Experiment: WM_K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tsx and yajD are separated by 176 nucleotides yajD and JDDGAC_12135 are separated by 87 nucleotides JDDGAC_12135 and parD overlap by 8 nucleotides parD and secF are separated by 151 nucleotides
JDDGAC_12125: tsx - nucleoside-specific channel-forming protein Tsx, at 2,228,670 to 2,229,554
tsx
JDDGAC_12130: yajD - HNH nuclease YajD, at 2,229,731 to 2,230,078
yajD
JDDGAC_12135: JDDGAC_12135 - type II toxin-antitoxin system RelE/ParE family toxin, at 2,230,166 to 2,230,447
_12135
JDDGAC_12140: parD - Antitoxin ParD, at 2,230,440 to 2,230,682
parD
JDDGAC_12145: secF - Protein translocase subunit SecF, at 2,230,834 to 2,231,805
secF
Position (kb)
2230
2231 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2229.538 kb on + strand at 2229.538 kb on + strand at 2229.539 kb on - strand at 2229.543 kb on + strand at 2229.543 kb on + strand at 2229.543 kb on + strand at 2229.544 kb on - strand at 2229.597 kb on - strand at 2229.605 kb on - strand at 2229.777 kb on + strand, within yajD at 2229.777 kb on + strand, within yajD at 2229.778 kb on - strand, within yajD at 2229.778 kb on - strand, within yajD at 2229.830 kb on - strand, within yajD at 2229.902 kb on - strand, within yajD at 2229.925 kb on + strand, within yajD at 2229.925 kb on + strand, within yajD at 2229.952 kb on + strand, within yajD at 2230.056 kb on + strand at 2230.073 kb on - strand at 2230.073 kb on - strand at 2230.101 kb on + strand at 2230.233 kb on - strand, within JDDGAC_12135 at 2230.233 kb on - strand, within JDDGAC_12135 at 2230.239 kb on + strand, within JDDGAC_12135 at 2230.239 kb on + strand, within JDDGAC_12135 at 2230.243 kb on - strand, within JDDGAC_12135 at 2230.274 kb on + strand, within JDDGAC_12135 at 2230.275 kb on - strand, within JDDGAC_12135 at 2230.293 kb on + strand, within JDDGAC_12135 at 2230.293 kb on + strand, within JDDGAC_12135 at 2230.293 kb on + strand, within JDDGAC_12135 at 2230.356 kb on + strand, within JDDGAC_12135 at 2230.396 kb on - strand, within JDDGAC_12135 at 2230.402 kb on - strand, within JDDGAC_12135 at 2230.402 kb on - strand, within JDDGAC_12135 at 2230.402 kb on - strand, within JDDGAC_12135 at 2230.441 kb on + strand at 2230.444 kb on - strand at 2230.444 kb on - strand at 2230.444 kb on - strand at 2230.444 kb on - strand at 2230.587 kb on + strand, within parD at 2230.640 kb on + strand, within parD at 2230.644 kb on + strand, within parD at 2230.644 kb on + strand, within parD at 2230.662 kb on + strand at 2230.707 kb on + strand at 2230.708 kb on - strand at 2230.717 kb on + strand at 2230.718 kb on - strand at 2230.718 kb on - strand at 2230.718 kb on - strand at 2230.719 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_K30 remove 2,229,538 + +0.9 2,229,538 + -1.2 2,229,539 - -1.3 2,229,543 + +0.4 2,229,543 + +0.4 2,229,543 + +0.2 2,229,544 - +0.2 2,229,597 - +0.2 2,229,605 - +0.3 2,229,777 + yajD JDDGAC_12130 0.13 -1.2 2,229,777 + yajD JDDGAC_12130 0.13 +0.5 2,229,778 - yajD JDDGAC_12130 0.14 +0.6 2,229,778 - yajD JDDGAC_12130 0.14 -2.3 2,229,830 - yajD JDDGAC_12130 0.28 -0.5 2,229,902 - yajD JDDGAC_12130 0.49 -0.3 2,229,925 + yajD JDDGAC_12130 0.56 +0.8 2,229,925 + yajD JDDGAC_12130 0.56 -0.8 2,229,952 + yajD JDDGAC_12130 0.64 -0.2 2,230,056 + +0.7 2,230,073 - -0.6 2,230,073 - -0.3 2,230,101 + -3.1 2,230,233 - JDDGAC_12135 0.24 +0.4 2,230,233 - JDDGAC_12135 0.24 -3.2 2,230,239 + JDDGAC_12135 0.26 -0.4 2,230,239 + JDDGAC_12135 0.26 +0.3 2,230,243 - JDDGAC_12135 0.27 -0.3 2,230,274 + JDDGAC_12135 0.38 +1.4 2,230,275 - JDDGAC_12135 0.39 +0.5 2,230,293 + JDDGAC_12135 0.45 -0.8 2,230,293 + JDDGAC_12135 0.45 -0.3 2,230,293 + JDDGAC_12135 0.45 +0.7 2,230,356 + JDDGAC_12135 0.67 +0.4 2,230,396 - JDDGAC_12135 0.82 +0.1 2,230,402 - JDDGAC_12135 0.84 +0.1 2,230,402 - JDDGAC_12135 0.84 -0.3 2,230,402 - JDDGAC_12135 0.84 +1.1 2,230,441 + -0.1 2,230,444 - -1.2 2,230,444 - +0.3 2,230,444 - +0.3 2,230,444 - +0.3 2,230,587 + parD JDDGAC_12140 0.60 -3.1 2,230,640 + parD JDDGAC_12140 0.82 -1.9 2,230,644 + parD JDDGAC_12140 0.84 +0.9 2,230,644 + parD JDDGAC_12140 0.84 -0.4 2,230,662 + +0.1 2,230,707 + +0.4 2,230,708 - -0.4 2,230,717 + -0.3 2,230,718 - -1.4 2,230,718 - -0.2 2,230,718 - -0.6 2,230,719 + +0.6
Or see this region's nucleotide sequence