Experiment: WM_K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaaA and yaaX are separated by 152 nucleotides yaaX and thrC are separated by 213 nucleotides thrC and thrB are separated by 0 nucleotides thrB and thrA are separated by 1 nucleotides
JDDGAC_14685: yaaA - peroxide stress protein YaaA, at 2,739,509 to 2,740,285
yaaA
JDDGAC_14690: yaaX - Uncharacterized protein YaaX, at 2,740,438 to 2,740,734
yaaX
JDDGAC_14695: thrC - threonine synthase, at 2,740,948 to 2,742,234
thrC
JDDGAC_14700: thrB - homoserine kinase, at 2,742,235 to 2,743,167
thrB
JDDGAC_14705: thrA - bifunctional aspartate kinase/homoserine dehydrogenase I, at 2,743,169 to 2,745,631
thrA
Position (kb)
2740
2741
2742
2743 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2739.955 kb on + strand, within yaaA at 2739.955 kb on + strand, within yaaA at 2739.956 kb on - strand, within yaaA at 2739.956 kb on - strand, within yaaA at 2740.031 kb on + strand, within yaaA at 2740.037 kb on - strand, within yaaA at 2740.116 kb on + strand, within yaaA at 2740.127 kb on + strand, within yaaA at 2740.127 kb on + strand, within yaaA at 2740.127 kb on + strand, within yaaA at 2740.127 kb on + strand, within yaaA at 2740.127 kb on + strand, within yaaA at 2740.128 kb on - strand, within yaaA at 2740.128 kb on - strand, within yaaA at 2740.132 kb on + strand, within yaaA at 2740.207 kb on + strand, within yaaA at 2740.208 kb on - strand at 2740.221 kb on - strand at 2740.283 kb on + strand at 2740.494 kb on + strand, within yaaX at 2740.494 kb on + strand, within yaaX at 2740.554 kb on - strand, within yaaX at 2740.554 kb on - strand, within yaaX at 2740.628 kb on - strand, within yaaX at 2740.634 kb on - strand, within yaaX at 2740.656 kb on + strand, within yaaX at 2740.716 kb on + strand at 2740.717 kb on - strand at 2740.740 kb on - strand at 2740.748 kb on + strand at 2740.749 kb on - strand at 2740.749 kb on - strand at 2740.880 kb on + strand at 2740.935 kb on + strand at 2740.936 kb on - strand at 2740.936 kb on - strand at 2740.942 kb on + strand at 2740.943 kb on - strand at 2740.943 kb on - strand at 2740.949 kb on + strand at 2740.950 kb on - strand at 2740.950 kb on - strand at 2740.971 kb on + strand at 2740.972 kb on - strand at 2741.018 kb on - strand at 2741.192 kb on + strand, within thrC at 2741.206 kb on + strand, within thrC at 2741.206 kb on + strand, within thrC at 2741.252 kb on + strand, within thrC at 2741.252 kb on + strand, within thrC at 2741.427 kb on + strand, within thrC at 2741.559 kb on - strand, within thrC at 2741.862 kb on + strand, within thrC at 2741.916 kb on + strand, within thrC at 2741.917 kb on - strand, within thrC at 2742.217 kb on - strand at 2742.225 kb on - strand at 2742.279 kb on + strand at 2742.279 kb on + strand at 2742.280 kb on - strand at 2742.308 kb on + strand at 2742.309 kb on - strand at 2742.394 kb on - strand, within thrB at 2742.480 kb on + strand, within thrB at 2742.481 kb on - strand, within thrB at 2742.627 kb on + strand, within thrB at 2742.749 kb on + strand, within thrB at 2742.749 kb on + strand, within thrB at 2742.750 kb on - strand, within thrB at 2742.881 kb on - strand, within thrB at 2742.900 kb on + strand, within thrB at 2742.972 kb on - strand, within thrB at 2742.978 kb on + strand, within thrB at 2743.063 kb on + strand, within thrB at 2743.063 kb on + strand, within thrB at 2743.063 kb on + strand, within thrB at 2743.178 kb on + strand at 2743.179 kb on - strand at 2743.179 kb on - strand at 2743.195 kb on + strand at 2743.199 kb on + strand at 2743.200 kb on - strand at 2743.200 kb on - strand at 2743.200 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_K30 remove 2,739,955 + yaaA JDDGAC_14685 0.57 -0.3 2,739,955 + yaaA JDDGAC_14685 0.57 -0.1 2,739,956 - yaaA JDDGAC_14685 0.58 -3.1 2,739,956 - yaaA JDDGAC_14685 0.58 -0.2 2,740,031 + yaaA JDDGAC_14685 0.67 -1.1 2,740,037 - yaaA JDDGAC_14685 0.68 +0.6 2,740,116 + yaaA JDDGAC_14685 0.78 +1.0 2,740,127 + yaaA JDDGAC_14685 0.80 +1.7 2,740,127 + yaaA JDDGAC_14685 0.80 +0.0 2,740,127 + yaaA JDDGAC_14685 0.80 +0.2 2,740,127 + yaaA JDDGAC_14685 0.80 +1.0 2,740,127 + yaaA JDDGAC_14685 0.80 -0.4 2,740,128 - yaaA JDDGAC_14685 0.80 +0.6 2,740,128 - yaaA JDDGAC_14685 0.80 +0.0 2,740,132 + yaaA JDDGAC_14685 0.80 -0.1 2,740,207 + yaaA JDDGAC_14685 0.90 -0.5 2,740,208 - +0.2 2,740,221 - +0.4 2,740,283 + -0.3 2,740,494 + yaaX JDDGAC_14690 0.19 +0.3 2,740,494 + yaaX JDDGAC_14690 0.19 +0.7 2,740,554 - yaaX JDDGAC_14690 0.39 +0.4 2,740,554 - yaaX JDDGAC_14690 0.39 +0.4 2,740,628 - yaaX JDDGAC_14690 0.64 +1.1 2,740,634 - yaaX JDDGAC_14690 0.66 -0.1 2,740,656 + yaaX JDDGAC_14690 0.73 -0.3 2,740,716 + -0.9 2,740,717 - +0.0 2,740,740 - +0.6 2,740,748 + -0.2 2,740,749 - -0.6 2,740,749 - -0.0 2,740,880 + -0.9 2,740,935 + -0.9 2,740,936 - +1.8 2,740,936 - +0.5 2,740,942 + +0.6 2,740,943 - -0.0 2,740,943 - -0.2 2,740,949 + -0.5 2,740,950 - +0.1 2,740,950 - -1.2 2,740,971 + +0.2 2,740,972 - +1.0 2,741,018 - -1.5 2,741,192 + thrC JDDGAC_14695 0.19 -0.5 2,741,206 + thrC JDDGAC_14695 0.20 -0.7 2,741,206 + thrC JDDGAC_14695 0.20 -1.6 2,741,252 + thrC JDDGAC_14695 0.24 -2.1 2,741,252 + thrC JDDGAC_14695 0.24 -1.9 2,741,427 + thrC JDDGAC_14695 0.37 -2.0 2,741,559 - thrC JDDGAC_14695 0.47 -0.9 2,741,862 + thrC JDDGAC_14695 0.71 -2.2 2,741,916 + thrC JDDGAC_14695 0.75 -0.8 2,741,917 - thrC JDDGAC_14695 0.75 +0.3 2,742,217 - -1.8 2,742,225 - -0.8 2,742,279 + -0.9 2,742,279 + -1.5 2,742,280 - -1.0 2,742,308 + -1.4 2,742,309 - -1.2 2,742,394 - thrB JDDGAC_14700 0.17 -0.6 2,742,480 + thrB JDDGAC_14700 0.26 -0.7 2,742,481 - thrB JDDGAC_14700 0.26 -1.4 2,742,627 + thrB JDDGAC_14700 0.42 -1.3 2,742,749 + thrB JDDGAC_14700 0.55 -0.9 2,742,749 + thrB JDDGAC_14700 0.55 -1.1 2,742,750 - thrB JDDGAC_14700 0.55 -1.7 2,742,881 - thrB JDDGAC_14700 0.69 -1.2 2,742,900 + thrB JDDGAC_14700 0.71 -2.9 2,742,972 - thrB JDDGAC_14700 0.79 -2.0 2,742,978 + thrB JDDGAC_14700 0.80 -1.0 2,743,063 + thrB JDDGAC_14700 0.89 -1.1 2,743,063 + thrB JDDGAC_14700 0.89 -1.9 2,743,063 + thrB JDDGAC_14700 0.89 -1.7 2,743,178 + -1.2 2,743,179 - -2.6 2,743,179 - -0.9 2,743,195 + -0.1 2,743,199 + -1.9 2,743,200 - -0.4 2,743,200 - -0.2 2,743,200 - -0.2
Or see this region's nucleotide sequence