Experiment: Control_ECRC98_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisIE and hisF overlap by 7 nucleotides hisF and hisA overlap by 19 nucleotides hisA and hisH overlap by 1 nucleotides hisH and hisB overlap by 1 nucleotides
JDDGAC_28595: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 5,489,946 to 5,490,557
hisIE
JDDGAC_28600: hisF - Imidazole glycerol phosphate synthase subunit HisF, at 5,490,551 to 5,491,327
hisF
JDDGAC_28605: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 5,491,309 to 5,492,046
hisA
JDDGAC_28610: hisH - imidazole glycerol phosphate synthase subunit HisH, at 5,492,046 to 5,492,636
hisH
JDDGAC_28615: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 5,492,636 to 5,493,703
hisB
Position (kb) 5491
5492
5493 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 5490.337 kb on - strand, within hisIE at 5490.387 kb on - strand, within hisIE at 5490.451 kb on + strand, within hisIE at 5490.608 kb on - strand at 5490.938 kb on - strand, within hisF at 5491.146 kb on - strand, within hisF at 5491.311 kb on - strand at 5491.364 kb on - strand at 5491.490 kb on - strand, within hisA at 5491.490 kb on - strand, within hisA at 5491.851 kb on + strand, within hisA at 5492.024 kb on - strand at 5492.127 kb on - strand, within hisH at 5492.161 kb on + strand, within hisH at 5492.165 kb on + strand, within hisH at 5492.166 kb on - strand, within hisH at 5492.564 kb on - strand, within hisH at 5492.616 kb on - strand at 5492.637 kb on + strand at 5492.637 kb on + strand at 5492.746 kb on + strand, within hisB at 5492.784 kb on + strand, within hisB at 5492.844 kb on + strand, within hisB at 5492.860 kb on + strand, within hisB at 5493.031 kb on - strand, within hisB at 5493.031 kb on - strand, within hisB
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC98_WM remove 5,490,337 - hisIE JDDGAC_28595 0.64 -2.4 5,490,387 - hisIE JDDGAC_28595 0.72 -0.2 5,490,451 + hisIE JDDGAC_28595 0.83 -1.8 5,490,608 - -3.6 5,490,938 - hisF JDDGAC_28600 0.50 -2.2 5,491,146 - hisF JDDGAC_28600 0.77 -1.5 5,491,311 - -3.6 5,491,364 - -2.8 5,491,490 - hisA JDDGAC_28605 0.25 -3.1 5,491,490 - hisA JDDGAC_28605 0.25 -4.8 5,491,851 + hisA JDDGAC_28605 0.73 -2.7 5,492,024 - -2.0 5,492,127 - hisH JDDGAC_28610 0.14 -4.4 5,492,161 + hisH JDDGAC_28610 0.19 -2.7 5,492,165 + hisH JDDGAC_28610 0.20 -2.7 5,492,166 - hisH JDDGAC_28610 0.20 -3.1 5,492,564 - hisH JDDGAC_28610 0.88 -3.0 5,492,616 - -3.5 5,492,637 + -3.6 5,492,637 + -3.4 5,492,746 + hisB JDDGAC_28615 0.10 -4.0 5,492,784 + hisB JDDGAC_28615 0.14 +0.2 5,492,844 + hisB JDDGAC_28615 0.19 -2.9 5,492,860 + hisB JDDGAC_28615 0.21 -3.0 5,493,031 - hisB JDDGAC_28615 0.37 -4.6 5,493,031 - hisB JDDGAC_28615 0.37 -1.7
Or see this region's nucleotide sequence