Experiment: WM_JK32
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt renD and JDDGAC_06585 overlap by 4 nucleotides JDDGAC_06585 and JDDGAC_06590 overlap by 4 nucleotides JDDGAC_06590 and xis are separated by 103 nucleotides xis and int overlap by 11 nucleotides
JDDGAC_06580: renD - Putative protein RenD, at 1,147,659 to 1,147,961
renD
JDDGAC_06585: JDDGAC_06585 - Replication protein P, at 1,147,958 to 1,148,659
_06585
JDDGAC_06590: JDDGAC_06590 - replication protein, at 1,148,656 to 1,149,480
_06590
JDDGAC_06595: xis - Excisionase, at 1,149,584 to 1,149,820
xis
JDDGAC_06600: int - Integrase, at 1,149,810 to 1,150,952
int
Position (kb) 1148
1149
1150 Strain fitness (log2 ratio) -2
-1
0
1 at 1147.686 kb on - strand at 1147.686 kb on - strand at 1147.689 kb on + strand at 1147.793 kb on - strand, within renD at 1147.793 kb on - strand, within renD at 1147.811 kb on + strand, within renD at 1147.864 kb on - strand, within renD at 1147.920 kb on - strand, within renD at 1147.941 kb on - strand at 1148.108 kb on - strand, within JDDGAC_06585 at 1149.107 kb on - strand, within JDDGAC_06590 at 1149.482 kb on - strand at 1149.530 kb on + strand at 1149.570 kb on + strand at 1150.062 kb on - strand, within int at 1150.082 kb on + strand, within int at 1150.127 kb on - strand, within int at 1150.432 kb on + strand, within int
Per-strain Table
Position Strand Gene LocusTag Fraction WM_JK32 remove 1,147,686 - -2.0 1,147,686 - +0.2 1,147,689 + +0.5 1,147,793 - renD JDDGAC_06580 0.44 +0.2 1,147,793 - renD JDDGAC_06580 0.44 -1.2 1,147,811 + renD JDDGAC_06580 0.50 -0.1 1,147,864 - renD JDDGAC_06580 0.68 +1.0 1,147,920 - renD JDDGAC_06580 0.86 +0.4 1,147,941 - -0.1 1,148,108 - JDDGAC_06585 0.21 -0.5 1,149,107 - JDDGAC_06590 0.55 +0.9 1,149,482 - -2.6 1,149,530 + +1.4 1,149,570 + +0.1 1,150,062 - int JDDGAC_06600 0.22 -2.1 1,150,082 + int JDDGAC_06600 0.24 -1.1 1,150,127 - int JDDGAC_06600 0.28 -0.3 1,150,432 + int JDDGAC_06600 0.54 -0.2
Or see this region's nucleotide sequence