Experiment: WM_JK32
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfcG and yfcF are separated by 135 nucleotides yfcF and yfcE are separated by 55 nucleotides yfcE and yfcD are separated by 57 nucleotides yfcD and yfcC are separated by 32 nucleotides
JDDGAC_26730: yfcG - GSH-dependent disulfide bond oxidoreductase, at 5,114,832 to 5,115,479
yfcG
JDDGAC_26735: yfcF - glutathione transferase, at 5,115,615 to 5,116,259
yfcF
JDDGAC_26740: yfcE - phosphodiesterase, at 5,116,315 to 5,116,866
yfcE
JDDGAC_26745: yfcD - NUDIX hydrolase YfcD, at 5,116,924 to 5,117,466
yfcD
JDDGAC_26750: yfcC - putative basic amino acid antiporter YfcC, at 5,117,499 to 5,118,977
yfcC
Position (kb)
5116
5117 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5115.387 kb on + strand, within yfcG at 5115.388 kb on - strand, within yfcG at 5115.388 kb on - strand, within yfcG at 5115.393 kb on + strand, within yfcG at 5115.434 kb on + strand at 5115.517 kb on + strand at 5115.517 kb on + strand at 5115.536 kb on + strand at 5115.536 kb on + strand at 5115.575 kb on + strand at 5115.577 kb on + strand at 5115.577 kb on + strand at 5115.577 kb on + strand at 5115.577 kb on + strand at 5115.578 kb on - strand at 5115.578 kb on - strand at 5115.600 kb on - strand at 5115.613 kb on + strand at 5115.613 kb on + strand at 5115.613 kb on + strand at 5115.614 kb on - strand at 5115.667 kb on - strand at 5115.772 kb on - strand, within yfcF at 5115.885 kb on - strand, within yfcF at 5115.885 kb on - strand, within yfcF at 5115.885 kb on - strand, within yfcF at 5116.072 kb on + strand, within yfcF at 5116.073 kb on - strand, within yfcF at 5116.153 kb on + strand, within yfcF at 5116.153 kb on + strand, within yfcF at 5116.153 kb on + strand, within yfcF at 5116.153 kb on + strand, within yfcF at 5116.154 kb on - strand, within yfcF at 5116.188 kb on + strand, within yfcF at 5116.230 kb on - strand at 5116.284 kb on + strand at 5116.284 kb on + strand at 5116.284 kb on + strand at 5116.285 kb on - strand at 5116.285 kb on - strand at 5116.285 kb on - strand at 5116.290 kb on - strand at 5116.506 kb on + strand, within yfcE at 5116.506 kb on + strand, within yfcE at 5116.506 kb on + strand, within yfcE at 5116.506 kb on + strand, within yfcE at 5116.506 kb on + strand, within yfcE at 5116.507 kb on - strand, within yfcE at 5116.507 kb on - strand, within yfcE at 5116.507 kb on - strand, within yfcE at 5116.507 kb on - strand, within yfcE at 5116.592 kb on + strand, within yfcE at 5116.592 kb on + strand, within yfcE at 5116.592 kb on + strand, within yfcE at 5116.685 kb on - strand, within yfcE at 5116.783 kb on + strand, within yfcE at 5116.783 kb on + strand, within yfcE at 5116.784 kb on - strand, within yfcE at 5116.805 kb on + strand, within yfcE at 5116.805 kb on + strand, within yfcE at 5116.805 kb on + strand, within yfcE at 5116.806 kb on - strand, within yfcE at 5116.809 kb on + strand, within yfcE at 5116.809 kb on + strand, within yfcE at 5116.810 kb on - strand, within yfcE at 5116.810 kb on - strand, within yfcE at 5116.810 kb on - strand, within yfcE at 5116.837 kb on - strand at 5116.947 kb on + strand at 5117.044 kb on + strand, within yfcD at 5117.044 kb on + strand, within yfcD at 5117.144 kb on + strand, within yfcD at 5117.491 kb on + strand at 5117.547 kb on - strand at 5117.549 kb on + strand at 5117.549 kb on + strand at 5117.549 kb on + strand at 5117.549 kb on + strand at 5117.591 kb on + strand at 5117.592 kb on - strand at 5117.811 kb on - strand, within yfcC
Per-strain Table
Position Strand Gene LocusTag Fraction WM_JK32 remove 5,115,387 + yfcG JDDGAC_26730 0.86 +0.9 5,115,388 - yfcG JDDGAC_26730 0.86 +0.2 5,115,388 - yfcG JDDGAC_26730 0.86 -0.2 5,115,393 + yfcG JDDGAC_26730 0.87 -1.0 5,115,434 + -0.4 5,115,517 + +0.6 5,115,517 + +0.5 5,115,536 + -0.0 5,115,536 + -1.1 5,115,575 + +0.4 5,115,577 + +0.3 5,115,577 + -0.9 5,115,577 + -0.3 5,115,577 + +0.1 5,115,578 - +1.1 5,115,578 - +1.1 5,115,600 - +0.7 5,115,613 + -0.3 5,115,613 + -1.5 5,115,613 + +0.1 5,115,614 - +0.1 5,115,667 - -0.4 5,115,772 - yfcF JDDGAC_26735 0.24 +0.4 5,115,885 - yfcF JDDGAC_26735 0.42 -1.3 5,115,885 - yfcF JDDGAC_26735 0.42 +1.5 5,115,885 - yfcF JDDGAC_26735 0.42 +0.1 5,116,072 + yfcF JDDGAC_26735 0.71 +0.0 5,116,073 - yfcF JDDGAC_26735 0.71 +0.3 5,116,153 + yfcF JDDGAC_26735 0.83 -0.5 5,116,153 + yfcF JDDGAC_26735 0.83 +0.0 5,116,153 + yfcF JDDGAC_26735 0.83 -0.6 5,116,153 + yfcF JDDGAC_26735 0.83 +0.3 5,116,154 - yfcF JDDGAC_26735 0.84 -1.6 5,116,188 + yfcF JDDGAC_26735 0.89 +0.4 5,116,230 - +1.1 5,116,284 + +0.4 5,116,284 + +1.4 5,116,284 + -0.5 5,116,285 - -0.1 5,116,285 - +0.7 5,116,285 - -0.7 5,116,290 - -1.8 5,116,506 + yfcE JDDGAC_26740 0.35 +0.9 5,116,506 + yfcE JDDGAC_26740 0.35 -0.1 5,116,506 + yfcE JDDGAC_26740 0.35 -1.0 5,116,506 + yfcE JDDGAC_26740 0.35 -0.3 5,116,506 + yfcE JDDGAC_26740 0.35 +1.3 5,116,507 - yfcE JDDGAC_26740 0.35 -0.5 5,116,507 - yfcE JDDGAC_26740 0.35 +1.1 5,116,507 - yfcE JDDGAC_26740 0.35 +0.2 5,116,507 - yfcE JDDGAC_26740 0.35 -0.7 5,116,592 + yfcE JDDGAC_26740 0.50 +0.2 5,116,592 + yfcE JDDGAC_26740 0.50 +0.5 5,116,592 + yfcE JDDGAC_26740 0.50 +0.9 5,116,685 - yfcE JDDGAC_26740 0.67 -0.2 5,116,783 + yfcE JDDGAC_26740 0.85 +0.3 5,116,783 + yfcE JDDGAC_26740 0.85 -0.1 5,116,784 - yfcE JDDGAC_26740 0.85 +0.7 5,116,805 + yfcE JDDGAC_26740 0.89 -0.6 5,116,805 + yfcE JDDGAC_26740 0.89 -1.0 5,116,805 + yfcE JDDGAC_26740 0.89 +0.9 5,116,806 - yfcE JDDGAC_26740 0.89 +1.0 5,116,809 + yfcE JDDGAC_26740 0.89 -0.7 5,116,809 + yfcE JDDGAC_26740 0.89 +0.2 5,116,810 - yfcE JDDGAC_26740 0.90 +0.3 5,116,810 - yfcE JDDGAC_26740 0.90 -0.3 5,116,810 - yfcE JDDGAC_26740 0.90 -0.1 5,116,837 - +1.2 5,116,947 + +0.1 5,117,044 + yfcD JDDGAC_26745 0.22 +1.1 5,117,044 + yfcD JDDGAC_26745 0.22 -3.0 5,117,144 + yfcD JDDGAC_26745 0.41 -0.5 5,117,491 + +0.0 5,117,547 - +1.0 5,117,549 + +0.4 5,117,549 + +0.5 5,117,549 + -0.3 5,117,549 + -0.2 5,117,591 + +0.1 5,117,592 - +0.5 5,117,811 - yfcC JDDGAC_26750 0.21 +1.1
Or see this region's nucleotide sequence