Experiment: TSB with Dermcidin 1 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX26_01462 and MPMX26_01463 overlap by 4 nucleotides MPMX26_01463 and MPMX26_01464 are separated by 12 nucleotides MPMX26_01464 and MPMX26_01465 are separated by 186 nucleotides MPMX26_01465 and MPMX26_01466 are separated by 81 nucleotides
MPMX26_01462: MPMX26_01462 - hypothetical protein, at 1,513,112 to 1,514,233
_01462
MPMX26_01463: MPMX26_01463 - hypothetical protein, at 1,514,230 to 1,514,928
_01463
MPMX26_01464: MPMX26_01464 - putative oxidoreductase, at 1,514,941 to 1,515,684
_01464
MPMX26_01465: MPMX26_01465 - hypothetical protein, at 1,515,871 to 1,516,461
_01465
MPMX26_01466: MPMX26_01466 - Bifunctional protein PutA, at 1,516,543 to 1,520,295
_01466
Position (kb)
1514
1515
1516 Strain fitness (log2 ratio)
-1
0
1
2 at 1514.943 kb on + strand at 1515.092 kb on + strand, within MPMX26_01464 at 1515.210 kb on + strand, within MPMX26_01464 at 1515.294 kb on + strand, within MPMX26_01464 at 1515.294 kb on + strand, within MPMX26_01464 at 1515.387 kb on + strand, within MPMX26_01464 at 1515.451 kb on + strand, within MPMX26_01464 at 1516.541 kb on + strand at 1516.676 kb on - strand at 1516.676 kb on - strand at 1516.684 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB with Dermcidin 1 ug/mL remove 1,514,943 + +0.2 1,515,092 + MPMX26_01464 0.20 +0.7 1,515,210 + MPMX26_01464 0.36 +0.4 1,515,294 + MPMX26_01464 0.47 +1.0 1,515,294 + MPMX26_01464 0.47 +2.6 1,515,387 + MPMX26_01464 0.60 +1.4 1,515,451 + MPMX26_01464 0.69 +2.6 1,516,541 + +0.4 1,516,676 - -0.7 1,516,676 - +0.0 1,516,684 - -0.9
Or see this region's nucleotide sequence