Experiment: WM_K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfaJ and rfaB are separated by 8 nucleotides rfaB and rfaL are separated by 35 nucleotides
JDDGAC_19300: rfaJ - glucosyltransferase, at 3,670,800 to 3,671,813
rfaJ
JDDGAC_19305: rfaB - UDP-glucose--(glucosyl)LPS alpha-1,2-glucosyltransferase, at 3,671,822 to 3,672,964
rfaB
JDDGAC_19310: rfaL - O-antigen ligase RfaL, at 3,673,000 to 3,674,208
rfaL
Position (kb)
3671
3672
3673 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3670.984 kb on + strand, within rfaJ at 3671.010 kb on + strand, within rfaJ at 3671.179 kb on + strand, within rfaJ at 3671.310 kb on - strand, within rfaJ at 3671.513 kb on + strand, within rfaJ at 3671.593 kb on + strand, within rfaJ at 3671.598 kb on + strand, within rfaJ at 3671.637 kb on + strand, within rfaJ at 3671.904 kb on - strand at 3672.072 kb on - strand, within rfaB at 3672.198 kb on + strand, within rfaB at 3672.386 kb on - strand, within rfaB at 3672.601 kb on + strand, within rfaB at 3672.601 kb on + strand, within rfaB at 3672.601 kb on + strand, within rfaB at 3672.602 kb on - strand, within rfaB at 3672.607 kb on - strand, within rfaB at 3672.712 kb on + strand, within rfaB at 3672.779 kb on - strand, within rfaB at 3672.806 kb on - strand, within rfaB at 3672.905 kb on + strand at 3672.962 kb on + strand at 3673.189 kb on + strand, within rfaL at 3673.216 kb on + strand, within rfaL at 3673.261 kb on + strand, within rfaL at 3673.272 kb on + strand, within rfaL at 3673.305 kb on + strand, within rfaL at 3673.323 kb on - strand, within rfaL at 3673.437 kb on - strand, within rfaL
Per-strain Table
Position Strand Gene LocusTag Fraction WM_K30 remove 3,670,984 + rfaJ JDDGAC_19300 0.18 -1.2 3,671,010 + rfaJ JDDGAC_19300 0.21 -5.7 3,671,179 + rfaJ JDDGAC_19300 0.37 -3.5 3,671,310 - rfaJ JDDGAC_19300 0.50 -4.4 3,671,513 + rfaJ JDDGAC_19300 0.70 -3.3 3,671,593 + rfaJ JDDGAC_19300 0.78 -3.5 3,671,598 + rfaJ JDDGAC_19300 0.79 -2.8 3,671,637 + rfaJ JDDGAC_19300 0.83 -4.4 3,671,904 - -2.1 3,672,072 - rfaB JDDGAC_19305 0.22 -1.9 3,672,198 + rfaB JDDGAC_19305 0.33 -3.3 3,672,386 - rfaB JDDGAC_19305 0.49 -3.6 3,672,601 + rfaB JDDGAC_19305 0.68 -3.6 3,672,601 + rfaB JDDGAC_19305 0.68 -3.1 3,672,601 + rfaB JDDGAC_19305 0.68 -5.7 3,672,602 - rfaB JDDGAC_19305 0.68 -3.9 3,672,607 - rfaB JDDGAC_19305 0.69 -4.1 3,672,712 + rfaB JDDGAC_19305 0.78 -2.8 3,672,779 - rfaB JDDGAC_19305 0.84 -1.0 3,672,806 - rfaB JDDGAC_19305 0.86 -4.8 3,672,905 + -2.4 3,672,962 + +0.6 3,673,189 + rfaL JDDGAC_19310 0.16 -2.9 3,673,216 + rfaL JDDGAC_19310 0.18 -5.1 3,673,261 + rfaL JDDGAC_19310 0.22 -3.1 3,673,272 + rfaL JDDGAC_19310 0.22 -4.3 3,673,305 + rfaL JDDGAC_19310 0.25 -3.3 3,673,323 - rfaL JDDGAC_19310 0.27 -3.2 3,673,437 - rfaL JDDGAC_19310 0.36 -5.8
Or see this region's nucleotide sequence