Experiment: Control_ECRC98_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysG and nirC are separated by 18 nucleotides nirC and nirD are separated by 125 nucleotides nirD and nirB overlap by 4 nucleotides
JDDGAC_20810: cysG - siroheme synthase CysG, at 3,998,897 to 4,000,270
cysG
JDDGAC_20815: nirC - nitrite transporter NirC, at 4,000,289 to 4,001,095
nirC
JDDGAC_20820: nirD - nitrite reductase small subunit NirD, at 4,001,221 to 4,001,547
nirD
JDDGAC_20825: nirB - NADPH-nitrite reductase large subunit, at 4,001,544 to 4,004,087
nirB
Position (kb) 4001
4002 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4000.253 kb on + strand at 4000.254 kb on - strand at 4000.254 kb on - strand at 4000.287 kb on - strand at 4000.287 kb on - strand at 4000.290 kb on + strand at 4000.351 kb on + strand at 4000.351 kb on + strand at 4000.356 kb on + strand at 4000.356 kb on + strand at 4000.356 kb on + strand at 4000.357 kb on - strand at 4000.379 kb on + strand, within nirC at 4000.379 kb on + strand, within nirC at 4000.380 kb on - strand, within nirC at 4000.396 kb on + strand, within nirC at 4000.396 kb on + strand, within nirC at 4000.397 kb on - strand, within nirC at 4000.397 kb on - strand, within nirC at 4000.397 kb on - strand, within nirC at 4000.397 kb on - strand, within nirC at 4000.640 kb on + strand, within nirC at 4000.641 kb on - strand, within nirC at 4000.694 kb on - strand, within nirC at 4000.694 kb on - strand, within nirC at 4000.726 kb on + strand, within nirC at 4000.726 kb on + strand, within nirC at 4000.726 kb on + strand, within nirC at 4000.727 kb on - strand, within nirC at 4000.728 kb on + strand, within nirC at 4000.728 kb on + strand, within nirC at 4000.728 kb on + strand, within nirC at 4000.890 kb on - strand, within nirC at 4000.987 kb on - strand, within nirC at 4000.987 kb on - strand, within nirC at 4001.074 kb on + strand at 4001.080 kb on + strand at 4001.162 kb on - strand at 4001.174 kb on + strand at 4001.264 kb on + strand, within nirD at 4001.265 kb on - strand, within nirD at 4001.312 kb on - strand, within nirD at 4001.480 kb on - strand, within nirD at 4001.568 kb on + strand at 4001.576 kb on + strand at 4001.577 kb on - strand at 4001.718 kb on + strand at 4001.827 kb on - strand, within nirB at 4001.862 kb on - strand, within nirB at 4001.869 kb on + strand, within nirB at 4001.870 kb on - strand, within nirB at 4001.870 kb on - strand, within nirB at 4001.870 kb on - strand, within nirB at 4001.894 kb on - strand, within nirB at 4001.974 kb on + strand, within nirB at 4001.974 kb on + strand, within nirB at 4001.974 kb on + strand, within nirB at 4001.974 kb on + strand, within nirB at 4002.080 kb on + strand, within nirB at 4002.081 kb on - strand, within nirB at 4002.307 kb on + strand, within nirB at 4002.308 kb on - strand, within nirB at 4002.308 kb on - strand, within nirB at 4002.395 kb on - strand, within nirB at 4002.472 kb on + strand, within nirB at 4002.515 kb on + strand, within nirB
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC98_WM remove 4,000,253 + -3.5 4,000,254 - -0.7 4,000,254 - -2.1 4,000,287 - -0.6 4,000,287 - +1.4 4,000,290 + -0.6 4,000,351 + -0.5 4,000,351 + -0.2 4,000,356 + -1.2 4,000,356 + -0.6 4,000,356 + -0.0 4,000,357 - +0.7 4,000,379 + nirC JDDGAC_20815 0.11 +0.4 4,000,379 + nirC JDDGAC_20815 0.11 -0.1 4,000,380 - nirC JDDGAC_20815 0.11 +0.2 4,000,396 + nirC JDDGAC_20815 0.13 -0.3 4,000,396 + nirC JDDGAC_20815 0.13 +0.7 4,000,397 - nirC JDDGAC_20815 0.13 -0.1 4,000,397 - nirC JDDGAC_20815 0.13 +0.7 4,000,397 - nirC JDDGAC_20815 0.13 +0.2 4,000,397 - nirC JDDGAC_20815 0.13 +0.5 4,000,640 + nirC JDDGAC_20815 0.43 +0.1 4,000,641 - nirC JDDGAC_20815 0.44 +0.1 4,000,694 - nirC JDDGAC_20815 0.50 +0.1 4,000,694 - nirC JDDGAC_20815 0.50 +0.7 4,000,726 + nirC JDDGAC_20815 0.54 -0.5 4,000,726 + nirC JDDGAC_20815 0.54 +0.3 4,000,726 + nirC JDDGAC_20815 0.54 -3.3 4,000,727 - nirC JDDGAC_20815 0.54 -0.1 4,000,728 + nirC JDDGAC_20815 0.54 +0.7 4,000,728 + nirC JDDGAC_20815 0.54 +0.0 4,000,728 + nirC JDDGAC_20815 0.54 -0.1 4,000,890 - nirC JDDGAC_20815 0.74 +0.8 4,000,987 - nirC JDDGAC_20815 0.86 +0.3 4,000,987 - nirC JDDGAC_20815 0.86 +0.2 4,001,074 + +0.1 4,001,080 + +0.5 4,001,162 - -0.5 4,001,174 + +1.4 4,001,264 + nirD JDDGAC_20820 0.13 +0.7 4,001,265 - nirD JDDGAC_20820 0.13 -1.0 4,001,312 - nirD JDDGAC_20820 0.28 -1.0 4,001,480 - nirD JDDGAC_20820 0.79 -0.3 4,001,568 + +0.5 4,001,576 + +0.8 4,001,577 - +0.7 4,001,718 + -0.2 4,001,827 - nirB JDDGAC_20825 0.11 -4.5 4,001,862 - nirB JDDGAC_20825 0.12 -1.2 4,001,869 + nirB JDDGAC_20825 0.13 -0.3 4,001,870 - nirB JDDGAC_20825 0.13 +0.1 4,001,870 - nirB JDDGAC_20825 0.13 +0.6 4,001,870 - nirB JDDGAC_20825 0.13 +0.0 4,001,894 - nirB JDDGAC_20825 0.14 -1.4 4,001,974 + nirB JDDGAC_20825 0.17 +0.4 4,001,974 + nirB JDDGAC_20825 0.17 -1.3 4,001,974 + nirB JDDGAC_20825 0.17 -1.3 4,001,974 + nirB JDDGAC_20825 0.17 -0.3 4,002,080 + nirB JDDGAC_20825 0.21 +0.1 4,002,081 - nirB JDDGAC_20825 0.21 +0.7 4,002,307 + nirB JDDGAC_20825 0.30 -0.1 4,002,308 - nirB JDDGAC_20825 0.30 +0.1 4,002,308 - nirB JDDGAC_20825 0.30 +0.4 4,002,395 - nirB JDDGAC_20825 0.33 -0.2 4,002,472 + nirB JDDGAC_20825 0.36 -0.6 4,002,515 + nirB JDDGAC_20825 0.38 -2.9
Or see this region's nucleotide sequence