Strain Fitness in Escherichia coli ECRC98 around JDDGAC_19305

Experiment: Control_ECRC98_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfaJ and rfaB are separated by 8 nucleotidesrfaB and rfaL are separated by 35 nucleotides JDDGAC_19300: rfaJ - glucosyltransferase, at 3,670,800 to 3,671,813 rfaJ JDDGAC_19305: rfaB - UDP-glucose--(glucosyl)LPS alpha-1,2-glucosyltransferase, at 3,671,822 to 3,672,964 rfaB JDDGAC_19310: rfaL - O-antigen ligase RfaL, at 3,673,000 to 3,674,208 rfaL Position (kb) 3671 3672 3673Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3670.984 kb on + strand, within rfaJat 3671.010 kb on + strand, within rfaJat 3671.179 kb on + strand, within rfaJat 3671.310 kb on - strand, within rfaJat 3671.513 kb on + strand, within rfaJat 3671.593 kb on + strand, within rfaJat 3671.598 kb on + strand, within rfaJat 3671.637 kb on + strand, within rfaJat 3671.904 kb on - strandat 3672.072 kb on - strand, within rfaBat 3672.198 kb on + strand, within rfaBat 3672.386 kb on - strand, within rfaBat 3672.601 kb on + strand, within rfaBat 3672.601 kb on + strand, within rfaBat 3672.601 kb on + strand, within rfaBat 3672.602 kb on - strand, within rfaBat 3672.607 kb on - strand, within rfaBat 3672.712 kb on + strand, within rfaBat 3672.779 kb on - strand, within rfaBat 3672.806 kb on - strand, within rfaBat 3672.905 kb on + strandat 3672.962 kb on + strandat 3673.189 kb on + strand, within rfaLat 3673.216 kb on + strand, within rfaLat 3673.261 kb on + strand, within rfaLat 3673.272 kb on + strand, within rfaLat 3673.305 kb on + strand, within rfaLat 3673.323 kb on - strand, within rfaLat 3673.437 kb on - strand, within rfaL

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC98_WM
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3,670,984 + rfaJ JDDGAC_19300 0.18 -0.2
3,671,010 + rfaJ JDDGAC_19300 0.21 -2.8
3,671,179 + rfaJ JDDGAC_19300 0.37 +0.8
3,671,310 - rfaJ JDDGAC_19300 0.50 -1.3
3,671,513 + rfaJ JDDGAC_19300 0.70 +0.1
3,671,593 + rfaJ JDDGAC_19300 0.78 -1.6
3,671,598 + rfaJ JDDGAC_19300 0.79 -3.8
3,671,637 + rfaJ JDDGAC_19300 0.83 -2.9
3,671,904 - -1.6
3,672,072 - rfaB JDDGAC_19305 0.22 -1.3
3,672,198 + rfaB JDDGAC_19305 0.33 -3.8
3,672,386 - rfaB JDDGAC_19305 0.49 -3.0
3,672,601 + rfaB JDDGAC_19305 0.68 -3.1
3,672,601 + rfaB JDDGAC_19305 0.68 -2.6
3,672,601 + rfaB JDDGAC_19305 0.68 -2.6
3,672,602 - rfaB JDDGAC_19305 0.68 -3.4
3,672,607 - rfaB JDDGAC_19305 0.69 -1.7
3,672,712 + rfaB JDDGAC_19305 0.78 -2.3
3,672,779 - rfaB JDDGAC_19305 0.84 +0.6
3,672,806 - rfaB JDDGAC_19305 0.86 -2.2
3,672,905 + -4.1
3,672,962 + +0.5
3,673,189 + rfaL JDDGAC_19310 0.16 -1.4
3,673,216 + rfaL JDDGAC_19310 0.18 -2.3
3,673,261 + rfaL JDDGAC_19310 0.22 -3.5
3,673,272 + rfaL JDDGAC_19310 0.22 -3.0
3,673,305 + rfaL JDDGAC_19310 0.25 -1.2
3,673,323 - rfaL JDDGAC_19310 0.27 -1.3
3,673,437 - rfaL JDDGAC_19310 0.36 -2.4

Or see this region's nucleotide sequence