Strain Fitness in Escherichia coli ECRC98 around JDDGAC_05220

Experiment: Control_ECRC98_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrpB and trpA overlap by 1 nucleotidestrpA and yciG are separated by 380 nucleotidesyciG and yciF are separated by 85 nucleotides JDDGAC_05215: trpB - tryptophan synthase subunit beta, at 918,474 to 919,667 trpB JDDGAC_05220: trpA - tryptophan synthase subunit alpha, at 919,667 to 920,473 trpA JDDGAC_05225: yciG - Uncharacterized protein YciG, at 920,854 to 921,033 yciG JDDGAC_05230: yciF - Protein YciF, at 921,119 to 921,619 yciF Position (kb) 919 920 921Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 918.731 kb on + strand, within trpBat 918.871 kb on - strand, within trpBat 919.015 kb on - strand, within trpBat 919.029 kb on + strand, within trpBat 919.030 kb on - strand, within trpBat 919.030 kb on - strand, within trpBat 919.031 kb on + strand, within trpBat 919.286 kb on - strand, within trpBat 919.309 kb on - strand, within trpBat 919.443 kb on + strand, within trpBat 919.585 kb on + strandat 919.585 kb on + strandat 919.641 kb on - strandat 919.971 kb on - strand, within trpAat 920.164 kb on - strand, within trpAat 920.184 kb on - strand, within trpAat 920.306 kb on - strand, within trpAat 920.326 kb on - strand, within trpAat 920.550 kb on - strandat 920.595 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC98_WM
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918,731 + trpB JDDGAC_05215 0.22 -4.0
918,871 - trpB JDDGAC_05215 0.33 -3.0
919,015 - trpB JDDGAC_05215 0.45 -3.2
919,029 + trpB JDDGAC_05215 0.46 -4.2
919,030 - trpB JDDGAC_05215 0.47 -1.2
919,030 - trpB JDDGAC_05215 0.47 -3.3
919,031 + trpB JDDGAC_05215 0.47 -2.1
919,286 - trpB JDDGAC_05215 0.68 -3.6
919,309 - trpB JDDGAC_05215 0.70 -1.9
919,443 + trpB JDDGAC_05215 0.81 -3.6
919,585 + -3.9
919,585 + -1.3
919,641 - -4.8
919,971 - trpA JDDGAC_05220 0.38 -3.2
920,164 - trpA JDDGAC_05220 0.62 -1.0
920,184 - trpA JDDGAC_05220 0.64 -2.5
920,306 - trpA JDDGAC_05220 0.79 -3.7
920,326 - trpA JDDGAC_05220 0.82 -3.1
920,550 - -0.1
920,595 - -0.5

Or see this region's nucleotide sequence