Experiment: WM_K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt escU and etgA overlap by 4 nucleotides etgA and grlR are separated by 195 nucleotides grlR and grlA are separated by 54 nucleotides grlA and cesD are separated by 383 nucleotides
JDDGAC_18930: escU - type III secretion system LEE export apparatus switch protein EscU, at 3,601,697 to 3,602,734
escU
JDDGAC_18935: etgA - type III secretion system LEE muramidase EtgA, at 3,602,731 to 3,603,189
etgA
JDDGAC_18940: grlR - type III secretion system LEE GrlA-binding negative regulator GrlR, at 3,603,385 to 3,603,756
grlR
JDDGAC_18945: grlA - type III secretion system LEE transcriptional regulator GrlA, at 3,603,811 to 3,604,224
grlA
JDDGAC_18950: cesD - SycD/LcrH family type III secretion system chaperone CesD, at 3,604,608 to 3,605,063
cesD
Position (kb)
3603
3604 Strain fitness (log2 ratio)
-1
0
1
2 at 3602.449 kb on + strand, within escU at 3602.507 kb on + strand, within escU at 3602.769 kb on - strand at 3603.004 kb on - strand, within etgA at 3603.229 kb on + strand at 3603.407 kb on - strand at 3603.420 kb on + strand at 3603.537 kb on - strand, within grlR at 3603.553 kb on + strand, within grlR at 3603.556 kb on - strand, within grlR at 3603.906 kb on + strand, within grlA at 3604.602 kb on + strand at 3604.602 kb on + strand at 3604.623 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_K30 remove 3,602,449 + escU JDDGAC_18930 0.72 -0.1 3,602,507 + escU JDDGAC_18930 0.78 +0.3 3,602,769 - +0.2 3,603,004 - etgA JDDGAC_18935 0.59 -1.2 3,603,229 + +2.1 3,603,407 - -0.4 3,603,420 + -0.3 3,603,537 - grlR JDDGAC_18940 0.41 -0.1 3,603,553 + grlR JDDGAC_18940 0.45 -0.4 3,603,556 - grlR JDDGAC_18940 0.46 -0.6 3,603,906 + grlA JDDGAC_18945 0.23 -0.4 3,604,602 + -0.6 3,604,602 + +0.7 3,604,623 + +0.0
Or see this region's nucleotide sequence