Strain Fitness in Escherichia coli ECRC98 around JDDGAC_18940

Experiment: WM_K30

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntescU and etgA overlap by 4 nucleotidesetgA and grlR are separated by 195 nucleotidesgrlR and grlA are separated by 54 nucleotidesgrlA and cesD are separated by 383 nucleotides JDDGAC_18930: escU - type III secretion system LEE export apparatus switch protein EscU, at 3,601,697 to 3,602,734 escU JDDGAC_18935: etgA - type III secretion system LEE muramidase EtgA, at 3,602,731 to 3,603,189 etgA JDDGAC_18940: grlR - type III secretion system LEE GrlA-binding negative regulator GrlR, at 3,603,385 to 3,603,756 grlR JDDGAC_18945: grlA - type III secretion system LEE transcriptional regulator GrlA, at 3,603,811 to 3,604,224 grlA JDDGAC_18950: cesD - SycD/LcrH family type III secretion system chaperone CesD, at 3,604,608 to 3,605,063 cesD Position (kb) 3603 3604Strain fitness (log2 ratio) -1 0 1 2at 3602.449 kb on + strand, within escUat 3602.507 kb on + strand, within escUat 3602.769 kb on - strandat 3603.004 kb on - strand, within etgAat 3603.229 kb on + strandat 3603.407 kb on - strandat 3603.420 kb on + strandat 3603.537 kb on - strand, within grlRat 3603.553 kb on + strand, within grlRat 3603.556 kb on - strand, within grlRat 3603.906 kb on + strand, within grlAat 3604.602 kb on + strandat 3604.602 kb on + strandat 3604.623 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_K30
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3,602,449 + escU JDDGAC_18930 0.72 -0.1
3,602,507 + escU JDDGAC_18930 0.78 +0.3
3,602,769 - +0.2
3,603,004 - etgA JDDGAC_18935 0.59 -1.2
3,603,229 + +2.1
3,603,407 - -0.4
3,603,420 + -0.3
3,603,537 - grlR JDDGAC_18940 0.41 -0.1
3,603,553 + grlR JDDGAC_18940 0.45 -0.4
3,603,556 - grlR JDDGAC_18940 0.46 -0.6
3,603,906 + grlA JDDGAC_18945 0.23 -0.4
3,604,602 + -0.6
3,604,602 + +0.7
3,604,623 + +0.0

Or see this region's nucleotide sequence