Experiment: R2A with 6-methoxy-2(3H)-benzoxazolone 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR88_RS02770 and ABZR88_RS02775 are separated by 85 nucleotides ABZR88_RS02775 and ABZR88_RS02780 overlap by 11 nucleotides ABZR88_RS02780 and ABZR88_RS02785 are separated by 53 nucleotides ABZR88_RS02785 and ABZR88_RS02790 are separated by 92 nucleotides
ABZR88_RS02770: ABZR88_RS02770 - LexA family transcriptional regulator, at 651,145 to 651,924
_RS02770
ABZR88_RS02775: ABZR88_RS02775 - nuclease, at 652,010 to 652,369
_RS02775
ABZR88_RS02780: ABZR88_RS02780 - peptide-methionine (R)-S-oxide reductase MsrB, at 652,359 to 652,844
_RS02780
ABZR88_RS02785: ABZR88_RS02785 - fasciclin domain-containing protein, at 652,898 to 653,482
_RS02785
ABZR88_RS02790: ABZR88_RS02790 - PD40 domain-containing protein, at 653,575 to 656,784
_RS02790
Position (kb)
652
653 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 651.619 kb on - strand, within ABZR88_RS02770 at 651.738 kb on + strand, within ABZR88_RS02770 at 652.025 kb on + strand at 652.032 kb on + strand at 652.052 kb on - strand, within ABZR88_RS02775 at 652.083 kb on + strand, within ABZR88_RS02775 at 652.210 kb on - strand, within ABZR88_RS02775 at 652.288 kb on - strand, within ABZR88_RS02775 at 652.368 kb on - strand at 652.589 kb on + strand, within ABZR88_RS02780 at 652.621 kb on + strand, within ABZR88_RS02780 at 652.697 kb on - strand, within ABZR88_RS02780 at 652.797 kb on + strand at 652.825 kb on - strand at 652.844 kb on + strand at 652.906 kb on + strand at 653.012 kb on + strand, within ABZR88_RS02785 at 653.039 kb on - strand, within ABZR88_RS02785 at 653.066 kb on - strand, within ABZR88_RS02785 at 653.068 kb on + strand, within ABZR88_RS02785 at 653.068 kb on + strand, within ABZR88_RS02785 at 653.149 kb on + strand, within ABZR88_RS02785 at 653.150 kb on - strand, within ABZR88_RS02785 at 653.296 kb on + strand, within ABZR88_RS02785 at 653.319 kb on + strand, within ABZR88_RS02785 at 653.482 kb on + strand at 653.483 kb on - strand at 653.494 kb on + strand at 653.662 kb on - strand at 653.723 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-methoxy-2(3H)-benzoxazolone 0.5 mM remove 651,619 - ABZR88_RS02770 0.61 -1.1 651,738 + ABZR88_RS02770 0.76 -0.1 652,025 + +0.6 652,032 + -1.0 652,052 - ABZR88_RS02775 0.12 +0.4 652,083 + ABZR88_RS02775 0.20 -0.5 652,210 - ABZR88_RS02775 0.56 +2.5 652,288 - ABZR88_RS02775 0.77 +0.3 652,368 - -0.4 652,589 + ABZR88_RS02780 0.47 -0.4 652,621 + ABZR88_RS02780 0.54 -0.4 652,697 - ABZR88_RS02780 0.70 -0.4 652,797 + -0.2 652,825 - -0.3 652,844 + -0.4 652,906 + -0.1 653,012 + ABZR88_RS02785 0.19 +1.1 653,039 - ABZR88_RS02785 0.24 -0.2 653,066 - ABZR88_RS02785 0.29 +0.0 653,068 + ABZR88_RS02785 0.29 -1.0 653,068 + ABZR88_RS02785 0.29 -0.8 653,149 + ABZR88_RS02785 0.43 -1.0 653,150 - ABZR88_RS02785 0.43 +0.5 653,296 + ABZR88_RS02785 0.68 -1.3 653,319 + ABZR88_RS02785 0.72 -0.9 653,482 + -1.4 653,483 - +1.6 653,494 + -1.2 653,662 - -1.5 653,723 + -2.5
Or see this region's nucleotide sequence