Strain Fitness in Escherichia coli ECRC98 around JDDGAC_03625

Experiment: WM_K30

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsad and glsB are separated by 63 nucleotidesglsB and yneG overlap by 1 nucleotidesyneG and gGDEF are separated by 138 nucleotides JDDGAC_03620: sad - succinate-semialdehyde dehydrogenase, at 601,411 to 602,799 sad JDDGAC_03625: glsB - glutaminase B, at 602,863 to 603,789 glsB JDDGAC_03630: yneG - Uncharacterized protein YneG, at 603,789 to 604,148 yneG JDDGAC_03635: gGDEF - diguanylate cyclase, at 604,287 to 605,705 gGDEF Position (kb) 602 603 604Strain fitness (log2 ratio) -2 -1 0 1 2at 601.883 kb on + strand, within sadat 601.884 kb on - strand, within sadat 601.964 kb on + strand, within sadat 601.964 kb on + strand, within sadat 602.133 kb on + strand, within sadat 602.169 kb on + strand, within sadat 602.443 kb on - strand, within sadat 602.753 kb on + strandat 602.753 kb on + strandat 603.210 kb on + strand, within glsBat 603.210 kb on + strand, within glsBat 603.237 kb on + strand, within glsBat 603.273 kb on + strand, within glsBat 603.273 kb on + strand, within glsBat 603.273 kb on + strand, within glsBat 603.516 kb on + strand, within glsBat 603.588 kb on + strand, within glsBat 603.787 kb on + strandat 604.043 kb on + strand, within yneGat 604.170 kb on + strandat 604.190 kb on + strandat 604.191 kb on - strandat 604.219 kb on - strandat 604.243 kb on - strandat 604.271 kb on + strandat 604.286 kb on - strandat 604.295 kb on - strandat 604.346 kb on + strandat 604.399 kb on + strandat 604.479 kb on - strand, within gGDEFat 604.580 kb on + strand, within gGDEFat 604.663 kb on + strand, within gGDEFat 604.673 kb on + strand, within gGDEF

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_K30
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601,883 + sad JDDGAC_03620 0.34 -0.6
601,884 - sad JDDGAC_03620 0.34 +2.2
601,964 + sad JDDGAC_03620 0.40 +0.2
601,964 + sad JDDGAC_03620 0.40 -0.6
602,133 + sad JDDGAC_03620 0.52 -0.6
602,169 + sad JDDGAC_03620 0.55 -1.2
602,443 - sad JDDGAC_03620 0.74 +0.2
602,753 + -0.9
602,753 + +0.6
603,210 + glsB JDDGAC_03625 0.37 -0.2
603,210 + glsB JDDGAC_03625 0.37 +1.0
603,237 + glsB JDDGAC_03625 0.40 -0.5
603,273 + glsB JDDGAC_03625 0.44 +0.6
603,273 + glsB JDDGAC_03625 0.44 -2.2
603,273 + glsB JDDGAC_03625 0.44 +0.5
603,516 + glsB JDDGAC_03625 0.70 +0.2
603,588 + glsB JDDGAC_03625 0.78 +2.1
603,787 + -0.3
604,043 + yneG JDDGAC_03630 0.71 +0.3
604,170 + -0.8
604,190 + -1.3
604,191 - +0.1
604,219 - -0.4
604,243 - -0.6
604,271 + -0.1
604,286 - +0.2
604,295 - +0.4
604,346 + +0.3
604,399 + +0.2
604,479 - gGDEF JDDGAC_03635 0.14 -2.7
604,580 + gGDEF JDDGAC_03635 0.21 -0.9
604,663 + gGDEF JDDGAC_03635 0.26 -0.2
604,673 + gGDEF JDDGAC_03635 0.27 -2.3

Or see this region's nucleotide sequence