Experiment: WM_K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt infB and rbfA are separated by 164 nucleotides rbfA and truB overlap by 1 nucleotides truB and rpsO are separated by 148 nucleotides rpsO and pnp are separated by 246 nucleotides
JDDGAC_21735: infB - Translation initiation factor IF-2, at 4,159,571 to 4,162,243
infB
JDDGAC_21745: rbfA - 30S ribosome-binding factor RbfA, at 4,162,408 to 4,162,809
rbfA
JDDGAC_21750: truB - tRNA pseudouridine(55) synthase TruB, at 4,162,809 to 4,163,753
truB
JDDGAC_21755: rpsO - 30S ribosomal protein S15, at 4,163,902 to 4,164,171
rpsO
JDDGAC_21765: pnp - polyribonucleotide nucleotidyltransferase, at 4,164,418 to 4,166,553
pnp
Position (kb) 4162
4163
4164 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4162.231 kb on + strand at 4162.231 kb on + strand at 4162.732 kb on - strand, within rbfA at 4162.732 kb on - strand, within rbfA at 4162.807 kb on + strand at 4162.874 kb on + strand at 4162.910 kb on + strand, within truB at 4162.911 kb on - strand, within truB at 4162.912 kb on + strand, within truB at 4162.912 kb on + strand, within truB at 4162.912 kb on + strand, within truB at 4162.913 kb on - strand, within truB at 4162.914 kb on + strand, within truB at 4162.914 kb on + strand, within truB at 4162.914 kb on + strand, within truB at 4162.915 kb on - strand, within truB at 4162.915 kb on - strand, within truB at 4162.915 kb on - strand, within truB at 4162.915 kb on - strand, within truB at 4162.915 kb on - strand, within truB at 4162.916 kb on + strand, within truB at 4162.916 kb on + strand, within truB at 4162.916 kb on + strand, within truB at 4162.917 kb on - strand, within truB at 4162.917 kb on - strand, within truB at 4163.064 kb on - strand, within truB at 4163.064 kb on - strand, within truB at 4163.069 kb on + strand, within truB at 4163.069 kb on + strand, within truB at 4163.148 kb on - strand, within truB at 4163.184 kb on + strand, within truB at 4163.199 kb on + strand, within truB at 4163.200 kb on - strand, within truB at 4163.223 kb on + strand, within truB at 4163.224 kb on - strand, within truB at 4163.343 kb on + strand, within truB at 4163.343 kb on + strand, within truB at 4163.343 kb on + strand, within truB at 4163.344 kb on - strand, within truB at 4163.344 kb on - strand, within truB at 4163.344 kb on - strand, within truB at 4163.344 kb on - strand, within truB at 4163.344 kb on - strand, within truB at 4163.344 kb on - strand, within truB at 4163.345 kb on + strand, within truB at 4163.346 kb on - strand, within truB at 4163.621 kb on + strand, within truB at 4163.751 kb on + strand at 4163.757 kb on + strand at 4163.839 kb on + strand at 4164.245 kb on + strand at 4164.250 kb on + strand at 4164.250 kb on + strand at 4164.251 kb on - strand at 4164.259 kb on + strand at 4164.369 kb on + strand at 4164.383 kb on + strand at 4164.388 kb on + strand at 4164.414 kb on + strand at 4164.517 kb on - strand at 4164.544 kb on - strand at 4164.600 kb on + strand at 4164.604 kb on + strand at 4164.605 kb on - strand at 4164.616 kb on - strand at 4164.616 kb on - strand at 4164.620 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_K30 remove 4,162,231 + -2.3 4,162,231 + -0.8 4,162,732 - rbfA JDDGAC_21745 0.81 -1.3 4,162,732 - rbfA JDDGAC_21745 0.81 -0.5 4,162,807 + -0.5 4,162,874 + -0.5 4,162,910 + truB JDDGAC_21750 0.11 -0.7 4,162,911 - truB JDDGAC_21750 0.11 +0.1 4,162,912 + truB JDDGAC_21750 0.11 -1.0 4,162,912 + truB JDDGAC_21750 0.11 +0.5 4,162,912 + truB JDDGAC_21750 0.11 +2.5 4,162,913 - truB JDDGAC_21750 0.11 -0.8 4,162,914 + truB JDDGAC_21750 0.11 +0.2 4,162,914 + truB JDDGAC_21750 0.11 +1.1 4,162,914 + truB JDDGAC_21750 0.11 +0.4 4,162,915 - truB JDDGAC_21750 0.11 -1.1 4,162,915 - truB JDDGAC_21750 0.11 +1.0 4,162,915 - truB JDDGAC_21750 0.11 -1.8 4,162,915 - truB JDDGAC_21750 0.11 +0.7 4,162,915 - truB JDDGAC_21750 0.11 -0.2 4,162,916 + truB JDDGAC_21750 0.11 +0.4 4,162,916 + truB JDDGAC_21750 0.11 +0.1 4,162,916 + truB JDDGAC_21750 0.11 -1.1 4,162,917 - truB JDDGAC_21750 0.11 +1.0 4,162,917 - truB JDDGAC_21750 0.11 +0.6 4,163,064 - truB JDDGAC_21750 0.27 -0.9 4,163,064 - truB JDDGAC_21750 0.27 +0.3 4,163,069 + truB JDDGAC_21750 0.28 +0.0 4,163,069 + truB JDDGAC_21750 0.28 -0.2 4,163,148 - truB JDDGAC_21750 0.36 -0.7 4,163,184 + truB JDDGAC_21750 0.40 +0.1 4,163,199 + truB JDDGAC_21750 0.41 -0.4 4,163,200 - truB JDDGAC_21750 0.41 -0.1 4,163,223 + truB JDDGAC_21750 0.44 -0.4 4,163,224 - truB JDDGAC_21750 0.44 +0.3 4,163,343 + truB JDDGAC_21750 0.57 -0.6 4,163,343 + truB JDDGAC_21750 0.57 +0.5 4,163,343 + truB JDDGAC_21750 0.57 -0.4 4,163,344 - truB JDDGAC_21750 0.57 -1.4 4,163,344 - truB JDDGAC_21750 0.57 -0.5 4,163,344 - truB JDDGAC_21750 0.57 -0.8 4,163,344 - truB JDDGAC_21750 0.57 -0.1 4,163,344 - truB JDDGAC_21750 0.57 -1.3 4,163,344 - truB JDDGAC_21750 0.57 -0.2 4,163,345 + truB JDDGAC_21750 0.57 -1.0 4,163,346 - truB JDDGAC_21750 0.57 -0.9 4,163,621 + truB JDDGAC_21750 0.86 -0.5 4,163,751 + +0.2 4,163,757 + +0.2 4,163,839 + -2.3 4,164,245 + -1.2 4,164,250 + +0.4 4,164,250 + +0.7 4,164,251 - -2.3 4,164,259 + +0.4 4,164,369 + +0.3 4,164,383 + +0.2 4,164,388 + -0.2 4,164,414 + -1.1 4,164,517 - -0.4 4,164,544 - -0.4 4,164,600 + +0.3 4,164,604 + +1.9 4,164,605 - -2.0 4,164,616 - +1.0 4,164,616 - +0.3 4,164,620 - -1.4
Or see this region's nucleotide sequence