Experiment: WM_JK32
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ler and escE are separated by 14 nucleotides escE and cesAB are separated by 12 nucleotides cesAB and escK overlap by 4 nucleotides escK and escL overlap by 14 nucleotides escL and escR are separated by 4 nucleotides
JDDGAC_18890: ler - transcription regulator Ler, at 3,597,834 to 3,598,205
ler
JDDGAC_18895: escE - chaperone for EscF, at 3,598,220 to 3,598,438
escE
JDDGAC_18900: cesAB - chaperone for EspA and EspB, at 3,598,451 to 3,598,765
cesAB
JDDGAC_18905: escK - type III secretion system sorting platform component, at 3,598,762 to 3,599,361
escK
JDDGAC_18910: escL - negative regulator EscL, stator protein, at 3,599,348 to 3,600,001
escL
JDDGAC_18915: escR - Type III secretion systemminor export apparatus protein EscR, at 3,600,006 to 3,600,659
escR
Position (kb)
3598
3599
3600 Strain fitness (log2 ratio)
-2
-1
0
1 at 3597.871 kb on - strand at 3597.947 kb on - strand, within ler at 3597.947 kb on - strand, within ler at 3597.984 kb on - strand, within ler at 3598.479 kb on + strand at 3598.631 kb on + strand, within cesAB at 3598.789 kb on - strand at 3598.806 kb on + strand at 3599.358 kb on - strand at 3599.720 kb on - strand, within escL at 3600.124 kb on + strand, within escR at 3600.230 kb on - strand, within escR
Per-strain Table
Position Strand Gene LocusTag Fraction WM_JK32 remove 3,597,871 - -0.7 3,597,947 - ler JDDGAC_18890 0.30 -0.5 3,597,947 - ler JDDGAC_18890 0.30 +0.1 3,597,984 - ler JDDGAC_18890 0.40 -1.9 3,598,479 + +0.6 3,598,631 + cesAB JDDGAC_18900 0.57 +0.2 3,598,789 - -0.7 3,598,806 + +0.2 3,599,358 - -0.3 3,599,720 - escL JDDGAC_18910 0.57 -0.1 3,600,124 + escR JDDGAC_18915 0.18 -0.2 3,600,230 - escR JDDGAC_18915 0.34 -1.1
Or see this region's nucleotide sequence