Experiment: WM_JK32
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemW and rdgB overlap by 8 nucleotides rdgB and yggU are separated by 7 nucleotides yggU and yggT overlap by 4 nucleotides yggT and yggS are separated by 17 nucleotides
JDDGAC_22865: hemW - radical SAM family heme chaperone HemW, at 4,378,064 to 4,379,200
hemW
JDDGAC_22870: rdgB - XTP/dITP diphosphatase, at 4,379,193 to 4,379,786
rdgB
JDDGAC_22875: yggU - DUF167 domain-containing protein, at 4,379,794 to 4,380,084
yggU
JDDGAC_22880: yggT - osmotic shock tolerance protein YggT, at 4,380,081 to 4,380,647
yggT
JDDGAC_22885: yggS - pyridoxal phosphate homeostasis protein, at 4,380,665 to 4,381,369
yggS
Position (kb) 4379
4380
4381 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 4378.816 kb on + strand, within hemW at 4378.840 kb on + strand, within hemW at 4378.841 kb on - strand, within hemW at 4378.863 kb on + strand, within hemW at 4378.913 kb on - strand, within hemW at 4379.043 kb on + strand, within hemW at 4379.043 kb on + strand, within hemW at 4379.085 kb on + strand, within hemW at 4379.172 kb on + strand at 4379.173 kb on - strand at 4379.202 kb on - strand at 4379.202 kb on - strand at 4379.206 kb on - strand at 4379.302 kb on - strand, within rdgB at 4379.355 kb on - strand, within rdgB at 4379.566 kb on - strand, within rdgB at 4379.599 kb on - strand, within rdgB at 4379.786 kb on + strand at 4379.786 kb on + strand at 4379.787 kb on - strand at 4379.787 kb on - strand at 4379.792 kb on - strand at 4379.795 kb on + strand at 4379.795 kb on + strand at 4379.799 kb on + strand at 4379.799 kb on + strand at 4379.800 kb on - strand at 4379.999 kb on - strand, within yggU at 4380.009 kb on + strand, within yggU at 4380.009 kb on + strand, within yggU at 4380.010 kb on - strand, within yggU at 4380.038 kb on + strand, within yggU at 4380.038 kb on + strand, within yggU at 4380.039 kb on - strand, within yggU at 4380.039 kb on - strand, within yggU at 4380.067 kb on - strand at 4380.085 kb on - strand at 4380.133 kb on - strand at 4380.214 kb on - strand, within yggT at 4380.266 kb on - strand, within yggT at 4380.673 kb on + strand at 4380.848 kb on + strand, within yggS at 4380.848 kb on + strand, within yggS at 4380.849 kb on - strand, within yggS at 4380.849 kb on - strand, within yggS at 4380.853 kb on - strand, within yggS at 4380.853 kb on - strand, within yggS at 4380.853 kb on - strand, within yggS at 4380.853 kb on - strand, within yggS at 4380.853 kb on - strand, within yggS at 4380.961 kb on + strand, within yggS at 4380.962 kb on - strand, within yggS at 4381.067 kb on - strand, within yggS
Per-strain Table
Position Strand Gene LocusTag Fraction WM_JK32 remove 4,378,816 + hemW JDDGAC_22865 0.66 -1.0 4,378,840 + hemW JDDGAC_22865 0.68 +0.9 4,378,841 - hemW JDDGAC_22865 0.68 -1.3 4,378,863 + hemW JDDGAC_22865 0.70 -1.0 4,378,913 - hemW JDDGAC_22865 0.75 -0.4 4,379,043 + hemW JDDGAC_22865 0.86 +0.0 4,379,043 + hemW JDDGAC_22865 0.86 -2.0 4,379,085 + hemW JDDGAC_22865 0.90 -0.4 4,379,172 + -1.8 4,379,173 - +0.0 4,379,202 - -0.4 4,379,202 - +0.1 4,379,206 - -0.4 4,379,302 - rdgB JDDGAC_22870 0.18 -0.1 4,379,355 - rdgB JDDGAC_22870 0.27 -0.9 4,379,566 - rdgB JDDGAC_22870 0.63 -0.4 4,379,599 - rdgB JDDGAC_22870 0.68 -0.1 4,379,786 + -0.9 4,379,786 + +0.9 4,379,787 - -0.5 4,379,787 - +0.1 4,379,792 - -0.9 4,379,795 + -2.6 4,379,795 + -0.7 4,379,799 + -0.3 4,379,799 + -2.0 4,379,800 - +0.1 4,379,999 - yggU JDDGAC_22875 0.70 +0.1 4,380,009 + yggU JDDGAC_22875 0.74 -1.0 4,380,009 + yggU JDDGAC_22875 0.74 -0.6 4,380,010 - yggU JDDGAC_22875 0.74 +0.5 4,380,038 + yggU JDDGAC_22875 0.84 +0.4 4,380,038 + yggU JDDGAC_22875 0.84 +0.4 4,380,039 - yggU JDDGAC_22875 0.84 -0.3 4,380,039 - yggU JDDGAC_22875 0.84 +0.7 4,380,067 - -0.6 4,380,085 - +0.7 4,380,133 - -0.3 4,380,214 - yggT JDDGAC_22880 0.23 +0.1 4,380,266 - yggT JDDGAC_22880 0.33 -2.8 4,380,673 + -0.4 4,380,848 + yggS JDDGAC_22885 0.26 -2.8 4,380,848 + yggS JDDGAC_22885 0.26 -3.9 4,380,849 - yggS JDDGAC_22885 0.26 -2.9 4,380,849 - yggS JDDGAC_22885 0.26 -3.7 4,380,853 - yggS JDDGAC_22885 0.27 -3.6 4,380,853 - yggS JDDGAC_22885 0.27 -5.5 4,380,853 - yggS JDDGAC_22885 0.27 -4.3 4,380,853 - yggS JDDGAC_22885 0.27 -4.6 4,380,853 - yggS JDDGAC_22885 0.27 -4.4 4,380,961 + yggS JDDGAC_22885 0.42 -3.2 4,380,962 - yggS JDDGAC_22885 0.42 -3.8 4,381,067 - yggS JDDGAC_22885 0.57 -4.3
Or see this region's nucleotide sequence