Strain Fitness in Escherichia coli ECRC98 around JDDGAC_28360

Experiment: WM_K30

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyegI and yegD are separated by 12 nucleotidesyegD and alkA are separated by 181 nucleotides JDDGAC_28355: yegI - protein kinase YegI, at 5,432,417 to 5,434,357 yegI JDDGAC_28360: yegD - molecular chaperone, at 5,434,370 to 5,435,674 yegD JDDGAC_28365: alkA - DNA-3-methyladenine glycosylase 2, at 5,435,856 to 5,436,704 alkA Position (kb) 5434 5435 5436Strain fitness (log2 ratio) -1 0 1at 5433.416 kb on + strand, within yegIat 5433.568 kb on - strand, within yegIat 5433.588 kb on - strand, within yegIat 5433.660 kb on - strand, within yegIat 5433.660 kb on - strand, within yegIat 5433.664 kb on + strand, within yegIat 5433.758 kb on - strand, within yegIat 5433.776 kb on + strand, within yegIat 5433.776 kb on + strand, within yegIat 5433.827 kb on - strand, within yegIat 5434.000 kb on + strand, within yegIat 5434.512 kb on + strand, within yegDat 5434.513 kb on - strand, within yegDat 5434.517 kb on - strand, within yegDat 5434.517 kb on - strand, within yegDat 5434.724 kb on + strand, within yegDat 5434.784 kb on + strand, within yegDat 5434.841 kb on + strand, within yegDat 5434.841 kb on + strand, within yegDat 5434.842 kb on - strand, within yegDat 5435.108 kb on + strand, within yegDat 5435.109 kb on - strand, within yegDat 5435.122 kb on - strand, within yegDat 5435.436 kb on + strand, within yegDat 5435.436 kb on + strand, within yegDat 5435.437 kb on - strand, within yegDat 5435.489 kb on - strand, within yegDat 5435.504 kb on - strand, within yegDat 5435.699 kb on - strandat 5435.859 kb on + strandat 5435.860 kb on - strandat 5435.958 kb on + strand, within alkAat 5435.967 kb on - strand, within alkAat 5436.016 kb on + strand, within alkAat 5436.017 kb on - strand, within alkAat 5436.026 kb on + strand, within alkAat 5436.043 kb on + strand, within alkAat 5436.309 kb on + strand, within alkAat 5436.310 kb on - strand, within alkAat 5436.310 kb on - strand, within alkAat 5436.311 kb on + strand, within alkAat 5436.371 kb on - strand, within alkAat 5436.374 kb on + strand, within alkAat 5436.375 kb on - strand, within alkAat 5436.375 kb on - strand, within alkAat 5436.519 kb on + strand, within alkAat 5436.570 kb on + strand, within alkAat 5436.570 kb on + strand, within alkAat 5436.651 kb on + strandat 5436.651 kb on + strandat 5436.665 kb on + strandat 5436.665 kb on + strandat 5436.666 kb on - strandat 5436.673 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_K30
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5,433,416 + yegI JDDGAC_28355 0.51 +1.4
5,433,568 - yegI JDDGAC_28355 0.59 +0.1
5,433,588 - yegI JDDGAC_28355 0.60 -0.2
5,433,660 - yegI JDDGAC_28355 0.64 +0.1
5,433,660 - yegI JDDGAC_28355 0.64 -0.4
5,433,664 + yegI JDDGAC_28355 0.64 +0.5
5,433,758 - yegI JDDGAC_28355 0.69 +0.9
5,433,776 + yegI JDDGAC_28355 0.70 -0.4
5,433,776 + yegI JDDGAC_28355 0.70 -1.2
5,433,827 - yegI JDDGAC_28355 0.73 -1.4
5,434,000 + yegI JDDGAC_28355 0.82 -0.2
5,434,512 + yegD JDDGAC_28360 0.11 +1.4
5,434,513 - yegD JDDGAC_28360 0.11 +1.0
5,434,517 - yegD JDDGAC_28360 0.11 -0.7
5,434,517 - yegD JDDGAC_28360 0.11 +0.5
5,434,724 + yegD JDDGAC_28360 0.27 -0.8
5,434,784 + yegD JDDGAC_28360 0.32 -0.1
5,434,841 + yegD JDDGAC_28360 0.36 +0.6
5,434,841 + yegD JDDGAC_28360 0.36 -0.9
5,434,842 - yegD JDDGAC_28360 0.36 -1.1
5,435,108 + yegD JDDGAC_28360 0.57 -0.6
5,435,109 - yegD JDDGAC_28360 0.57 -1.4
5,435,122 - yegD JDDGAC_28360 0.58 -0.8
5,435,436 + yegD JDDGAC_28360 0.82 -0.1
5,435,436 + yegD JDDGAC_28360 0.82 +1.2
5,435,437 - yegD JDDGAC_28360 0.82 +0.1
5,435,489 - yegD JDDGAC_28360 0.86 +0.3
5,435,504 - yegD JDDGAC_28360 0.87 -0.1
5,435,699 - +0.0
5,435,859 + +0.0
5,435,860 - -1.6
5,435,958 + alkA JDDGAC_28365 0.12 -0.4
5,435,967 - alkA JDDGAC_28365 0.13 -0.9
5,436,016 + alkA JDDGAC_28365 0.19 +0.7
5,436,017 - alkA JDDGAC_28365 0.19 -0.2
5,436,026 + alkA JDDGAC_28365 0.20 -0.7
5,436,043 + alkA JDDGAC_28365 0.22 -0.1
5,436,309 + alkA JDDGAC_28365 0.53 +1.7
5,436,310 - alkA JDDGAC_28365 0.53 -0.1
5,436,310 - alkA JDDGAC_28365 0.53 -1.0
5,436,311 + alkA JDDGAC_28365 0.54 +0.4
5,436,371 - alkA JDDGAC_28365 0.61 -0.2
5,436,374 + alkA JDDGAC_28365 0.61 +0.7
5,436,375 - alkA JDDGAC_28365 0.61 +0.2
5,436,375 - alkA JDDGAC_28365 0.61 +1.0
5,436,519 + alkA JDDGAC_28365 0.78 +0.2
5,436,570 + alkA JDDGAC_28365 0.84 +0.9
5,436,570 + alkA JDDGAC_28365 0.84 +0.4
5,436,651 + -0.1
5,436,651 + -0.6
5,436,665 + -0.7
5,436,665 + -0.2
5,436,666 - -1.1
5,436,673 - +1.7

Or see this region's nucleotide sequence