Experiment: WM_K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt acnA and ymiC are separated by 163 nucleotides ymiC and JDDGAC_05135 are separated by 128 nucleotides JDDGAC_05135 and ymiA are separated by 2 nucleotides ymiA and cysB are separated by 330 nucleotides
JDDGAC_05120: acnA - aconitate hydratase AcnA, at 898,696 to 901,371
acnA
JDDGAC_05130: ymiC - Protein YmiC, at 901,535 to 901,615
ymiC
JDDGAC_05135: JDDGAC_05135 - Protein, C-ter, truncated protein, at 901,744 to 901,911
_05135
JDDGAC_05140: ymiA - YmiA family putative membrane protein, at 901,914 to 902,042
ymiA
JDDGAC_05145: cysB - HTH-type transcriptional regulator CysB, at 902,373 to 903,347
cysB
Position (kb)
901
902 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 900.902 kb on + strand, within acnA at 900.903 kb on - strand, within acnA at 900.903 kb on - strand, within acnA at 900.935 kb on - strand, within acnA at 901.206 kb on + strand at 901.304 kb on + strand at 901.304 kb on + strand at 901.305 kb on - strand at 901.344 kb on + strand at 901.631 kb on - strand at 901.631 kb on - strand at 901.636 kb on - strand at 901.654 kb on - strand at 901.654 kb on - strand at 901.681 kb on + strand at 901.746 kb on - strand at 901.746 kb on - strand at 901.805 kb on - strand, within JDDGAC_05135 at 901.805 kb on - strand, within JDDGAC_05135 at 901.867 kb on - strand, within JDDGAC_05135 at 901.912 kb on - strand at 901.912 kb on - strand at 901.912 kb on - strand at 901.912 kb on - strand at 901.912 kb on - strand at 901.912 kb on - strand at 901.916 kb on - strand at 902.057 kb on - strand at 902.237 kb on - strand at 902.248 kb on + strand at 902.267 kb on - strand at 902.324 kb on + strand at 902.391 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_K30 remove 900,902 + acnA JDDGAC_05120 0.82 +0.5 900,903 - acnA JDDGAC_05120 0.82 +1.2 900,903 - acnA JDDGAC_05120 0.82 -0.9 900,935 - acnA JDDGAC_05120 0.84 +0.0 901,206 + -0.3 901,304 + +1.0 901,304 + +0.6 901,305 - +0.8 901,344 + -1.3 901,631 - -0.5 901,631 - +0.0 901,636 - -0.2 901,654 - +0.4 901,654 - +0.6 901,681 + -1.9 901,746 - +0.0 901,746 - -3.0 901,805 - JDDGAC_05135 0.36 -0.3 901,805 - JDDGAC_05135 0.36 -0.4 901,867 - JDDGAC_05135 0.73 -2.0 901,912 - +0.7 901,912 - -0.0 901,912 - -1.3 901,912 - +1.0 901,912 - +0.4 901,912 - +0.2 901,916 - +1.4 902,057 - -0.7 902,237 - -0.3 902,248 + +0.7 902,267 - +0.8 902,324 + -0.4 902,391 + -0.6
Or see this region's nucleotide sequence