Experiment: WM_K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ddlA and yaiZ are separated by 23 nucleotides yaiZ and yaiY are separated by 259 nucleotides yaiY and yaiW are separated by 58 nucleotides
JDDGAC_12290: ddlA - D-alanine--D-alanine ligase, at 2,264,237 to 2,265,331
ddlA
JDDGAC_12295: yaiZ - Uncharacterized protein YaiZ, at 2,265,355 to 2,265,567
yaiZ
JDDGAC_12300: yaiY - Inner membrane protein YaiY, at 2,265,827 to 2,266,135
yaiY
JDDGAC_12305: yaiW - surface-exposed outer membrane lipoprotein YaiW, at 2,266,194 to 2,267,288
yaiW
Position (kb)
2265
2266 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2264.412 kb on + strand, within ddlA at 2264.413 kb on - strand, within ddlA at 2264.435 kb on - strand, within ddlA at 2264.620 kb on - strand, within ddlA at 2264.650 kb on + strand, within ddlA at 2264.651 kb on - strand, within ddlA at 2264.710 kb on + strand, within ddlA at 2264.711 kb on - strand, within ddlA at 2264.711 kb on - strand, within ddlA at 2264.711 kb on - strand, within ddlA at 2264.764 kb on + strand, within ddlA at 2264.884 kb on + strand, within ddlA at 2264.996 kb on + strand, within ddlA at 2265.002 kb on + strand, within ddlA at 2265.002 kb on + strand, within ddlA at 2265.014 kb on + strand, within ddlA at 2265.015 kb on - strand, within ddlA at 2265.015 kb on - strand, within ddlA at 2265.076 kb on + strand, within ddlA at 2265.077 kb on - strand, within ddlA at 2265.214 kb on + strand, within ddlA at 2265.214 kb on + strand, within ddlA at 2265.214 kb on + strand, within ddlA at 2265.214 kb on + strand, within ddlA at 2265.214 kb on + strand, within ddlA at 2265.215 kb on - strand, within ddlA at 2265.215 kb on - strand, within ddlA at 2265.251 kb on + strand at 2265.252 kb on - strand at 2265.329 kb on + strand at 2265.329 kb on + strand at 2265.330 kb on - strand at 2265.337 kb on + strand at 2265.351 kb on - strand at 2265.351 kb on - strand at 2265.393 kb on + strand, within yaiZ at 2265.446 kb on + strand, within yaiZ at 2265.495 kb on + strand, within yaiZ at 2265.495 kb on + strand, within yaiZ at 2265.505 kb on + strand, within yaiZ at 2265.507 kb on + strand, within yaiZ at 2265.507 kb on + strand, within yaiZ at 2265.507 kb on + strand, within yaiZ at 2265.508 kb on - strand, within yaiZ at 2265.527 kb on + strand, within yaiZ at 2265.528 kb on - strand, within yaiZ at 2265.528 kb on - strand, within yaiZ at 2265.530 kb on + strand, within yaiZ at 2265.531 kb on - strand, within yaiZ at 2265.531 kb on - strand, within yaiZ at 2265.602 kb on + strand at 2265.710 kb on + strand at 2265.728 kb on - strand at 2265.826 kb on + strand at 2265.826 kb on + strand at 2265.961 kb on - strand, within yaiY at 2265.981 kb on - strand, within yaiY at 2266.036 kb on - strand, within yaiY at 2266.074 kb on + strand, within yaiY at 2266.104 kb on + strand, within yaiY at 2266.104 kb on + strand, within yaiY at 2266.140 kb on + strand at 2266.159 kb on + strand at 2266.169 kb on + strand at 2266.205 kb on + strand at 2266.205 kb on + strand at 2266.205 kb on + strand at 2266.320 kb on - strand, within yaiW at 2266.357 kb on + strand, within yaiW at 2266.358 kb on - strand, within yaiW at 2266.398 kb on + strand, within yaiW at 2266.481 kb on + strand, within yaiW at 2266.481 kb on + strand, within yaiW
Per-strain Table
Position Strand Gene LocusTag Fraction WM_K30 remove 2,264,412 + ddlA JDDGAC_12290 0.16 +1.0 2,264,413 - ddlA JDDGAC_12290 0.16 -0.2 2,264,435 - ddlA JDDGAC_12290 0.18 +1.3 2,264,620 - ddlA JDDGAC_12290 0.35 -0.2 2,264,650 + ddlA JDDGAC_12290 0.38 -0.1 2,264,651 - ddlA JDDGAC_12290 0.38 +0.1 2,264,710 + ddlA JDDGAC_12290 0.43 +2.3 2,264,711 - ddlA JDDGAC_12290 0.43 -0.3 2,264,711 - ddlA JDDGAC_12290 0.43 +0.3 2,264,711 - ddlA JDDGAC_12290 0.43 +0.3 2,264,764 + ddlA JDDGAC_12290 0.48 -1.8 2,264,884 + ddlA JDDGAC_12290 0.59 -0.7 2,264,996 + ddlA JDDGAC_12290 0.69 +1.4 2,265,002 + ddlA JDDGAC_12290 0.70 +0.3 2,265,002 + ddlA JDDGAC_12290 0.70 -0.4 2,265,014 + ddlA JDDGAC_12290 0.71 -0.0 2,265,015 - ddlA JDDGAC_12290 0.71 -1.6 2,265,015 - ddlA JDDGAC_12290 0.71 +0.2 2,265,076 + ddlA JDDGAC_12290 0.77 +0.3 2,265,077 - ddlA JDDGAC_12290 0.77 -0.7 2,265,214 + ddlA JDDGAC_12290 0.89 +0.6 2,265,214 + ddlA JDDGAC_12290 0.89 -0.1 2,265,214 + ddlA JDDGAC_12290 0.89 -0.0 2,265,214 + ddlA JDDGAC_12290 0.89 -0.3 2,265,214 + ddlA JDDGAC_12290 0.89 +0.0 2,265,215 - ddlA JDDGAC_12290 0.89 +0.0 2,265,215 - ddlA JDDGAC_12290 0.89 -0.4 2,265,251 + -0.5 2,265,252 - +0.6 2,265,329 + -0.3 2,265,329 + -0.5 2,265,330 - -2.7 2,265,337 + +1.2 2,265,351 - +0.3 2,265,351 - -0.1 2,265,393 + yaiZ JDDGAC_12295 0.18 +0.7 2,265,446 + yaiZ JDDGAC_12295 0.43 -0.2 2,265,495 + yaiZ JDDGAC_12295 0.66 +0.8 2,265,495 + yaiZ JDDGAC_12295 0.66 +0.4 2,265,505 + yaiZ JDDGAC_12295 0.70 +0.8 2,265,507 + yaiZ JDDGAC_12295 0.71 +2.5 2,265,507 + yaiZ JDDGAC_12295 0.71 +0.7 2,265,507 + yaiZ JDDGAC_12295 0.71 +0.2 2,265,508 - yaiZ JDDGAC_12295 0.72 -0.3 2,265,527 + yaiZ JDDGAC_12295 0.81 -0.3 2,265,528 - yaiZ JDDGAC_12295 0.81 -0.4 2,265,528 - yaiZ JDDGAC_12295 0.81 +0.4 2,265,530 + yaiZ JDDGAC_12295 0.82 +0.2 2,265,531 - yaiZ JDDGAC_12295 0.83 -1.2 2,265,531 - yaiZ JDDGAC_12295 0.83 -0.5 2,265,602 + +1.6 2,265,710 + -0.3 2,265,728 - -0.0 2,265,826 + +0.4 2,265,826 + +1.8 2,265,961 - yaiY JDDGAC_12300 0.43 -0.7 2,265,981 - yaiY JDDGAC_12300 0.50 +0.3 2,266,036 - yaiY JDDGAC_12300 0.68 +2.3 2,266,074 + yaiY JDDGAC_12300 0.80 -0.4 2,266,104 + yaiY JDDGAC_12300 0.90 +0.1 2,266,104 + yaiY JDDGAC_12300 0.90 +0.9 2,266,140 + +0.7 2,266,159 + -2.8 2,266,169 + +0.2 2,266,205 + -1.1 2,266,205 + -0.2 2,266,205 + -1.2 2,266,320 - yaiW JDDGAC_12305 0.12 -0.1 2,266,357 + yaiW JDDGAC_12305 0.15 +0.2 2,266,358 - yaiW JDDGAC_12305 0.15 -1.2 2,266,398 + yaiW JDDGAC_12305 0.19 -0.1 2,266,481 + yaiW JDDGAC_12305 0.26 -1.1 2,266,481 + yaiW JDDGAC_12305 0.26 +0.8
Or see this region's nucleotide sequence