Experiment: R2A with 6-methoxy-2(3H)-benzoxazolone 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR88_RS02055 and ABZR88_RS02060 are separated by 345 nucleotides ABZR88_RS02060 and ABZR88_RS02065 are separated by 2 nucleotides ABZR88_RS02065 and ABZR88_RS02070 are separated by 3 nucleotides
ABZR88_RS02055: ABZR88_RS02055 - signal peptidase I, at 461,325 to 462,776
_RS02055
ABZR88_RS02060: ABZR88_RS02060 - undecaprenyl-diphosphate phosphatase, at 463,122 to 463,928
_RS02060
ABZR88_RS02065: ABZR88_RS02065 - DUF3098 domain-containing protein, at 463,931 to 464,200
_RS02065
ABZR88_RS02070: ABZR88_RS02070 - ABC transporter permease, at 464,204 to 465,082
_RS02070
Position (kb) 463
464 Strain fitness (log2 ratio) -1
0
1 at 462.186 kb on + strand, within ABZR88_RS02055 at 462.191 kb on + strand, within ABZR88_RS02055 at 462.193 kb on + strand, within ABZR88_RS02055 at 462.372 kb on + strand, within ABZR88_RS02055 at 462.420 kb on - strand, within ABZR88_RS02055 at 462.424 kb on - strand, within ABZR88_RS02055 at 462.518 kb on + strand, within ABZR88_RS02055 at 462.526 kb on + strand, within ABZR88_RS02055 at 462.556 kb on + strand, within ABZR88_RS02055 at 462.580 kb on - strand, within ABZR88_RS02055 at 462.597 kb on + strand, within ABZR88_RS02055 at 462.601 kb on - strand, within ABZR88_RS02055 at 462.676 kb on + strand at 462.736 kb on + strand at 462.784 kb on + strand at 462.845 kb on + strand at 463.096 kb on - strand at 463.140 kb on + strand at 463.140 kb on + strand at 464.212 kb on - strand at 464.214 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-methoxy-2(3H)-benzoxazolone 0.5 mM remove 462,186 + ABZR88_RS02055 0.59 +0.5 462,191 + ABZR88_RS02055 0.60 +0.2 462,193 + ABZR88_RS02055 0.60 +0.9 462,372 + ABZR88_RS02055 0.72 +0.9 462,420 - ABZR88_RS02055 0.75 +0.7 462,424 - ABZR88_RS02055 0.76 -0.6 462,518 + ABZR88_RS02055 0.82 +0.2 462,526 + ABZR88_RS02055 0.83 +0.6 462,556 + ABZR88_RS02055 0.85 +1.0 462,580 - ABZR88_RS02055 0.86 +0.3 462,597 + ABZR88_RS02055 0.88 +0.5 462,601 - ABZR88_RS02055 0.88 +0.2 462,676 + +0.2 462,736 + +1.4 462,784 + -0.2 462,845 + +0.3 463,096 - -0.7 463,140 + -0.3 463,140 + +0.2 464,212 - +0.3 464,214 - +0.5
Or see this region's nucleotide sequence