Experiment: WM_K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt JDDGAC_00475 and cysE are separated by 156 nucleotides cysE and gpA1 are separated by 43 nucleotides gpA1 and JDDGAC_00495 are separated by 170 nucleotides JDDGAC_00495 and tnp-IS3 overlap by 1 nucleotides
JDDGAC_00475: JDDGAC_00475 - oxidoreductase, at 82,777 to 83,265
_00475
JDDGAC_00480: cysE - serine acetyltransferase, at 83,422 to 83,994
cysE
JDDGAC_00485: gpA1 - Phage terminase, large subunit GpA, at 84,038 to 84,454
gpA1
JDDGAC_00495: JDDGAC_00495 - Insertion element IS629 uncharacterized protein, at 84,625 to 84,951
_00495
JDDGAC_00500: tnp-IS3 - IS3 family transposase, at 84,951 to 85,640
tnp-IS3
Position (kb) 84
85 Strain fitness (log2 ratio) -2
-1
0
1
2 at 83.054 kb on - strand, within JDDGAC_00475 at 83.054 kb on - strand, within JDDGAC_00475 at 83.054 kb on - strand, within JDDGAC_00475 at 83.191 kb on - strand, within JDDGAC_00475 at 83.212 kb on - strand, within JDDGAC_00475 at 83.233 kb on - strand at 83.269 kb on - strand at 83.273 kb on + strand at 83.382 kb on - strand at 83.384 kb on - strand at 83.406 kb on - strand at 83.431 kb on + strand at 83.436 kb on - strand at 83.518 kb on - strand, within cysE at 83.604 kb on + strand, within cysE at 83.801 kb on + strand, within cysE at 83.811 kb on + strand, within cysE at 84.001 kb on + strand at 84.082 kb on + strand, within gpA1 at 84.188 kb on - strand, within gpA1 at 84.553 kb on + strand at 84.598 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_K30 remove 83,054 - JDDGAC_00475 0.57 +0.1 83,054 - JDDGAC_00475 0.57 +0.1 83,054 - JDDGAC_00475 0.57 -0.2 83,191 - JDDGAC_00475 0.85 -0.6 83,212 - JDDGAC_00475 0.89 +0.5 83,233 - -0.8 83,269 - +0.3 83,273 + -1.1 83,382 - +0.8 83,384 - +2.5 83,406 - +0.5 83,431 + -0.1 83,436 - -0.9 83,518 - cysE JDDGAC_00480 0.17 -0.0 83,604 + cysE JDDGAC_00480 0.32 -0.7 83,801 + cysE JDDGAC_00480 0.66 -0.1 83,811 + cysE JDDGAC_00480 0.68 +0.9 84,001 + +0.2 84,082 + gpA1 JDDGAC_00485 0.11 -1.8 84,188 - gpA1 JDDGAC_00485 0.36 -0.8 84,553 + +0.3 84,598 - +0.5
Or see this region's nucleotide sequence