Experiment: TSB with Dermcidin 2 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX26_02768 and MPMX26_02769 are separated by 109 nucleotides MPMX26_02769 and MPMX26_02770 are separated by 74 nucleotides MPMX26_02770 and MPMX26_02771 are separated by 408 nucleotides MPMX26_02771 and MPMX26_02772 are separated by 78 nucleotides
MPMX26_02768: MPMX26_02768 - Putative transport protein YdiK, at 2,851,646 to 2,852,764
_02768
MPMX26_02769: MPMX26_02769 - Superoxide dismutase [Cu-Zn], at 2,852,874 to 2,853,422
_02769
MPMX26_02770: MPMX26_02770 - Chloramphenicol resistance protein CraA, at 2,853,497 to 2,854,726
_02770
MPMX26_02771: MPMX26_02771 - hypothetical protein, at 2,855,135 to 2,855,500
_02771
MPMX26_02772: MPMX26_02772 - putative FAD-linked oxidoreductase, at 2,855,579 to 2,856,988
_02772
Position (kb)
2853
2854
2855 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2852.585 kb on + strand, within MPMX26_02768 at 2852.585 kb on + strand, within MPMX26_02768 at 2852.590 kb on + strand, within MPMX26_02768 at 2852.591 kb on + strand, within MPMX26_02768 at 2852.604 kb on + strand, within MPMX26_02768 at 2852.618 kb on + strand, within MPMX26_02768 at 2852.631 kb on - strand, within MPMX26_02768 at 2852.692 kb on + strand at 2852.714 kb on + strand at 2852.786 kb on + strand at 2852.814 kb on + strand at 2852.819 kb on + strand at 2852.819 kb on + strand at 2852.835 kb on + strand at 2852.835 kb on + strand at 2852.835 kb on + strand at 2852.840 kb on + strand at 2852.933 kb on + strand, within MPMX26_02769 at 2852.943 kb on + strand, within MPMX26_02769 at 2852.943 kb on + strand, within MPMX26_02769 at 2852.970 kb on + strand, within MPMX26_02769 at 2852.970 kb on + strand, within MPMX26_02769 at 2852.970 kb on + strand, within MPMX26_02769 at 2852.970 kb on + strand, within MPMX26_02769 at 2852.970 kb on + strand, within MPMX26_02769 at 2852.972 kb on + strand, within MPMX26_02769 at 2852.985 kb on + strand, within MPMX26_02769 at 2852.999 kb on + strand, within MPMX26_02769 at 2853.006 kb on + strand, within MPMX26_02769 at 2853.006 kb on + strand, within MPMX26_02769 at 2853.046 kb on + strand, within MPMX26_02769 at 2853.046 kb on + strand, within MPMX26_02769 at 2853.048 kb on + strand, within MPMX26_02769 at 2853.048 kb on + strand, within MPMX26_02769 at 2853.048 kb on + strand, within MPMX26_02769 at 2853.048 kb on + strand, within MPMX26_02769 at 2853.048 kb on + strand, within MPMX26_02769 at 2853.055 kb on + strand, within MPMX26_02769 at 2853.055 kb on + strand, within MPMX26_02769 at 2853.055 kb on + strand, within MPMX26_02769 at 2853.055 kb on + strand, within MPMX26_02769 at 2853.077 kb on + strand, within MPMX26_02769 at 2853.081 kb on + strand, within MPMX26_02769 at 2853.086 kb on + strand, within MPMX26_02769 at 2853.086 kb on + strand, within MPMX26_02769 at 2853.086 kb on + strand, within MPMX26_02769 at 2853.086 kb on + strand, within MPMX26_02769 at 2853.086 kb on + strand, within MPMX26_02769 at 2853.086 kb on + strand, within MPMX26_02769 at 2853.086 kb on + strand, within MPMX26_02769 at 2853.093 kb on + strand, within MPMX26_02769 at 2853.103 kb on + strand, within MPMX26_02769 at 2853.198 kb on + strand, within MPMX26_02769 at 2853.198 kb on + strand, within MPMX26_02769 at 2853.198 kb on + strand, within MPMX26_02769 at 2853.198 kb on + strand, within MPMX26_02769 at 2853.204 kb on + strand, within MPMX26_02769 at 2853.204 kb on + strand, within MPMX26_02769 at 2853.204 kb on + strand, within MPMX26_02769 at 2853.204 kb on + strand, within MPMX26_02769 at 2853.204 kb on + strand, within MPMX26_02769 at 2853.204 kb on + strand, within MPMX26_02769 at 2853.204 kb on + strand, within MPMX26_02769 at 2853.204 kb on + strand, within MPMX26_02769 at 2853.204 kb on + strand, within MPMX26_02769 at 2853.204 kb on + strand, within MPMX26_02769 at 2853.204 kb on + strand, within MPMX26_02769 at 2853.204 kb on + strand, within MPMX26_02769 at 2853.213 kb on + strand, within MPMX26_02769 at 2853.213 kb on + strand, within MPMX26_02769 at 2853.223 kb on + strand, within MPMX26_02769 at 2853.276 kb on + strand, within MPMX26_02769 at 2853.276 kb on + strand, within MPMX26_02769 at 2853.276 kb on + strand, within MPMX26_02769 at 2853.286 kb on + strand, within MPMX26_02769 at 2853.297 kb on + strand, within MPMX26_02769 at 2853.297 kb on + strand, within MPMX26_02769 at 2853.314 kb on + strand, within MPMX26_02769 at 2853.324 kb on + strand, within MPMX26_02769 at 2853.335 kb on + strand, within MPMX26_02769 at 2853.353 kb on + strand, within MPMX26_02769 at 2853.358 kb on + strand, within MPMX26_02769 at 2853.372 kb on + strand at 2853.385 kb on + strand at 2853.385 kb on + strand at 2853.388 kb on + strand at 2853.566 kb on - strand at 2853.585 kb on - strand at 2853.585 kb on - strand at 2853.585 kb on - strand at 2853.585 kb on - strand at 2853.585 kb on - strand at 2853.590 kb on - strand at 2853.662 kb on - strand, within MPMX26_02770 at 2853.679 kb on - strand, within MPMX26_02770 at 2853.724 kb on - strand, within MPMX26_02770 at 2853.724 kb on - strand, within MPMX26_02770 at 2853.724 kb on - strand, within MPMX26_02770 at 2853.724 kb on - strand, within MPMX26_02770 at 2853.744 kb on - strand, within MPMX26_02770 at 2853.744 kb on - strand, within MPMX26_02770 at 2853.754 kb on - strand, within MPMX26_02770 at 2853.765 kb on - strand, within MPMX26_02770 at 2853.765 kb on - strand, within MPMX26_02770 at 2853.765 kb on - strand, within MPMX26_02770 at 2853.765 kb on - strand, within MPMX26_02770 at 2853.775 kb on - strand, within MPMX26_02770 at 2853.775 kb on - strand, within MPMX26_02770 at 2853.775 kb on - strand, within MPMX26_02770 at 2853.775 kb on - strand, within MPMX26_02770 at 2853.818 kb on - strand, within MPMX26_02770 at 2853.818 kb on - strand, within MPMX26_02770 at 2853.818 kb on - strand, within MPMX26_02770 at 2853.830 kb on - strand, within MPMX26_02770 at 2853.865 kb on - strand, within MPMX26_02770 at 2853.911 kb on - strand, within MPMX26_02770 at 2853.911 kb on - strand, within MPMX26_02770 at 2853.926 kb on - strand, within MPMX26_02770 at 2853.926 kb on - strand, within MPMX26_02770 at 2853.936 kb on - strand, within MPMX26_02770 at 2854.013 kb on - strand, within MPMX26_02770 at 2854.013 kb on - strand, within MPMX26_02770 at 2854.013 kb on - strand, within MPMX26_02770 at 2854.013 kb on - strand, within MPMX26_02770 at 2854.013 kb on - strand, within MPMX26_02770 at 2854.013 kb on - strand, within MPMX26_02770 at 2854.013 kb on - strand, within MPMX26_02770 at 2854.013 kb on - strand, within MPMX26_02770 at 2854.013 kb on - strand, within MPMX26_02770 at 2854.036 kb on - strand, within MPMX26_02770 at 2854.129 kb on - strand, within MPMX26_02770 at 2854.206 kb on - strand, within MPMX26_02770 at 2854.223 kb on - strand, within MPMX26_02770 at 2854.280 kb on - strand, within MPMX26_02770 at 2854.300 kb on - strand, within MPMX26_02770 at 2854.335 kb on - strand, within MPMX26_02770 at 2854.335 kb on - strand, within MPMX26_02770 at 2854.370 kb on - strand, within MPMX26_02770 at 2854.386 kb on - strand, within MPMX26_02770 at 2854.388 kb on - strand, within MPMX26_02770 at 2854.468 kb on - strand, within MPMX26_02770 at 2854.468 kb on - strand, within MPMX26_02770 at 2854.468 kb on - strand, within MPMX26_02770 at 2854.468 kb on - strand, within MPMX26_02770 at 2854.468 kb on - strand, within MPMX26_02770 at 2854.510 kb on - strand, within MPMX26_02770 at 2854.510 kb on - strand, within MPMX26_02770 at 2854.533 kb on - strand, within MPMX26_02770 at 2854.538 kb on - strand, within MPMX26_02770 at 2854.547 kb on - strand, within MPMX26_02770 at 2854.578 kb on - strand, within MPMX26_02770 at 2854.586 kb on - strand, within MPMX26_02770 at 2854.603 kb on - strand, within MPMX26_02770 at 2854.633 kb on - strand at 2854.633 kb on - strand at 2854.690 kb on - strand at 2854.705 kb on - strand at 2854.705 kb on - strand at 2854.785 kb on - strand at 2854.805 kb on - strand at 2854.847 kb on - strand at 2854.847 kb on - strand at 2854.852 kb on + strand at 2854.860 kb on - strand at 2854.860 kb on - strand at 2854.860 kb on - strand at 2854.860 kb on - strand at 2854.860 kb on - strand at 2854.860 kb on - strand at 2854.860 kb on - strand at 2854.860 kb on - strand at 2854.860 kb on - strand at 2854.860 kb on - strand at 2854.860 kb on - strand at 2854.860 kb on - strand at 2854.860 kb on - strand at 2854.883 kb on - strand at 2854.930 kb on - strand at 2854.944 kb on - strand at 2854.944 kb on - strand at 2854.949 kb on - strand at 2854.949 kb on - strand at 2854.949 kb on - strand at 2854.949 kb on - strand at 2854.949 kb on - strand at 2854.949 kb on - strand at 2854.949 kb on - strand at 2854.949 kb on - strand at 2854.949 kb on - strand at 2854.954 kb on - strand at 2854.954 kb on - strand at 2854.959 kb on - strand at 2855.061 kb on - strand at 2855.061 kb on - strand at 2855.061 kb on - strand at 2855.149 kb on - strand at 2855.149 kb on - strand at 2855.149 kb on - strand at 2855.149 kb on - strand at 2855.149 kb on - strand at 2855.149 kb on - strand at 2855.210 kb on - strand, within MPMX26_02771 at 2855.210 kb on - strand, within MPMX26_02771 at 2855.210 kb on - strand, within MPMX26_02771 at 2855.262 kb on - strand, within MPMX26_02771 at 2855.303 kb on - strand, within MPMX26_02771 at 2855.303 kb on - strand, within MPMX26_02771 at 2855.318 kb on - strand, within MPMX26_02771 at 2855.318 kb on - strand, within MPMX26_02771 at 2855.353 kb on - strand, within MPMX26_02771 at 2855.365 kb on - strand, within MPMX26_02771 at 2855.372 kb on - strand, within MPMX26_02771 at 2855.372 kb on - strand, within MPMX26_02771 at 2855.372 kb on - strand, within MPMX26_02771 at 2855.372 kb on - strand, within MPMX26_02771 at 2855.372 kb on - strand, within MPMX26_02771 at 2855.374 kb on + strand, within MPMX26_02771 at 2855.377 kb on - strand, within MPMX26_02771 at 2855.382 kb on - strand, within MPMX26_02771 at 2855.382 kb on - strand, within MPMX26_02771 at 2855.382 kb on - strand, within MPMX26_02771 at 2855.382 kb on - strand, within MPMX26_02771 at 2855.395 kb on - strand, within MPMX26_02771 at 2855.407 kb on - strand, within MPMX26_02771 at 2855.409 kb on - strand, within MPMX26_02771 at 2855.568 kb on - strand at 2855.570 kb on - strand at 2855.577 kb on - strand at 2855.665 kb on - strand at 2855.665 kb on - strand at 2855.683 kb on - strand at 2855.690 kb on - strand at 2855.690 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB with Dermcidin 2 ug/mL remove 2,852,585 + MPMX26_02768 0.84 +0.7 2,852,585 + MPMX26_02768 0.84 -0.7 2,852,590 + MPMX26_02768 0.84 +0.1 2,852,591 + MPMX26_02768 0.84 +0.1 2,852,604 + MPMX26_02768 0.86 +0.4 2,852,618 + MPMX26_02768 0.87 -1.9 2,852,631 - MPMX26_02768 0.88 -0.1 2,852,692 + -1.6 2,852,714 + -0.3 2,852,786 + -0.4 2,852,814 + +0.3 2,852,819 + +0.0 2,852,819 + -1.3 2,852,835 + -0.4 2,852,835 + -0.4 2,852,835 + -0.4 2,852,840 + +0.1 2,852,933 + MPMX26_02769 0.11 -1.0 2,852,943 + MPMX26_02769 0.13 +1.2 2,852,943 + MPMX26_02769 0.13 -0.4 2,852,970 + MPMX26_02769 0.17 -0.6 2,852,970 + MPMX26_02769 0.17 +0.4 2,852,970 + MPMX26_02769 0.17 +0.5 2,852,970 + MPMX26_02769 0.17 -0.0 2,852,970 + MPMX26_02769 0.17 +0.7 2,852,972 + MPMX26_02769 0.18 +0.7 2,852,985 + MPMX26_02769 0.20 -0.3 2,852,999 + MPMX26_02769 0.23 -0.3 2,853,006 + MPMX26_02769 0.24 -0.0 2,853,006 + MPMX26_02769 0.24 -0.2 2,853,046 + MPMX26_02769 0.31 +0.4 2,853,046 + MPMX26_02769 0.31 -0.2 2,853,048 + MPMX26_02769 0.32 +0.3 2,853,048 + MPMX26_02769 0.32 +0.4 2,853,048 + MPMX26_02769 0.32 -1.7 2,853,048 + MPMX26_02769 0.32 +0.9 2,853,048 + MPMX26_02769 0.32 +0.8 2,853,055 + MPMX26_02769 0.33 -0.4 2,853,055 + MPMX26_02769 0.33 +0.9 2,853,055 + MPMX26_02769 0.33 -0.1 2,853,055 + MPMX26_02769 0.33 +0.4 2,853,077 + MPMX26_02769 0.37 -0.2 2,853,081 + MPMX26_02769 0.38 -1.2 2,853,086 + MPMX26_02769 0.39 -0.5 2,853,086 + MPMX26_02769 0.39 -0.5 2,853,086 + MPMX26_02769 0.39 +0.2 2,853,086 + MPMX26_02769 0.39 -0.1 2,853,086 + MPMX26_02769 0.39 +0.0 2,853,086 + MPMX26_02769 0.39 +0.1 2,853,086 + MPMX26_02769 0.39 -0.3 2,853,093 + MPMX26_02769 0.40 -0.3 2,853,103 + MPMX26_02769 0.42 +0.8 2,853,198 + MPMX26_02769 0.59 -0.2 2,853,198 + MPMX26_02769 0.59 -0.6 2,853,198 + MPMX26_02769 0.59 -0.1 2,853,198 + MPMX26_02769 0.59 -0.7 2,853,204 + MPMX26_02769 0.60 -0.9 2,853,204 + MPMX26_02769 0.60 +0.6 2,853,204 + MPMX26_02769 0.60 +2.9 2,853,204 + MPMX26_02769 0.60 -0.1 2,853,204 + MPMX26_02769 0.60 -0.5 2,853,204 + MPMX26_02769 0.60 -0.4 2,853,204 + MPMX26_02769 0.60 +0.0 2,853,204 + MPMX26_02769 0.60 +0.3 2,853,204 + MPMX26_02769 0.60 -0.3 2,853,204 + MPMX26_02769 0.60 -0.4 2,853,204 + MPMX26_02769 0.60 +0.2 2,853,204 + MPMX26_02769 0.60 -0.1 2,853,213 + MPMX26_02769 0.62 -0.1 2,853,213 + MPMX26_02769 0.62 -0.3 2,853,223 + MPMX26_02769 0.64 -0.2 2,853,276 + MPMX26_02769 0.73 +0.0 2,853,276 + MPMX26_02769 0.73 +0.2 2,853,276 + MPMX26_02769 0.73 +1.3 2,853,286 + MPMX26_02769 0.75 +3.9 2,853,297 + MPMX26_02769 0.77 -0.2 2,853,297 + MPMX26_02769 0.77 -0.6 2,853,314 + MPMX26_02769 0.80 -0.2 2,853,324 + MPMX26_02769 0.82 +0.2 2,853,335 + MPMX26_02769 0.84 -0.5 2,853,353 + MPMX26_02769 0.87 -0.3 2,853,358 + MPMX26_02769 0.88 +1.6 2,853,372 + -0.9 2,853,385 + +0.6 2,853,385 + +1.3 2,853,388 + +0.3 2,853,566 - -0.3 2,853,585 - -0.7 2,853,585 - -0.6 2,853,585 - +1.3 2,853,585 - +0.5 2,853,585 - -2.3 2,853,590 - +0.1 2,853,662 - MPMX26_02770 0.13 -1.4 2,853,679 - MPMX26_02770 0.15 -0.3 2,853,724 - MPMX26_02770 0.18 -0.2 2,853,724 - MPMX26_02770 0.18 +0.2 2,853,724 - MPMX26_02770 0.18 -0.3 2,853,724 - MPMX26_02770 0.18 -0.7 2,853,744 - MPMX26_02770 0.20 +1.9 2,853,744 - MPMX26_02770 0.20 +0.9 2,853,754 - MPMX26_02770 0.21 +0.4 2,853,765 - MPMX26_02770 0.22 +0.9 2,853,765 - MPMX26_02770 0.22 -2.0 2,853,765 - MPMX26_02770 0.22 +1.3 2,853,765 - MPMX26_02770 0.22 -0.1 2,853,775 - MPMX26_02770 0.23 -0.5 2,853,775 - MPMX26_02770 0.23 +0.3 2,853,775 - MPMX26_02770 0.23 -0.8 2,853,775 - MPMX26_02770 0.23 +0.9 2,853,818 - MPMX26_02770 0.26 +0.4 2,853,818 - MPMX26_02770 0.26 -0.3 2,853,818 - MPMX26_02770 0.26 +0.1 2,853,830 - MPMX26_02770 0.27 +0.4 2,853,865 - MPMX26_02770 0.30 -0.4 2,853,911 - MPMX26_02770 0.34 -0.1 2,853,911 - MPMX26_02770 0.34 -1.0 2,853,926 - MPMX26_02770 0.35 -1.2 2,853,926 - MPMX26_02770 0.35 -0.6 2,853,936 - MPMX26_02770 0.36 +1.9 2,854,013 - MPMX26_02770 0.42 -0.2 2,854,013 - MPMX26_02770 0.42 +1.1 2,854,013 - MPMX26_02770 0.42 -0.0 2,854,013 - MPMX26_02770 0.42 +0.4 2,854,013 - MPMX26_02770 0.42 +0.2 2,854,013 - MPMX26_02770 0.42 +0.1 2,854,013 - MPMX26_02770 0.42 -1.0 2,854,013 - MPMX26_02770 0.42 -0.9 2,854,013 - MPMX26_02770 0.42 -0.3 2,854,036 - MPMX26_02770 0.44 +1.0 2,854,129 - MPMX26_02770 0.51 -0.5 2,854,206 - MPMX26_02770 0.58 +0.3 2,854,223 - MPMX26_02770 0.59 +0.6 2,854,280 - MPMX26_02770 0.64 +0.9 2,854,300 - MPMX26_02770 0.65 +0.1 2,854,335 - MPMX26_02770 0.68 +0.3 2,854,335 - MPMX26_02770 0.68 +0.9 2,854,370 - MPMX26_02770 0.71 +0.1 2,854,386 - MPMX26_02770 0.72 +1.1 2,854,388 - MPMX26_02770 0.72 -0.3 2,854,468 - MPMX26_02770 0.79 +1.0 2,854,468 - MPMX26_02770 0.79 +0.3 2,854,468 - MPMX26_02770 0.79 -0.1 2,854,468 - MPMX26_02770 0.79 +0.6 2,854,468 - MPMX26_02770 0.79 -0.5 2,854,510 - MPMX26_02770 0.82 +0.5 2,854,510 - MPMX26_02770 0.82 +1.9 2,854,533 - MPMX26_02770 0.84 +0.3 2,854,538 - MPMX26_02770 0.85 +0.1 2,854,547 - MPMX26_02770 0.85 -1.0 2,854,578 - MPMX26_02770 0.88 +0.9 2,854,586 - MPMX26_02770 0.89 -0.5 2,854,603 - MPMX26_02770 0.90 -1.0 2,854,633 - -0.4 2,854,633 - -1.5 2,854,690 - -0.8 2,854,705 - -0.4 2,854,705 - -0.5 2,854,785 - +1.5 2,854,805 - -0.1 2,854,847 - +0.0 2,854,847 - -0.0 2,854,852 + -0.3 2,854,860 - -0.1 2,854,860 - +0.4 2,854,860 - +0.9 2,854,860 - +0.0 2,854,860 - -0.5 2,854,860 - -0.9 2,854,860 - -0.2 2,854,860 - -0.3 2,854,860 - +0.1 2,854,860 - -0.2 2,854,860 - -0.9 2,854,860 - -0.3 2,854,860 - -0.8 2,854,883 - -0.9 2,854,930 - +0.5 2,854,944 - -0.7 2,854,944 - +0.6 2,854,949 - -0.2 2,854,949 - -0.2 2,854,949 - -0.4 2,854,949 - -0.2 2,854,949 - +0.3 2,854,949 - +0.1 2,854,949 - -0.5 2,854,949 - +0.3 2,854,949 - +0.8 2,854,954 - -0.2 2,854,954 - -1.1 2,854,959 - -1.6 2,855,061 - +0.2 2,855,061 - +1.9 2,855,061 - +0.3 2,855,149 - -0.0 2,855,149 - +0.5 2,855,149 - -0.4 2,855,149 - +0.9 2,855,149 - -1.3 2,855,149 - -0.8 2,855,210 - MPMX26_02771 0.20 -0.7 2,855,210 - MPMX26_02771 0.20 -0.7 2,855,210 - MPMX26_02771 0.20 -0.5 2,855,262 - MPMX26_02771 0.35 -0.1 2,855,303 - MPMX26_02771 0.46 +0.4 2,855,303 - MPMX26_02771 0.46 +0.0 2,855,318 - MPMX26_02771 0.50 +0.3 2,855,318 - MPMX26_02771 0.50 -2.3 2,855,353 - MPMX26_02771 0.60 -0.1 2,855,365 - MPMX26_02771 0.63 -0.7 2,855,372 - MPMX26_02771 0.65 -0.2 2,855,372 - MPMX26_02771 0.65 -0.3 2,855,372 - MPMX26_02771 0.65 +0.6 2,855,372 - MPMX26_02771 0.65 -0.1 2,855,372 - MPMX26_02771 0.65 +0.3 2,855,374 + MPMX26_02771 0.65 +1.6 2,855,377 - MPMX26_02771 0.66 +0.1 2,855,382 - MPMX26_02771 0.67 +0.9 2,855,382 - MPMX26_02771 0.67 +1.3 2,855,382 - MPMX26_02771 0.67 -1.3 2,855,382 - MPMX26_02771 0.67 +0.3 2,855,395 - MPMX26_02771 0.71 -0.4 2,855,407 - MPMX26_02771 0.74 -0.4 2,855,409 - MPMX26_02771 0.75 -0.6 2,855,568 - -0.7 2,855,570 - -0.3 2,855,577 - -0.1 2,855,665 - +0.6 2,855,665 - +0.9 2,855,683 - +0.1 2,855,690 - -2.3 2,855,690 - -0.8
Or see this region's nucleotide sequence