Experiment: WM_K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wzxC and wcaK are separated by 179 nucleotides wcaK and wcaL overlap by 4 nucleotides
JDDGAC_28490: wzxC - colanic acid undecaprenyl disphosphate flippase WzxC, at 5,463,794 to 5,465,272
wzxC
JDDGAC_28495: wcaK - colanic acid biosynthesis pyruvyl transferase WcaK, at 5,465,452 to 5,466,732
wcaK
JDDGAC_28500: wcaL - colanic acid biosynthesis glycosyltransferase WcaL, at 5,466,729 to 5,467,949
wcaL
Position (kb)
5465
5466
5467 Strain fitness (log2 ratio)
-2
-1
0
1 at 5464.464 kb on - strand, within wzxC at 5464.530 kb on - strand, within wzxC at 5464.810 kb on - strand, within wzxC at 5464.969 kb on + strand, within wzxC at 5465.034 kb on - strand, within wzxC at 5465.090 kb on + strand, within wzxC at 5465.090 kb on + strand, within wzxC at 5465.091 kb on - strand, within wzxC at 5465.151 kb on + strand at 5465.314 kb on + strand at 5465.324 kb on + strand at 5465.365 kb on + strand at 5465.460 kb on - strand at 5465.464 kb on - strand at 5465.520 kb on - strand at 5465.551 kb on - strand at 5465.714 kb on + strand, within wcaK at 5465.715 kb on - strand, within wcaK at 5465.715 kb on - strand, within wcaK at 5465.815 kb on - strand, within wcaK at 5465.815 kb on - strand, within wcaK at 5465.886 kb on + strand, within wcaK at 5466.115 kb on + strand, within wcaK at 5466.169 kb on - strand, within wcaK at 5466.223 kb on + strand, within wcaK at 5466.313 kb on + strand, within wcaK at 5466.314 kb on - strand, within wcaK at 5466.315 kb on + strand, within wcaK at 5466.316 kb on - strand, within wcaK at 5466.384 kb on - strand, within wcaK at 5466.511 kb on + strand, within wcaK at 5466.512 kb on - strand, within wcaK at 5466.519 kb on + strand, within wcaK at 5466.520 kb on - strand, within wcaK at 5466.652 kb on - strand at 5466.878 kb on + strand, within wcaL at 5466.879 kb on - strand, within wcaL at 5466.879 kb on - strand, within wcaL at 5467.219 kb on - strand, within wcaL at 5467.345 kb on + strand, within wcaL at 5467.346 kb on - strand, within wcaL at 5467.346 kb on - strand, within wcaL at 5467.349 kb on + strand, within wcaL at 5467.350 kb on - strand, within wcaL at 5467.551 kb on + strand, within wcaL
Per-strain Table
Position Strand Gene LocusTag Fraction WM_K30 remove 5,464,464 - wzxC JDDGAC_28490 0.45 -0.8 5,464,530 - wzxC JDDGAC_28490 0.50 -0.5 5,464,810 - wzxC JDDGAC_28490 0.69 +0.2 5,464,969 + wzxC JDDGAC_28490 0.79 -0.7 5,465,034 - wzxC JDDGAC_28490 0.84 +1.0 5,465,090 + wzxC JDDGAC_28490 0.88 +0.7 5,465,090 + wzxC JDDGAC_28490 0.88 +0.7 5,465,091 - wzxC JDDGAC_28490 0.88 +1.2 5,465,151 + -2.5 5,465,314 + +0.6 5,465,324 + -0.5 5,465,365 + +1.4 5,465,460 - +0.2 5,465,464 - +1.0 5,465,520 - -0.1 5,465,551 - +0.0 5,465,714 + wcaK JDDGAC_28495 0.20 +0.4 5,465,715 - wcaK JDDGAC_28495 0.21 -0.5 5,465,715 - wcaK JDDGAC_28495 0.21 -0.5 5,465,815 - wcaK JDDGAC_28495 0.28 -0.3 5,465,815 - wcaK JDDGAC_28495 0.28 +0.7 5,465,886 + wcaK JDDGAC_28495 0.34 +0.2 5,466,115 + wcaK JDDGAC_28495 0.52 +0.5 5,466,169 - wcaK JDDGAC_28495 0.56 -0.1 5,466,223 + wcaK JDDGAC_28495 0.60 -1.2 5,466,313 + wcaK JDDGAC_28495 0.67 -0.2 5,466,314 - wcaK JDDGAC_28495 0.67 -0.2 5,466,315 + wcaK JDDGAC_28495 0.67 +0.7 5,466,316 - wcaK JDDGAC_28495 0.67 -0.2 5,466,384 - wcaK JDDGAC_28495 0.73 +0.6 5,466,511 + wcaK JDDGAC_28495 0.83 -0.5 5,466,512 - wcaK JDDGAC_28495 0.83 +0.4 5,466,519 + wcaK JDDGAC_28495 0.83 +0.2 5,466,520 - wcaK JDDGAC_28495 0.83 -0.3 5,466,652 - +0.7 5,466,878 + wcaL JDDGAC_28500 0.12 -0.1 5,466,879 - wcaL JDDGAC_28500 0.12 -1.5 5,466,879 - wcaL JDDGAC_28500 0.12 +0.5 5,467,219 - wcaL JDDGAC_28500 0.40 +0.6 5,467,345 + wcaL JDDGAC_28500 0.50 -0.3 5,467,346 - wcaL JDDGAC_28500 0.51 -1.3 5,467,346 - wcaL JDDGAC_28500 0.51 -1.0 5,467,349 + wcaL JDDGAC_28500 0.51 +1.3 5,467,350 - wcaL JDDGAC_28500 0.51 -0.7 5,467,551 + wcaL JDDGAC_28500 0.67 -0.5
Or see this region's nucleotide sequence