Strain Fitness in Escherichia coli ECRC98 around JDDGAC_03230

Experiment: Control_ECRC98_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntJDDGAC_03210 and JDDGAC_03215 are separated by 50 nucleotidesJDDGAC_03215 and JDDGAC_03220 are separated by 33 nucleotidesJDDGAC_03220 and JDDGAC_03225 overlap by 8 nucleotidesJDDGAC_03225 and JDDGAC_03230 overlap by 4 nucleotidesJDDGAC_03230 and JDDGAC_03235 overlap by 23 nucleotidesJDDGAC_03235 and JDDGAC_03240 are separated by 48 nucleotidesJDDGAC_03240 and JDDGAC_03245 are separated by 200 nucleotides JDDGAC_03205: JDDGAC_03205 - Ead/Ea22-like family protein, at 542,886 to 542,999 _03205 JDDGAC_03210: JDDGAC_03210 - hypothetical protein, at 542,977 to 543,333 _03210 JDDGAC_03215: JDDGAC_03215 - hypothetical protein, at 543,384 to 543,596 _03215 JDDGAC_03220: JDDGAC_03220 - hypothetical protein, at 543,630 to 543,812 _03220 JDDGAC_03225: JDDGAC_03225 - hypothetical protein, at 543,805 to 543,981 _03225 JDDGAC_03230: JDDGAC_03230 - Ead/Ea22-like protein, at 543,978 to 544,697 _03230 JDDGAC_03235: JDDGAC_03235 - Ead/Ea22-like protein, at 544,675 to 545,031 _03235 JDDGAC_03240: JDDGAC_03240 - hypothetical protein, at 545,080 to 545,292 _03240 JDDGAC_03245: JDDGAC_03245 - DUF551 domain-containing protein, at 545,493 to 546,206 _03245 Position (kb) 543 544 545Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 543.224 kb on + strand, within JDDGAC_03210at 544.052 kb on + strand, within JDDGAC_03230at 544.052 kb on + strand, within JDDGAC_03230at 544.052 kb on + strand, within JDDGAC_03230at 544.194 kb on - strand, within JDDGAC_03230at 544.258 kb on - strand, within JDDGAC_03230at 545.480 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC98_WM
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543,224 + JDDGAC_03210 0.69 -0.2
544,052 + JDDGAC_03230 0.10 -0.5
544,052 + JDDGAC_03230 0.10 +1.4
544,052 + JDDGAC_03230 0.10 -2.3
544,194 - JDDGAC_03230 0.30 -2.0
544,258 - JDDGAC_03230 0.39 -4.4
545,480 + -0.5

Or see this region's nucleotide sequence