Experiment: WM_K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt JDDGAC_10220 and JDDGAC_10225 are separated by 621 nucleotides JDDGAC_10225 and JDDGAC_10230 are separated by 99 nucleotides JDDGAC_10230 and JDDGAC_10235 overlap by 62 nucleotides JDDGAC_10235 and JDDGAC_10240 are separated by 183 nucleotides
JDDGAC_10220: JDDGAC_10220 - holin, at 1,823,293 to 1,823,508
_10220
JDDGAC_10225: JDDGAC_10225 - DUF3927 domain-containing protein, at 1,824,130 to 1,824,210
_10225
JDDGAC_10230: JDDGAC_10230 - hypothetical protein, at 1,824,310 to 1,824,435
_10230
JDDGAC_10235: JDDGAC_10235 - Membrane protein, at 1,824,374 to 1,825,117
_10235
JDDGAC_10240: JDDGAC_10240 - antitermination protein, at 1,825,301 to 1,825,990
_10240
Position (kb)
1824
1825 Strain fitness (log2 ratio)
-2
-1
0
1 at 1823.339 kb on + strand, within JDDGAC_10220 at 1823.354 kb on + strand, within JDDGAC_10220 at 1823.379 kb on + strand, within JDDGAC_10220 at 1823.505 kb on - strand at 1823.505 kb on - strand at 1823.582 kb on - strand at 1823.593 kb on + strand at 1823.604 kb on + strand at 1823.604 kb on + strand at 1823.617 kb on - strand at 1823.629 kb on + strand at 1823.631 kb on + strand at 1823.632 kb on - strand at 1823.655 kb on + strand at 1823.656 kb on - strand at 1823.656 kb on - strand at 1823.659 kb on + strand at 1823.659 kb on + strand at 1823.660 kb on - strand at 1823.753 kb on - strand at 1823.787 kb on - strand at 1823.964 kb on + strand at 1823.988 kb on + strand at 1823.988 kb on + strand at 1823.989 kb on - strand at 1824.167 kb on - strand, within JDDGAC_10225 at 1825.184 kb on + strand at 1825.184 kb on + strand at 1825.188 kb on + strand at 1825.188 kb on + strand at 1825.188 kb on + strand at 1825.210 kb on - strand at 1825.216 kb on - strand at 1825.257 kb on + strand at 1825.273 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_K30 remove 1,823,339 + JDDGAC_10220 0.21 +0.7 1,823,354 + JDDGAC_10220 0.28 +0.2 1,823,379 + JDDGAC_10220 0.40 -0.9 1,823,505 - +0.2 1,823,505 - +0.2 1,823,582 - -0.8 1,823,593 + -0.5 1,823,604 + +1.2 1,823,604 + +0.7 1,823,617 - -0.2 1,823,629 + -0.5 1,823,631 + -0.2 1,823,632 - +0.0 1,823,655 + -0.0 1,823,656 - -0.0 1,823,656 - -0.0 1,823,659 + -0.0 1,823,659 + +0.9 1,823,660 - -0.6 1,823,753 - +0.3 1,823,787 - -1.8 1,823,964 + +0.5 1,823,988 + +0.7 1,823,988 + -0.9 1,823,989 - -2.7 1,824,167 - JDDGAC_10225 0.46 -0.9 1,825,184 + +1.6 1,825,184 + -0.0 1,825,188 + -1.1 1,825,188 + -0.3 1,825,188 + -0.4 1,825,210 - +0.0 1,825,216 - +0.3 1,825,257 + +0.1 1,825,273 + -0.3
Or see this region's nucleotide sequence