within gcvTat 1997.155 kb on - strand, within gcvTat 1997.155 kb on - strand, within gcvTat 1997.155 kb on - strand, within gcvTat 1997.155 kb on - strand, within gcvTat 1997.255 kb on - strand, within gcvTat 1997.289 kb on - strand, within gcvTat 1997.306 kb on - strand, within gcvTat 1997.309 kb on - strand, within gcvTat 1997.309 kb on - strand, within gcvTat 1997.341 kb on - strand, within gcvTat 1997.341 kb on - strand, within gcvTat 1997.441 kb on + strand, within gcvTat 1997.442 kb on - strand, within gcvTat 1997.481 kb on + strand, within gcvT

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas09
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1,995,339 + +1.7
1,995,340 - +0.2
1,996,575 - +0.4
1,996,767 + +0.1
1,996,830 + -0.5
1,996,835 + +0.2
1,996,853 + -1.3
1,996,865 - -0.1
1,996,884 - -1.4
1,996,953 - +0.2
1,997,023 + -0.5
1,997,023 + +1.8
1,997,089 + +0.1
1,997,120 + gcvT MCAODC_10040 0.11 +0.1
1,997,120 + gcvT MCAODC_10040 0.11 -1.5
1,997,121 - gcvT MCAODC_10040 0.11 -1.7
1,997,121 - gcvT MCAODC_10040 0.11 +0.7
1,997,121 - gcvT MCAODC_10040 0.11 -0.7
1,997,121 - gcvT MCAODC_10040 0.11 +0.2
1,997,121 - gcvT MCAODC_10040 0.11 -0.4
1,997,121 - gcvT MCAODC_10040 0.11 -0.0
1,997,121 - gcvT MCAODC_10040 0.11 +1.5
1,997,121 - gcvT MCAODC_10040 0.11 -1.2
1,997,121 - gcvT MCAODC_10040 0.11 -0.3
1,997,154 + gcvT MCAODC_10040 0.14 +3.0
1,997,154 + gcvT MCAODC_10040 0.14 -0.8
1,997,154 + gcvT MCAODC_10040 0.14 -0.1
1,997,154 + gcvT MCAODC_10040 0.14 +0.2
1,997,154 + gcvT MCAODC_10040 0.14 +1.3
1,997,155 - gcvT MCAODC_10040 0.14 -0.6
1,997,155 - gcvT MCAODC_10040 0.14 -0.9
1,997,155 - gcvT MCAODC_10040 0.14 -0.5
1,997,155 - gcvT MCAODC_10040 0.14 -0.5
1,997,155 - gcvT MCAODC_10040 0.14 +0.6
1,997,155 - gcvT MCAODC_10040 0.14 +0.2
1,997,255 - gcvT MCAODC_10040 0.23 -0.9
1,997,289 - gcvT MCAODC_10040 0.26 -0.5
1,997,306 - gcvT MCAODC_10040 0.28 -0.9
1,997,309 - gcvT MCAODC_10040 0.28 +1.4
1,997,309 - gcvT MCAODC_10040 0.28 +0.7
1,997,341 - gcvT MCAODC_10040 0.31 -0.6
1,997,341 - gcvT MCAODC_10040 0.31 +0.6
1,997,441 + gcvT MCAODC_10040 0.40 +0.1
1,997,442 - gcvT MCAODC_10040 0.40 +0.7
1,997,481 + gcvT MCAODC_10040 0.44 +0.2

Or see this region's nucleotide sequence