Experiment: WM_K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nfeF and Ile2_trna are separated by 53 nucleotides Ile2_trna and mug are separated by 124 nucleotides mug and rpoD are separated by 78 nucleotides
JDDGAC_22275: nfeF - NADPH-dependent ferric chelate reductase, at 4,261,949 to 4,262,713
nfeF
JDDGAC_22280: Ile2_trna - tRNA-Ile2(cat), at 4,262,767 to 4,262,842
_trna
JDDGAC_22285: mug - G/U mismatch-specific DNA glycosylase, at 4,262,967 to 4,263,473
mug
JDDGAC_22290: rpoD - RNA polymerase sigma factor RpoD, at 4,263,552 to 4,265,393
rpoD
Position (kb)
4262
4263 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4261.802 kb on + strand at 4261.802 kb on + strand at 4261.803 kb on - strand at 4261.803 kb on - strand at 4261.842 kb on + strand at 4261.849 kb on - strand at 4261.849 kb on - strand at 4261.883 kb on + strand at 4261.955 kb on - strand at 4262.016 kb on + strand at 4262.030 kb on - strand, within nfeF at 4262.084 kb on - strand, within nfeF at 4262.210 kb on + strand, within nfeF at 4262.212 kb on + strand, within nfeF at 4262.213 kb on - strand, within nfeF at 4262.213 kb on - strand, within nfeF at 4262.232 kb on + strand, within nfeF at 4262.233 kb on - strand, within nfeF at 4262.233 kb on - strand, within nfeF at 4262.364 kb on - strand, within nfeF at 4262.378 kb on + strand, within nfeF at 4262.378 kb on + strand, within nfeF at 4262.378 kb on + strand, within nfeF at 4262.379 kb on - strand, within nfeF at 4262.379 kb on - strand, within nfeF at 4262.379 kb on - strand, within nfeF at 4262.458 kb on + strand, within nfeF at 4262.522 kb on - strand, within nfeF at 4262.556 kb on + strand, within nfeF at 4262.597 kb on + strand, within nfeF at 4262.598 kb on - strand, within nfeF at 4262.598 kb on - strand, within nfeF at 4262.667 kb on - strand at 4262.719 kb on - strand at 4262.720 kb on + strand at 4262.761 kb on + strand at 4262.761 kb on + strand at 4262.762 kb on - strand at 4262.762 kb on - strand at 4262.762 kb on - strand at 4262.764 kb on - strand at 4262.855 kb on + strand at 4262.858 kb on - strand at 4262.962 kb on + strand at 4262.962 kb on + strand at 4262.963 kb on - strand at 4262.966 kb on + strand at 4263.088 kb on + strand, within mug at 4263.088 kb on + strand, within mug at 4263.089 kb on - strand, within mug at 4263.090 kb on + strand, within mug at 4263.091 kb on - strand, within mug at 4263.236 kb on + strand, within mug at 4263.301 kb on - strand, within mug at 4263.301 kb on - strand, within mug
Per-strain Table
Position Strand Gene LocusTag Fraction WM_K30 remove 4,261,802 + +0.2 4,261,802 + +0.3 4,261,803 - -0.1 4,261,803 - -3.1 4,261,842 + -1.8 4,261,849 - +0.1 4,261,849 - +0.9 4,261,883 + +0.7 4,261,955 - +0.7 4,262,016 + +0.0 4,262,030 - nfeF JDDGAC_22275 0.11 +0.3 4,262,084 - nfeF JDDGAC_22275 0.18 +1.3 4,262,210 + nfeF JDDGAC_22275 0.34 -1.2 4,262,212 + nfeF JDDGAC_22275 0.34 +0.5 4,262,213 - nfeF JDDGAC_22275 0.35 +0.1 4,262,213 - nfeF JDDGAC_22275 0.35 -0.3 4,262,232 + nfeF JDDGAC_22275 0.37 +0.3 4,262,233 - nfeF JDDGAC_22275 0.37 +0.2 4,262,233 - nfeF JDDGAC_22275 0.37 -0.4 4,262,364 - nfeF JDDGAC_22275 0.54 +0.7 4,262,378 + nfeF JDDGAC_22275 0.56 +0.2 4,262,378 + nfeF JDDGAC_22275 0.56 +0.0 4,262,378 + nfeF JDDGAC_22275 0.56 +0.2 4,262,379 - nfeF JDDGAC_22275 0.56 +0.2 4,262,379 - nfeF JDDGAC_22275 0.56 +0.1 4,262,379 - nfeF JDDGAC_22275 0.56 +0.9 4,262,458 + nfeF JDDGAC_22275 0.67 -0.1 4,262,522 - nfeF JDDGAC_22275 0.75 +1.2 4,262,556 + nfeF JDDGAC_22275 0.79 +0.3 4,262,597 + nfeF JDDGAC_22275 0.85 +0.1 4,262,598 - nfeF JDDGAC_22275 0.85 -1.7 4,262,598 - nfeF JDDGAC_22275 0.85 -0.3 4,262,667 - +0.1 4,262,719 - -0.8 4,262,720 + +0.2 4,262,761 + -3.4 4,262,761 + -0.4 4,262,762 - -0.7 4,262,762 - +0.2 4,262,762 - -0.5 4,262,764 - -1.7 4,262,855 + +0.3 4,262,858 - +0.7 4,262,962 + +0.2 4,262,962 + -0.2 4,262,963 - +0.6 4,262,966 + +0.4 4,263,088 + mug JDDGAC_22285 0.24 -0.4 4,263,088 + mug JDDGAC_22285 0.24 -0.0 4,263,089 - mug JDDGAC_22285 0.24 +0.9 4,263,090 + mug JDDGAC_22285 0.24 +0.8 4,263,091 - mug JDDGAC_22285 0.24 +0.0 4,263,236 + mug JDDGAC_22285 0.53 +0.0 4,263,301 - mug JDDGAC_22285 0.66 -0.4 4,263,301 - mug JDDGAC_22285 0.66 +0.9
Or see this region's nucleotide sequence