Experiment: Control_ECRC98_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpiA and serA are separated by 254 nucleotides serA and JDDGAC_23110 are separated by 228 nucleotides JDDGAC_23110 and JDDGAC_23120 are separated by 333 nucleotides JDDGAC_23120 and ygfA are separated by 100 nucleotides
JDDGAC_23095: rpiA - ribose-5-phosphate isomerase RpiA, at 4,421,145 to 4,421,804
rpiA
JDDGAC_23100: serA - phosphoglycerate dehydrogenase, at 4,422,059 to 4,423,291
serA
JDDGAC_23110: JDDGAC_23110 - Type I toxin-antitoxin system Ibs family toxin, at 4,423,520 to 4,423,579
_23110
JDDGAC_23120: JDDGAC_23120 - Type I toxin-antitoxin system Ibs family toxin, at 4,423,913 to 4,423,972
_23120
JDDGAC_23125: ygfA - 5-formyltetrahydrofolate cyclo-ligase, at 4,424,073 to 4,424,675
ygfA
Position (kb)
4422
4423
4424 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 4421.106 kb on - strand at 4421.107 kb on + strand at 4421.107 kb on + strand at 4421.107 kb on + strand at 4421.107 kb on + strand at 4421.107 kb on + strand at 4421.107 kb on + strand at 4421.107 kb on + strand at 4421.901 kb on - strand at 4421.919 kb on + strand at 4421.920 kb on - strand at 4421.992 kb on + strand at 4422.003 kb on - strand at 4422.070 kb on - strand at 4422.091 kb on + strand at 4422.092 kb on - strand at 4422.092 kb on - strand at 4422.105 kb on + strand at 4422.152 kb on + strand at 4422.153 kb on - strand at 4422.307 kb on + strand, within serA at 4422.309 kb on + strand, within serA at 4422.426 kb on + strand, within serA at 4422.542 kb on - strand, within serA at 4422.584 kb on + strand, within serA at 4422.584 kb on + strand, within serA at 4422.584 kb on + strand, within serA at 4422.585 kb on - strand, within serA at 4422.585 kb on - strand, within serA at 4422.585 kb on - strand, within serA at 4422.591 kb on - strand, within serA at 4422.591 kb on - strand, within serA at 4422.597 kb on - strand, within serA at 4422.597 kb on - strand, within serA at 4422.664 kb on + strand, within serA at 4422.691 kb on - strand, within serA at 4422.715 kb on + strand, within serA at 4422.766 kb on + strand, within serA at 4422.878 kb on - strand, within serA at 4422.890 kb on - strand, within serA at 4423.001 kb on + strand, within serA at 4423.110 kb on + strand, within serA at 4423.163 kb on + strand, within serA at 4423.163 kb on + strand, within serA at 4423.168 kb on - strand at 4423.194 kb on + strand at 4423.194 kb on + strand at 4423.194 kb on + strand at 4423.194 kb on + strand at 4423.194 kb on + strand at 4423.194 kb on + strand at 4423.194 kb on + strand at 4423.195 kb on - strand at 4423.195 kb on - strand at 4423.195 kb on - strand at 4423.195 kb on - strand at 4423.199 kb on - strand at 4423.290 kb on - strand at 4423.290 kb on - strand at 4423.290 kb on - strand at 4423.378 kb on + strand at 4423.539 kb on + strand, within JDDGAC_23110 at 4423.539 kb on + strand, within JDDGAC_23110 at 4423.575 kb on - strand at 4423.575 kb on - strand at 4423.575 kb on - strand at 4423.575 kb on - strand at 4423.578 kb on - strand at 4423.753 kb on + strand at 4423.753 kb on + strand at 4423.753 kb on + strand at 4423.753 kb on + strand at 4423.753 kb on + strand at 4423.754 kb on - strand at 4423.754 kb on - strand at 4423.754 kb on - strand at 4423.765 kb on - strand at 4423.807 kb on + strand at 4423.830 kb on + strand at 4423.842 kb on - strand at 4423.932 kb on + strand, within JDDGAC_23120 at 4423.941 kb on + strand, within JDDGAC_23120 at 4423.941 kb on + strand, within JDDGAC_23120 at 4423.947 kb on + strand, within JDDGAC_23120 at 4423.947 kb on + strand, within JDDGAC_23120 at 4423.968 kb on - strand at 4423.968 kb on - strand at 4424.066 kb on - strand at 4424.190 kb on - strand, within ygfA at 4424.190 kb on - strand, within ygfA at 4424.190 kb on - strand, within ygfA at 4424.192 kb on - strand, within ygfA at 4424.208 kb on + strand, within ygfA
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC98_WM remove 4,421,106 - +0.7 4,421,107 + -0.1 4,421,107 + -2.4 4,421,107 + -1.2 4,421,107 + +0.2 4,421,107 + -1.4 4,421,107 + +0.6 4,421,107 + +0.1 4,421,901 - -0.1 4,421,919 + -0.5 4,421,920 - -0.6 4,421,992 + -4.2 4,422,003 - -4.1 4,422,070 - -3.5 4,422,091 + -2.7 4,422,092 - -5.3 4,422,092 - -2.1 4,422,105 + -4.7 4,422,152 + -0.7 4,422,153 - -4.7 4,422,307 + serA JDDGAC_23100 0.20 -3.5 4,422,309 + serA JDDGAC_23100 0.20 -3.9 4,422,426 + serA JDDGAC_23100 0.30 -1.2 4,422,542 - serA JDDGAC_23100 0.39 -4.3 4,422,584 + serA JDDGAC_23100 0.43 -3.0 4,422,584 + serA JDDGAC_23100 0.43 -2.9 4,422,584 + serA JDDGAC_23100 0.43 -2.9 4,422,585 - serA JDDGAC_23100 0.43 -3.2 4,422,585 - serA JDDGAC_23100 0.43 -2.4 4,422,585 - serA JDDGAC_23100 0.43 -3.7 4,422,591 - serA JDDGAC_23100 0.43 -5.3 4,422,591 - serA JDDGAC_23100 0.43 -3.9 4,422,597 - serA JDDGAC_23100 0.44 -2.2 4,422,597 - serA JDDGAC_23100 0.44 -6.1 4,422,664 + serA JDDGAC_23100 0.49 -5.0 4,422,691 - serA JDDGAC_23100 0.51 -3.7 4,422,715 + serA JDDGAC_23100 0.53 -5.3 4,422,766 + serA JDDGAC_23100 0.57 -4.4 4,422,878 - serA JDDGAC_23100 0.66 -2.0 4,422,890 - serA JDDGAC_23100 0.67 -4.3 4,423,001 + serA JDDGAC_23100 0.76 -2.7 4,423,110 + serA JDDGAC_23100 0.85 -3.7 4,423,163 + serA JDDGAC_23100 0.90 -3.7 4,423,163 + serA JDDGAC_23100 0.90 -1.0 4,423,168 - -3.2 4,423,194 + -5.4 4,423,194 + -3.1 4,423,194 + -4.6 4,423,194 + -2.3 4,423,194 + -3.0 4,423,194 + -2.3 4,423,194 + -5.0 4,423,195 - -1.2 4,423,195 - -3.4 4,423,195 - -2.8 4,423,195 - -4.4 4,423,199 - -2.5 4,423,290 - +0.0 4,423,290 - +0.3 4,423,290 - -0.6 4,423,378 + -1.1 4,423,539 + JDDGAC_23110 0.32 -1.3 4,423,539 + JDDGAC_23110 0.32 +0.9 4,423,575 - -1.3 4,423,575 - +1.6 4,423,575 - -0.4 4,423,575 - -0.2 4,423,578 - -1.0 4,423,753 + -0.3 4,423,753 + +0.8 4,423,753 + -0.0 4,423,753 + +0.4 4,423,753 + +0.5 4,423,754 - +1.0 4,423,754 - -2.7 4,423,754 - -0.3 4,423,765 - +1.2 4,423,807 + +0.5 4,423,830 + +0.2 4,423,842 - -0.0 4,423,932 + JDDGAC_23120 0.32 +1.0 4,423,941 + JDDGAC_23120 0.47 +0.1 4,423,941 + JDDGAC_23120 0.47 -1.2 4,423,947 + JDDGAC_23120 0.57 +0.7 4,423,947 + JDDGAC_23120 0.57 +0.0 4,423,968 - +0.2 4,423,968 - -2.1 4,424,066 - +0.1 4,424,190 - ygfA JDDGAC_23125 0.19 -0.4 4,424,190 - ygfA JDDGAC_23125 0.19 +1.0 4,424,190 - ygfA JDDGAC_23125 0.19 -0.2 4,424,192 - ygfA JDDGAC_23125 0.20 -3.2 4,424,208 + ygfA JDDGAC_23125 0.22 -1.0
Or see this region's nucleotide sequence