Experiment: WM_K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt JDDGAC_07070 and JDDGAC_07075 are separated by 177 nucleotides JDDGAC_07075 and JDDGAC_07080 are separated by 61 nucleotides JDDGAC_07080 and JDDGAC_07085 overlap by 32 nucleotides JDDGAC_07085 and potC are separated by 166 nucleotides potC and potD overlap by 4 nucleotides
JDDGAC_07070: JDDGAC_07070 - hypothetical protein, at 1,220,834 to 1,221,349
_07070
JDDGAC_07075: JDDGAC_07075 - 3'-5' exoribonuclease, at 1,221,527 to 1,221,871
_07075
JDDGAC_07080: JDDGAC_07080 - excisionase, at 1,221,933 to 1,222,202
_07080
JDDGAC_07085: JDDGAC_07085 - integrase, at 1,222,171 to 1,223,289
_07085
JDDGAC_07090: potC - spermidine/putrescine ABC transporter permease PotC, at 1,223,456 to 1,224,250
potC
JDDGAC_07095: potD - spermidine/putrescine ABC transporter substrate-binding protein PotD, at 1,224,247 to 1,225,293
potD
Position (kb)
1222
1223
1224 Strain fitness (log2 ratio)
-2
-1
0
1 at 1221.174 kb on + strand, within JDDGAC_07070 at 1221.217 kb on + strand, within JDDGAC_07070 at 1221.335 kb on + strand at 1221.485 kb on - strand at 1221.485 kb on - strand at 1221.497 kb on - strand at 1221.900 kb on - strand at 1222.004 kb on + strand, within JDDGAC_07080 at 1222.004 kb on + strand, within JDDGAC_07080 at 1222.027 kb on + strand, within JDDGAC_07080 at 1222.032 kb on + strand, within JDDGAC_07080 at 1222.032 kb on + strand, within JDDGAC_07080 at 1222.033 kb on - strand, within JDDGAC_07080 at 1222.166 kb on - strand, within JDDGAC_07080 at 1222.216 kb on + strand at 1222.346 kb on + strand, within JDDGAC_07085 at 1222.482 kb on + strand, within JDDGAC_07085 at 1222.525 kb on + strand, within JDDGAC_07085 at 1222.525 kb on + strand, within JDDGAC_07085 at 1222.572 kb on - strand, within JDDGAC_07085 at 1222.739 kb on + strand, within JDDGAC_07085 at 1222.945 kb on - strand, within JDDGAC_07085 at 1222.949 kb on + strand, within JDDGAC_07085 at 1223.009 kb on + strand, within JDDGAC_07085 at 1223.012 kb on + strand, within JDDGAC_07085 at 1223.012 kb on + strand, within JDDGAC_07085 at 1223.020 kb on + strand, within JDDGAC_07085 at 1223.024 kb on + strand, within JDDGAC_07085 at 1223.031 kb on + strand, within JDDGAC_07085 at 1223.210 kb on + strand at 1223.211 kb on - strand at 1223.220 kb on + strand at 1223.284 kb on - strand at 1223.284 kb on - strand at 1223.306 kb on + strand at 1223.510 kb on + strand at 1223.594 kb on + strand, within potC at 1223.596 kb on + strand, within potC at 1223.597 kb on - strand, within potC at 1223.625 kb on + strand, within potC at 1223.812 kb on + strand, within potC at 1224.162 kb on + strand, within potC at 1224.162 kb on + strand, within potC at 1224.206 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_K30 remove 1,221,174 + JDDGAC_07070 0.66 -2.5 1,221,217 + JDDGAC_07070 0.74 +0.0 1,221,335 + -0.6 1,221,485 - +1.4 1,221,485 - +0.1 1,221,497 - -0.5 1,221,900 - -0.1 1,222,004 + JDDGAC_07080 0.26 +0.9 1,222,004 + JDDGAC_07080 0.26 -0.3 1,222,027 + JDDGAC_07080 0.35 -1.8 1,222,032 + JDDGAC_07080 0.37 +0.6 1,222,032 + JDDGAC_07080 0.37 -0.9 1,222,033 - JDDGAC_07080 0.37 +0.3 1,222,166 - JDDGAC_07080 0.86 +0.4 1,222,216 + -0.7 1,222,346 + JDDGAC_07085 0.16 -1.9 1,222,482 + JDDGAC_07085 0.28 -0.4 1,222,525 + JDDGAC_07085 0.32 -0.4 1,222,525 + JDDGAC_07085 0.32 -0.0 1,222,572 - JDDGAC_07085 0.36 -0.2 1,222,739 + JDDGAC_07085 0.51 -0.3 1,222,945 - JDDGAC_07085 0.69 +0.2 1,222,949 + JDDGAC_07085 0.70 +0.7 1,223,009 + JDDGAC_07085 0.75 +0.2 1,223,012 + JDDGAC_07085 0.75 +0.2 1,223,012 + JDDGAC_07085 0.75 -0.9 1,223,020 + JDDGAC_07085 0.76 -0.1 1,223,024 + JDDGAC_07085 0.76 -0.3 1,223,031 + JDDGAC_07085 0.77 +1.4 1,223,210 + -0.3 1,223,211 - +1.7 1,223,220 + -0.3 1,223,284 - -0.8 1,223,284 - -0.0 1,223,306 + +0.1 1,223,510 + +0.7 1,223,594 + potC JDDGAC_07090 0.17 -0.4 1,223,596 + potC JDDGAC_07090 0.18 +0.2 1,223,597 - potC JDDGAC_07090 0.18 -0.6 1,223,625 + potC JDDGAC_07090 0.21 -0.2 1,223,812 + potC JDDGAC_07090 0.45 +0.3 1,224,162 + potC JDDGAC_07090 0.89 +0.6 1,224,162 + potC JDDGAC_07090 0.89 -1.2 1,224,206 - -0.0
Or see this region's nucleotide sequence