Experiment: WM_JK32
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdeD and sdaA are separated by 130 nucleotides sdaA and nudL are separated by 183 nucleotides nudL and pabB are separated by 3 nucleotides
JDDGAC_01120: pdeD - putative cyclic di-GMP phosphodiesterase PdeD, at 187,385 to 188,983
pdeD
JDDGAC_01125: sdaA - L-serine ammonia-lyase, at 189,114 to 190,478
sdaA
JDDGAC_01130: nudL - CoA pyrophosphatase, at 190,662 to 191,240
nudL
JDDGAC_01135: pabB - aminodeoxychorismate synthase component 1, at 191,244 to 192,605
pabB
Position (kb) 189
190
191 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 188.214 kb on + strand, within pdeD at 188.215 kb on - strand, within pdeD at 188.232 kb on - strand, within pdeD at 188.232 kb on - strand, within pdeD at 188.386 kb on + strand, within pdeD at 188.482 kb on + strand, within pdeD at 188.510 kb on - strand, within pdeD at 188.510 kb on - strand, within pdeD at 188.726 kb on + strand, within pdeD at 188.727 kb on - strand, within pdeD at 188.792 kb on + strand, within pdeD at 188.805 kb on - strand, within pdeD at 188.810 kb on - strand, within pdeD at 188.814 kb on - strand, within pdeD at 188.844 kb on - strand at 188.846 kb on + strand at 189.051 kb on + strand at 189.074 kb on + strand at 189.106 kb on - strand at 189.113 kb on - strand at 189.194 kb on - strand at 189.544 kb on + strand, within sdaA at 189.544 kb on + strand, within sdaA at 189.545 kb on - strand, within sdaA at 189.545 kb on - strand, within sdaA at 189.545 kb on - strand, within sdaA at 189.545 kb on - strand, within sdaA at 189.581 kb on - strand, within sdaA at 189.581 kb on - strand, within sdaA at 189.581 kb on - strand, within sdaA at 189.581 kb on - strand, within sdaA at 189.688 kb on - strand, within sdaA at 189.857 kb on - strand, within sdaA at 190.064 kb on - strand, within sdaA at 190.232 kb on + strand, within sdaA at 190.233 kb on - strand, within sdaA at 190.263 kb on - strand, within sdaA at 190.353 kb on - strand at 190.413 kb on - strand at 190.455 kb on - strand at 190.484 kb on + strand at 190.535 kb on - strand at 190.535 kb on - strand at 190.647 kb on + strand at 190.648 kb on - strand at 190.654 kb on - strand at 190.657 kb on + strand at 190.658 kb on - strand at 190.658 kb on - strand at 190.729 kb on + strand, within nudL at 190.730 kb on - strand, within nudL at 190.777 kb on + strand, within nudL at 190.777 kb on + strand, within nudL at 190.809 kb on + strand, within nudL at 190.810 kb on - strand, within nudL at 190.810 kb on - strand, within nudL at 190.920 kb on + strand, within nudL at 190.920 kb on + strand, within nudL at 190.972 kb on - strand, within nudL at 190.974 kb on - strand, within nudL at 190.974 kb on - strand, within nudL at 190.974 kb on - strand, within nudL at 191.263 kb on - strand at 191.266 kb on - strand at 191.271 kb on - strand at 191.271 kb on - strand at 191.349 kb on + strand at 191.350 kb on - strand at 191.385 kb on - strand, within pabB at 191.385 kb on - strand, within pabB at 191.394 kb on - strand, within pabB at 191.445 kb on - strand, within pabB
Per-strain Table
Position Strand Gene LocusTag Fraction WM_JK32 remove 188,214 + pdeD JDDGAC_01120 0.52 -0.6 188,215 - pdeD JDDGAC_01120 0.52 +1.6 188,232 - pdeD JDDGAC_01120 0.53 -0.4 188,232 - pdeD JDDGAC_01120 0.53 +0.2 188,386 + pdeD JDDGAC_01120 0.63 -0.2 188,482 + pdeD JDDGAC_01120 0.69 +0.3 188,510 - pdeD JDDGAC_01120 0.70 -0.8 188,510 - pdeD JDDGAC_01120 0.70 -0.0 188,726 + pdeD JDDGAC_01120 0.84 +1.2 188,727 - pdeD JDDGAC_01120 0.84 +0.6 188,792 + pdeD JDDGAC_01120 0.88 -1.8 188,805 - pdeD JDDGAC_01120 0.89 -0.5 188,810 - pdeD JDDGAC_01120 0.89 +0.0 188,814 - pdeD JDDGAC_01120 0.89 -0.7 188,844 - +0.4 188,846 + +0.5 189,051 + +0.2 189,074 + -2.3 189,106 - +0.2 189,113 - +0.3 189,194 - +0.0 189,544 + sdaA JDDGAC_01125 0.32 -2.5 189,544 + sdaA JDDGAC_01125 0.32 +0.0 189,545 - sdaA JDDGAC_01125 0.32 -0.1 189,545 - sdaA JDDGAC_01125 0.32 +0.0 189,545 - sdaA JDDGAC_01125 0.32 +0.0 189,545 - sdaA JDDGAC_01125 0.32 -1.1 189,581 - sdaA JDDGAC_01125 0.34 -0.6 189,581 - sdaA JDDGAC_01125 0.34 -0.4 189,581 - sdaA JDDGAC_01125 0.34 -1.2 189,581 - sdaA JDDGAC_01125 0.34 +0.2 189,688 - sdaA JDDGAC_01125 0.42 +0.0 189,857 - sdaA JDDGAC_01125 0.54 -0.9 190,064 - sdaA JDDGAC_01125 0.70 -0.4 190,232 + sdaA JDDGAC_01125 0.82 -0.8 190,233 - sdaA JDDGAC_01125 0.82 -0.1 190,263 - sdaA JDDGAC_01125 0.84 +0.4 190,353 - -1.4 190,413 - -0.1 190,455 - -4.5 190,484 + -3.1 190,535 - -0.1 190,535 - -0.5 190,647 + -0.3 190,648 - -0.4 190,654 - -2.2 190,657 + +0.0 190,658 - -0.6 190,658 - -0.7 190,729 + nudL JDDGAC_01130 0.12 +0.4 190,730 - nudL JDDGAC_01130 0.12 -0.1 190,777 + nudL JDDGAC_01130 0.20 +0.1 190,777 + nudL JDDGAC_01130 0.20 -1.4 190,809 + nudL JDDGAC_01130 0.25 -1.1 190,810 - nudL JDDGAC_01130 0.26 -0.4 190,810 - nudL JDDGAC_01130 0.26 +1.7 190,920 + nudL JDDGAC_01130 0.45 +0.3 190,920 + nudL JDDGAC_01130 0.45 +0.0 190,972 - nudL JDDGAC_01130 0.54 -0.8 190,974 - nudL JDDGAC_01130 0.54 -2.4 190,974 - nudL JDDGAC_01130 0.54 -0.5 190,974 - nudL JDDGAC_01130 0.54 -0.7 191,263 - -0.8 191,266 - -1.1 191,271 - +0.9 191,271 - -0.8 191,349 + +0.4 191,350 - +0.2 191,385 - pabB JDDGAC_01135 0.10 +1.3 191,385 - pabB JDDGAC_01135 0.10 -0.6 191,394 - pabB JDDGAC_01135 0.11 +0.1 191,445 - pabB JDDGAC_01135 0.15 -0.7
Or see this region's nucleotide sequence