Strain Fitness in Escherichia coli ECRC98 around JDDGAC_06625

Experiment: WM_K30

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmnmA and hflD are separated by 35 nucleotideshflD and purB are separated by 3 nucleotides JDDGAC_06620: mnmA - tRNA 2-thiouridine(34) synthase MnmA, at 1,153,666 to 1,154,772 mnmA JDDGAC_06625: hflD - high frequency lysogenization protein HflD, at 1,154,808 to 1,155,449 hflD JDDGAC_06630: purB - adenylosuccinate lyase, at 1,155,453 to 1,156,823 purB Position (kb) 1154 1155 1156Strain fitness (log2 ratio) -2 -1 0 1at 1154.032 kb on + strand, within mnmAat 1154.123 kb on - strand, within mnmAat 1154.575 kb on - strand, within mnmAat 1154.710 kb on + strandat 1154.774 kb on + strandat 1154.777 kb on + strandat 1154.787 kb on + strandat 1154.933 kb on + strand, within hflDat 1154.934 kb on - strand, within hflDat 1154.942 kb on + strand, within hflDat 1155.049 kb on + strand, within hflDat 1155.215 kb on + strand, within hflDat 1155.219 kb on + strand, within hflDat 1155.219 kb on + strand, within hflDat 1155.219 kb on + strand, within hflDat 1155.393 kb on - strandat 1155.433 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_K30
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1,154,032 + mnmA JDDGAC_06620 0.33 -1.2
1,154,123 - mnmA JDDGAC_06620 0.41 -1.4
1,154,575 - mnmA JDDGAC_06620 0.82 +0.6
1,154,710 + +0.5
1,154,774 + -0.1
1,154,777 + -0.9
1,154,787 + +0.5
1,154,933 + hflD JDDGAC_06625 0.19 -0.4
1,154,934 - hflD JDDGAC_06625 0.20 -0.8
1,154,942 + hflD JDDGAC_06625 0.21 -1.8
1,155,049 + hflD JDDGAC_06625 0.38 -1.4
1,155,215 + hflD JDDGAC_06625 0.63 -1.5
1,155,219 + hflD JDDGAC_06625 0.64 -0.2
1,155,219 + hflD JDDGAC_06625 0.64 +0.6
1,155,219 + hflD JDDGAC_06625 0.64 -1.0
1,155,393 - -0.4
1,155,433 + -1.3

Or see this region's nucleotide sequence