Experiment: WM_JK32
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt coaA and birA are separated by 28 nucleotides birA and murB overlap by 4 nucleotides murB and 5S_rrna are separated by 136 nucleotides 5S_rrna and 23S_rrna are separated by 94 nucleotides
JDDGAC_17110: coaA - type I pantothenate kinase, at 3,233,957 to 3,234,883
coaA
JDDGAC_17115: birA - bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA, at 3,234,912 to 3,235,877
birA
JDDGAC_17120: murB - UDP-N-acetylmuramate dehydrogenase, at 3,235,874 to 3,236,902
murB
JDDGAC_17125: 5S_rrna - 5S ribosomal RNA, at 3,237,039 to 3,237,154
_rrna
JDDGAC_17130: 23S_rrna - 23S ribosomal RNA, at 3,237,249 to 3,240,153
_rrna
Position (kb) 3235
3236
3237 Strain fitness (log2 ratio) -1
0
1 at 3235.869 kb on - strand at 3236.932 kb on - strand at 3236.932 kb on - strand at 3236.932 kb on - strand at 3236.932 kb on - strand at 3236.937 kb on - strand at 3236.942 kb on - strand at 3236.963 kb on - strand at 3236.987 kb on + strand at 3236.987 kb on + strand at 3237.054 kb on - strand, within 5S_rrna
Per-strain Table
Position Strand Gene LocusTag Fraction WM_JK32 remove 3,235,869 - -0.1 3,236,932 - +0.7 3,236,932 - -0.3 3,236,932 - -0.7 3,236,932 - +0.1 3,236,937 - -0.4 3,236,942 - -0.2 3,236,963 - +0.3 3,236,987 + -0.6 3,236,987 + -0.3 3,237,054 - 5S_rrna JDDGAC_17125 0.13 -0.5
Or see this region's nucleotide sequence