Experiment: WM_K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nimT and nimR are separated by 99 nucleotides nimR and yeaL overlap by 44 nucleotides yeaL and yeaK are separated by 274 nucleotides yeaK and dgcJ are separated by 42 nucleotides
JDDGAC_01250: nimT - 2-nitroimidazole transporter, at 210,780 to 211,961
nimT
JDDGAC_01255: nimR - DNA-binding transcriptional regulator NimR, at 212,061 to 212,879
nimR
JDDGAC_01260: yeaL - UPF0756 membrane protein CKO_01811, at 212,836 to 213,282
yeaL
JDDGAC_01265: yeaK - mischarged aminoacyl-tRNA deacylase, at 213,557 to 214,060
yeaK
JDDGAC_01270: dgcJ - diguanylate cyclase DgcJ, at 214,103 to 215,593
dgcJ
Position (kb) 212
213
214 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 211.962 kb on - strand at 211.962 kb on - strand at 211.962 kb on - strand at 211.962 kb on - strand at 212.161 kb on + strand, within nimR at 212.161 kb on + strand, within nimR at 212.198 kb on - strand, within nimR at 212.253 kb on + strand, within nimR at 212.285 kb on + strand, within nimR at 212.285 kb on + strand, within nimR at 212.286 kb on - strand, within nimR at 212.365 kb on - strand, within nimR at 212.398 kb on + strand, within nimR at 212.687 kb on - strand, within nimR at 212.727 kb on - strand, within nimR at 212.731 kb on + strand, within nimR at 212.731 kb on + strand, within nimR at 212.777 kb on + strand, within nimR at 212.778 kb on - strand, within nimR at 212.784 kb on - strand, within nimR at 212.784 kb on - strand, within nimR at 212.838 kb on - strand at 212.878 kb on - strand at 212.917 kb on + strand, within yeaL at 213.011 kb on - strand, within yeaL at 213.222 kb on + strand, within yeaL at 213.222 kb on + strand, within yeaL at 213.317 kb on + strand at 213.322 kb on + strand at 213.323 kb on - strand at 213.433 kb on + strand at 213.433 kb on + strand at 213.554 kb on + strand at 213.555 kb on - strand at 213.805 kb on + strand, within yeaK at 213.806 kb on - strand, within yeaK at 213.892 kb on + strand, within yeaK at 213.897 kb on - strand, within yeaK at 213.897 kb on - strand, within yeaK at 213.908 kb on - strand, within yeaK at 213.908 kb on - strand, within yeaK at 213.921 kb on - strand, within yeaK at 213.958 kb on + strand, within yeaK at 213.963 kb on - strand, within yeaK at 214.138 kb on - strand at 214.140 kb on - strand at 214.173 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_K30 remove 211,962 - +0.1 211,962 - -1.2 211,962 - +0.3 211,962 - +0.9 212,161 + nimR JDDGAC_01255 0.12 +0.9 212,161 + nimR JDDGAC_01255 0.12 +0.6 212,198 - nimR JDDGAC_01255 0.17 +1.6 212,253 + nimR JDDGAC_01255 0.23 +1.0 212,285 + nimR JDDGAC_01255 0.27 +0.3 212,285 + nimR JDDGAC_01255 0.27 -0.6 212,286 - nimR JDDGAC_01255 0.27 +1.4 212,365 - nimR JDDGAC_01255 0.37 +1.2 212,398 + nimR JDDGAC_01255 0.41 -1.6 212,687 - nimR JDDGAC_01255 0.76 +0.6 212,727 - nimR JDDGAC_01255 0.81 -1.1 212,731 + nimR JDDGAC_01255 0.82 +0.9 212,731 + nimR JDDGAC_01255 0.82 -0.4 212,777 + nimR JDDGAC_01255 0.87 -0.2 212,778 - nimR JDDGAC_01255 0.88 +0.3 212,784 - nimR JDDGAC_01255 0.88 -0.4 212,784 - nimR JDDGAC_01255 0.88 -0.7 212,838 - +0.9 212,878 - -0.0 212,917 + yeaL JDDGAC_01260 0.18 -2.1 213,011 - yeaL JDDGAC_01260 0.39 -1.1 213,222 + yeaL JDDGAC_01260 0.86 -0.6 213,222 + yeaL JDDGAC_01260 0.86 +0.1 213,317 + -0.3 213,322 + +0.5 213,323 - -1.0 213,433 + +0.1 213,433 + -0.7 213,554 + -2.9 213,555 - -0.2 213,805 + yeaK JDDGAC_01265 0.49 -0.1 213,806 - yeaK JDDGAC_01265 0.49 +0.9 213,892 + yeaK JDDGAC_01265 0.66 +1.2 213,897 - yeaK JDDGAC_01265 0.67 +0.7 213,897 - yeaK JDDGAC_01265 0.67 +0.1 213,908 - yeaK JDDGAC_01265 0.70 -0.4 213,908 - yeaK JDDGAC_01265 0.70 -0.6 213,921 - yeaK JDDGAC_01265 0.72 -0.3 213,958 + yeaK JDDGAC_01265 0.80 +0.6 213,963 - yeaK JDDGAC_01265 0.81 -0.5 214,138 - +0.7 214,140 - -1.1 214,173 - +0.0
Or see this region's nucleotide sequence