Experiment: TSB with Dermcidin 2 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX26_02010 and MPMX26_02011 are separated by 34 nucleotides MPMX26_02011 and MPMX26_02012 are separated by 45 nucleotides MPMX26_02012 and MPMX26_02013 are separated by 310 nucleotides
MPMX26_02010: MPMX26_02010 - hypothetical protein, at 2,076,009 to 2,076,215
_02010
MPMX26_02011: MPMX26_02011 - tRNA 5-hydroxyuridine methyltransferase, at 2,076,250 to 2,076,834
_02011
MPMX26_02012: MPMX26_02012 - hypothetical protein, at 2,076,880 to 2,077,095
_02012
MPMX26_02013: MPMX26_02013 - Alkyl hydroperoxide reductase subunit F, at 2,077,406 to 2,078,971
_02013
Position (kb)
2076
2077
2078 Strain fitness (log2 ratio)
-1
0
1
2 at 2076.271 kb on - strand at 2076.471 kb on + strand, within MPMX26_02011 at 2076.928 kb on - strand, within MPMX26_02012 at 2076.987 kb on - strand, within MPMX26_02012 at 2077.069 kb on - strand, within MPMX26_02012 at 2077.069 kb on - strand, within MPMX26_02012 at 2077.603 kb on + strand, within MPMX26_02013 at 2077.834 kb on + strand, within MPMX26_02013 at 2077.834 kb on + strand, within MPMX26_02013 at 2077.834 kb on + strand, within MPMX26_02013 at 2078.057 kb on + strand, within MPMX26_02013
Per-strain Table
Position Strand Gene LocusTag Fraction TSB with Dermcidin 2 ug/mL remove 2,076,271 - +0.2 2,076,471 + MPMX26_02011 0.38 -1.0 2,076,928 - MPMX26_02012 0.22 +0.4 2,076,987 - MPMX26_02012 0.50 +0.4 2,077,069 - MPMX26_02012 0.88 +1.8 2,077,069 - MPMX26_02012 0.88 +0.5 2,077,603 + MPMX26_02013 0.13 +0.9 2,077,834 + MPMX26_02013 0.27 +0.3 2,077,834 + MPMX26_02013 0.27 +0.7 2,077,834 + MPMX26_02013 0.27 +1.0 2,078,057 + MPMX26_02013 0.42 +0.9
Or see this region's nucleotide sequence