Strain Fitness in Escherichia coli ECRC98 around JDDGAC_28430

Experiment: WM_JK32

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwzc and wcaA are separated by 92 nucleotideswcaA and wcaB are separated by 2 nucleotideswcaB and wcaC overlap by 4 nucleotides JDDGAC_28420: wzc - tyrosine-protein kinase Wzc, at 5,448,209 to 5,450,371 wzc JDDGAC_28425: wcaA - colanic acid biosynthesis glycosyltransferase WcaA, at 5,450,464 to 5,451,303 wcaA JDDGAC_28430: wcaB - colanic acid biosynthesis acetyltransferase WcaB, at 5,451,306 to 5,451,794 wcaB JDDGAC_28435: wcaC - colanic acid biosynthesis glycosyltransferase WcaC, at 5,451,791 to 5,453,008 wcaC Position (kb) 5451 5452Strain fitness (log2 ratio) -2 -1 0 1at 5450.331 kb on - strandat 5450.339 kb on + strandat 5450.340 kb on - strandat 5450.352 kb on - strandat 5450.353 kb on + strandat 5450.457 kb on - strandat 5450.464 kb on - strandat 5450.557 kb on + strand, within wcaAat 5450.649 kb on + strand, within wcaAat 5450.649 kb on + strand, within wcaAat 5450.649 kb on + strand, within wcaAat 5450.653 kb on + strand, within wcaAat 5450.654 kb on - strand, within wcaAat 5450.695 kb on - strand, within wcaAat 5450.725 kb on + strand, within wcaAat 5450.726 kb on - strand, within wcaAat 5450.834 kb on - strand, within wcaAat 5450.873 kb on + strand, within wcaAat 5450.873 kb on + strand, within wcaAat 5450.874 kb on - strand, within wcaAat 5450.926 kb on - strand, within wcaAat 5450.926 kb on - strand, within wcaAat 5451.007 kb on + strand, within wcaAat 5451.133 kb on + strand, within wcaAat 5451.133 kb on + strand, within wcaAat 5451.134 kb on - strand, within wcaAat 5451.306 kb on - strandat 5451.516 kb on - strand, within wcaBat 5451.548 kb on + strand, within wcaBat 5451.549 kb on - strand, within wcaBat 5451.808 kb on + strandat 5451.964 kb on + strand, within wcaCat 5451.991 kb on + strand, within wcaCat 5452.045 kb on - strand, within wcaCat 5452.047 kb on - strand, within wcaCat 5452.114 kb on - strand, within wcaCat 5452.279 kb on + strand, within wcaCat 5452.279 kb on + strand, within wcaCat 5452.279 kb on + strand, within wcaCat 5452.280 kb on - strand, within wcaCat 5452.280 kb on - strand, within wcaCat 5452.283 kb on + strand, within wcaCat 5452.438 kb on + strand, within wcaCat 5452.444 kb on + strand, within wcaCat 5452.562 kb on - strand, within wcaCat 5452.616 kb on - strand, within wcaCat 5452.616 kb on - strand, within wcaCat 5452.616 kb on - strand, within wcaCat 5452.658 kb on - strand, within wcaC

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_JK32
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5,450,331 - +0.5
5,450,339 + +0.1
5,450,340 - -0.0
5,450,352 - +0.8
5,450,353 + +0.4
5,450,457 - -0.1
5,450,464 - -0.3
5,450,557 + wcaA JDDGAC_28425 0.11 +1.7
5,450,649 + wcaA JDDGAC_28425 0.22 +0.1
5,450,649 + wcaA JDDGAC_28425 0.22 -0.3
5,450,649 + wcaA JDDGAC_28425 0.22 -1.0
5,450,653 + wcaA JDDGAC_28425 0.23 -2.2
5,450,654 - wcaA JDDGAC_28425 0.23 -0.2
5,450,695 - wcaA JDDGAC_28425 0.28 +0.4
5,450,725 + wcaA JDDGAC_28425 0.31 +0.2
5,450,726 - wcaA JDDGAC_28425 0.31 +0.2
5,450,834 - wcaA JDDGAC_28425 0.44 -1.5
5,450,873 + wcaA JDDGAC_28425 0.49 -0.3
5,450,873 + wcaA JDDGAC_28425 0.49 +0.3
5,450,874 - wcaA JDDGAC_28425 0.49 -0.1
5,450,926 - wcaA JDDGAC_28425 0.55 +0.1
5,450,926 - wcaA JDDGAC_28425 0.55 +0.4
5,451,007 + wcaA JDDGAC_28425 0.65 +0.3
5,451,133 + wcaA JDDGAC_28425 0.80 -0.8
5,451,133 + wcaA JDDGAC_28425 0.80 +0.6
5,451,134 - wcaA JDDGAC_28425 0.80 +0.5
5,451,306 - +0.4
5,451,516 - wcaB JDDGAC_28430 0.43 -0.4
5,451,548 + wcaB JDDGAC_28430 0.49 -0.7
5,451,549 - wcaB JDDGAC_28430 0.50 +0.4
5,451,808 + -1.7
5,451,964 + wcaC JDDGAC_28435 0.14 +1.2
5,451,991 + wcaC JDDGAC_28435 0.16 -0.6
5,452,045 - wcaC JDDGAC_28435 0.21 +0.4
5,452,047 - wcaC JDDGAC_28435 0.21 -0.5
5,452,114 - wcaC JDDGAC_28435 0.27 -0.5
5,452,279 + wcaC JDDGAC_28435 0.40 -0.1
5,452,279 + wcaC JDDGAC_28435 0.40 +0.3
5,452,279 + wcaC JDDGAC_28435 0.40 +0.0
5,452,280 - wcaC JDDGAC_28435 0.40 -1.5
5,452,280 - wcaC JDDGAC_28435 0.40 +0.1
5,452,283 + wcaC JDDGAC_28435 0.40 -0.3
5,452,438 + wcaC JDDGAC_28435 0.53 -0.5
5,452,444 + wcaC JDDGAC_28435 0.54 +0.3
5,452,562 - wcaC JDDGAC_28435 0.63 -0.7
5,452,616 - wcaC JDDGAC_28435 0.68 -0.4
5,452,616 - wcaC JDDGAC_28435 0.68 +0.5
5,452,616 - wcaC JDDGAC_28435 0.68 +0.3
5,452,658 - wcaC JDDGAC_28435 0.71 +0.5

Or see this region's nucleotide sequence