Experiment: WM_K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfaQ and rfaG overlap by 4 nucleotides rfaG and rfaP overlap by 8 nucleotides rfaP and waaO are separated by 42 nucleotides
JDDGAC_19275: rfaQ - lipopolysaccharide core heptosyltransferase RfaQ, at 3,666,023 to 3,667,081
rfaQ
JDDGAC_19280: rfaG - Lipopolysaccharide core biosynthesis protein RfaG, at 3,667,078 to 3,668,202
rfaG
JDDGAC_19285: rfaP - lipopolysaccharide core heptose(I) kinase RfaP, at 3,668,195 to 3,668,992
rfaP
JDDGAC_19290: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 3,669,035 to 3,670,042
waaO
Position (kb)
3667
3668
3669 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 3666.088 kb on + strand at 3666.100 kb on + strand at 3666.100 kb on + strand at 3666.268 kb on - strand, within rfaQ at 3666.268 kb on - strand, within rfaQ at 3666.270 kb on + strand, within rfaQ at 3666.270 kb on + strand, within rfaQ at 3666.271 kb on - strand, within rfaQ at 3666.271 kb on - strand, within rfaQ at 3666.271 kb on - strand, within rfaQ at 3666.280 kb on + strand, within rfaQ at 3666.280 kb on + strand, within rfaQ at 3666.284 kb on + strand, within rfaQ at 3666.325 kb on + strand, within rfaQ at 3666.325 kb on + strand, within rfaQ at 3666.326 kb on - strand, within rfaQ at 3666.326 kb on - strand, within rfaQ at 3666.328 kb on - strand, within rfaQ at 3666.451 kb on + strand, within rfaQ at 3666.535 kb on + strand, within rfaQ at 3666.575 kb on + strand, within rfaQ at 3666.575 kb on + strand, within rfaQ at 3666.592 kb on + strand, within rfaQ at 3666.593 kb on - strand, within rfaQ at 3666.654 kb on + strand, within rfaQ at 3666.655 kb on - strand, within rfaQ at 3666.655 kb on - strand, within rfaQ at 3666.697 kb on + strand, within rfaQ at 3666.866 kb on - strand, within rfaQ at 3666.998 kb on + strand at 3666.999 kb on - strand at 3667.042 kb on + strand at 3667.042 kb on + strand at 3667.069 kb on + strand at 3667.078 kb on - strand at 3667.098 kb on - strand at 3667.102 kb on - strand at 3667.220 kb on + strand, within rfaG at 3667.280 kb on - strand, within rfaG at 3667.512 kb on + strand, within rfaG at 3667.566 kb on + strand, within rfaG at 3667.571 kb on + strand, within rfaG at 3667.572 kb on - strand, within rfaG at 3667.666 kb on + strand, within rfaG at 3667.671 kb on - strand, within rfaG at 3667.701 kb on + strand, within rfaG at 3667.769 kb on + strand, within rfaG at 3667.769 kb on + strand, within rfaG at 3667.769 kb on + strand, within rfaG at 3667.770 kb on - strand, within rfaG at 3667.771 kb on + strand, within rfaG at 3667.950 kb on + strand, within rfaG at 3667.967 kb on + strand, within rfaG at 3667.999 kb on + strand, within rfaG at 3668.000 kb on - strand, within rfaG at 3668.000 kb on - strand, within rfaG at 3668.002 kb on - strand, within rfaG at 3668.002 kb on - strand, within rfaG at 3668.128 kb on + strand at 3668.136 kb on + strand at 3668.136 kb on + strand at 3668.180 kb on + strand at 3668.333 kb on + strand, within rfaP at 3668.406 kb on + strand, within rfaP at 3668.467 kb on + strand, within rfaP at 3668.467 kb on + strand, within rfaP at 3668.467 kb on + strand, within rfaP at 3668.467 kb on + strand, within rfaP at 3668.467 kb on + strand, within rfaP at 3668.554 kb on - strand, within rfaP at 3668.614 kb on + strand, within rfaP at 3668.685 kb on - strand, within rfaP at 3668.686 kb on + strand, within rfaP at 3668.873 kb on - strand, within rfaP at 3669.003 kb on - strand at 3669.120 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_K30 remove 3,666,088 + +0.6 3,666,100 + -0.2 3,666,100 + -0.8 3,666,268 - rfaQ JDDGAC_19275 0.23 -4.3 3,666,268 - rfaQ JDDGAC_19275 0.23 -1.8 3,666,270 + rfaQ JDDGAC_19275 0.23 -0.3 3,666,270 + rfaQ JDDGAC_19275 0.23 -0.1 3,666,271 - rfaQ JDDGAC_19275 0.23 -0.8 3,666,271 - rfaQ JDDGAC_19275 0.23 -2.3 3,666,271 - rfaQ JDDGAC_19275 0.23 -2.4 3,666,280 + rfaQ JDDGAC_19275 0.24 -0.0 3,666,280 + rfaQ JDDGAC_19275 0.24 -0.2 3,666,284 + rfaQ JDDGAC_19275 0.25 -0.1 3,666,325 + rfaQ JDDGAC_19275 0.29 -0.2 3,666,325 + rfaQ JDDGAC_19275 0.29 -2.1 3,666,326 - rfaQ JDDGAC_19275 0.29 -0.0 3,666,326 - rfaQ JDDGAC_19275 0.29 +0.0 3,666,328 - rfaQ JDDGAC_19275 0.29 -3.6 3,666,451 + rfaQ JDDGAC_19275 0.40 -0.1 3,666,535 + rfaQ JDDGAC_19275 0.48 -0.5 3,666,575 + rfaQ JDDGAC_19275 0.52 -1.5 3,666,575 + rfaQ JDDGAC_19275 0.52 -0.4 3,666,592 + rfaQ JDDGAC_19275 0.54 -0.2 3,666,593 - rfaQ JDDGAC_19275 0.54 +0.2 3,666,654 + rfaQ JDDGAC_19275 0.60 -0.7 3,666,655 - rfaQ JDDGAC_19275 0.60 -2.0 3,666,655 - rfaQ JDDGAC_19275 0.60 -4.1 3,666,697 + rfaQ JDDGAC_19275 0.64 -0.9 3,666,866 - rfaQ JDDGAC_19275 0.80 -0.4 3,666,998 + -1.5 3,666,999 - -2.4 3,667,042 + +1.3 3,667,042 + -0.6 3,667,069 + +0.2 3,667,078 - -3.4 3,667,098 - -2.4 3,667,102 - -2.5 3,667,220 + rfaG JDDGAC_19280 0.13 -2.0 3,667,280 - rfaG JDDGAC_19280 0.18 -5.2 3,667,512 + rfaG JDDGAC_19280 0.39 -2.5 3,667,566 + rfaG JDDGAC_19280 0.43 -3.1 3,667,571 + rfaG JDDGAC_19280 0.44 -3.4 3,667,572 - rfaG JDDGAC_19280 0.44 -7.0 3,667,666 + rfaG JDDGAC_19280 0.52 -0.9 3,667,671 - rfaG JDDGAC_19280 0.53 -3.9 3,667,701 + rfaG JDDGAC_19280 0.55 -0.8 3,667,769 + rfaG JDDGAC_19280 0.61 -2.5 3,667,769 + rfaG JDDGAC_19280 0.61 -2.0 3,667,769 + rfaG JDDGAC_19280 0.61 -2.9 3,667,770 - rfaG JDDGAC_19280 0.62 -0.6 3,667,771 + rfaG JDDGAC_19280 0.62 -1.9 3,667,950 + rfaG JDDGAC_19280 0.78 -2.3 3,667,967 + rfaG JDDGAC_19280 0.79 -0.8 3,667,999 + rfaG JDDGAC_19280 0.82 -2.2 3,668,000 - rfaG JDDGAC_19280 0.82 -2.2 3,668,000 - rfaG JDDGAC_19280 0.82 -2.6 3,668,002 - rfaG JDDGAC_19280 0.82 -2.4 3,668,002 - rfaG JDDGAC_19280 0.82 -3.6 3,668,128 + -4.2 3,668,136 + -1.2 3,668,136 + +0.8 3,668,180 + +0.0 3,668,333 + rfaP JDDGAC_19285 0.17 -2.4 3,668,406 + rfaP JDDGAC_19285 0.26 -0.2 3,668,467 + rfaP JDDGAC_19285 0.34 +0.3 3,668,467 + rfaP JDDGAC_19285 0.34 -1.0 3,668,467 + rfaP JDDGAC_19285 0.34 -0.1 3,668,467 + rfaP JDDGAC_19285 0.34 -1.9 3,668,467 + rfaP JDDGAC_19285 0.34 -1.1 3,668,554 - rfaP JDDGAC_19285 0.45 -1.1 3,668,614 + rfaP JDDGAC_19285 0.53 -0.6 3,668,685 - rfaP JDDGAC_19285 0.61 -2.9 3,668,686 + rfaP JDDGAC_19285 0.62 -0.4 3,668,873 - rfaP JDDGAC_19285 0.85 -2.2 3,669,003 - -2.9 3,669,120 + -2.8
Or see this region's nucleotide sequence