Strain Fitness in Escherichia coli ECRC98 around JDDGAC_28450

Experiment: WM_EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaD and wcaE are separated by 10 nucleotideswcaE and wcaF are separated by 15 nucleotideswcaF and JDDGAC_28455 are separated by 28 nucleotides JDDGAC_28440: wcaD - putative colanic acid polymerase WcaD, at 5,452,983 to 5,454,200 wcaD JDDGAC_28445: wcaE - colanic acid biosynthesis glycosyltransferase WcaE, at 5,454,211 to 5,454,957 wcaE JDDGAC_28450: wcaF - colanic acid biosynthesis acetyltransferase WcaF, at 5,454,973 to 5,455,521 wcaF JDDGAC_28455: JDDGAC_28455 - GDP-mannose 4,6-dehydratase, at 5,455,550 to 5,456,671 _28455 Position (kb) 5454 5455 5456Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 5454.390 kb on - strand, within wcaEat 5454.390 kb on - strand, within wcaEat 5454.390 kb on - strand, within wcaEat 5455.030 kb on - strand, within wcaFat 5455.085 kb on + strand, within wcaFat 5455.112 kb on + strand, within wcaFat 5455.289 kb on + strand, within wcaFat 5455.289 kb on + strand, within wcaFat 5455.544 kb on + strandat 5455.578 kb on + strandat 5455.643 kb on - strandat 5455.643 kb on - strandat 5455.645 kb on + strandat 5455.645 kb on + strandat 5455.646 kb on - strandat 5455.751 kb on - strand, within JDDGAC_28455at 5455.768 kb on + strand, within JDDGAC_28455at 5455.800 kb on - strand, within JDDGAC_28455at 5455.876 kb on + strand, within JDDGAC_28455at 5456.037 kb on - strand, within JDDGAC_28455at 5456.072 kb on + strand, within JDDGAC_28455at 5456.081 kb on + strand, within JDDGAC_28455at 5456.081 kb on + strand, within JDDGAC_28455at 5456.082 kb on - strand, within JDDGAC_28455at 5456.082 kb on - strand, within JDDGAC_28455at 5456.082 kb on - strand, within JDDGAC_28455at 5456.082 kb on - strand, within JDDGAC_28455at 5456.082 kb on - strand, within JDDGAC_28455at 5456.095 kb on + strand, within JDDGAC_28455at 5456.209 kb on + strand, within JDDGAC_28455at 5456.319 kb on - strand, within JDDGAC_28455at 5456.337 kb on + strand, within JDDGAC_28455

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_EV240
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5,454,390 - wcaE JDDGAC_28445 0.24 -1.6
5,454,390 - wcaE JDDGAC_28445 0.24 -2.0
5,454,390 - wcaE JDDGAC_28445 0.24 +0.7
5,455,030 - wcaF JDDGAC_28450 0.10 -1.8
5,455,085 + wcaF JDDGAC_28450 0.20 -1.5
5,455,112 + wcaF JDDGAC_28450 0.25 -0.5
5,455,289 + wcaF JDDGAC_28450 0.58 -3.9
5,455,289 + wcaF JDDGAC_28450 0.58 -3.4
5,455,544 + +0.1
5,455,578 + -2.4
5,455,643 - +0.3
5,455,643 - -1.6
5,455,645 + -0.4
5,455,645 + +1.2
5,455,646 - +1.8
5,455,751 - JDDGAC_28455 0.18 +0.7
5,455,768 + JDDGAC_28455 0.19 +1.2
5,455,800 - JDDGAC_28455 0.22 -1.1
5,455,876 + JDDGAC_28455 0.29 -1.2
5,456,037 - JDDGAC_28455 0.43 -0.3
5,456,072 + JDDGAC_28455 0.47 +0.2
5,456,081 + JDDGAC_28455 0.47 +0.4
5,456,081 + JDDGAC_28455 0.47 -0.1
5,456,082 - JDDGAC_28455 0.47 -1.7
5,456,082 - JDDGAC_28455 0.47 +0.6
5,456,082 - JDDGAC_28455 0.47 +0.6
5,456,082 - JDDGAC_28455 0.47 -0.2
5,456,082 - JDDGAC_28455 0.47 -2.1
5,456,095 + JDDGAC_28455 0.49 +1.5
5,456,209 + JDDGAC_28455 0.59 +0.8
5,456,319 - JDDGAC_28455 0.69 -0.1
5,456,337 + JDDGAC_28455 0.70 +1.1

Or see this region's nucleotide sequence