Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yibN and grxC are separated by 141 nucleotides grxC and secB are separated by 62 nucleotides secB and gpsA overlap by 1 nucleotides gpsA and cysE are separated by 79 nucleotides cysE and trmL are separated by 52 nucleotides
JDDGAC_19365: yibN - Uncharacterized protein YibN, at 3,686,162 to 3,686,593
yibN
JDDGAC_19370: grxC - glutaredoxin 3, at 3,686,735 to 3,686,986
grxC
JDDGAC_19375: secB - protein-export chaperone SecB, at 3,687,049 to 3,687,516
secB
JDDGAC_19380: gpsA - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 3,687,516 to 3,688,535
gpsA
JDDGAC_19385: cysE - serine O-acetyltransferase, at 3,688,615 to 3,689,436
cysE
JDDGAC_19390: trmL - tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine (34)-2'-O)-methyltransferase TrmL, at 3,689,489 to 3,689,962
trmL
Position (kb)
3687
3688
3689 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3686.526 kb on + strand, within yibN at 3686.591 kb on + strand at 3686.592 kb on - strand at 3686.625 kb on + strand at 3686.635 kb on + strand at 3686.636 kb on - strand at 3686.636 kb on - strand at 3686.636 kb on - strand at 3686.651 kb on + strand at 3686.651 kb on + strand at 3686.652 kb on - strand at 3686.652 kb on - strand at 3686.657 kb on + strand at 3686.657 kb on + strand at 3686.659 kb on + strand at 3686.755 kb on + strand at 3687.004 kb on + strand at 3687.004 kb on + strand at 3687.016 kb on + strand at 3687.020 kb on + strand at 3687.033 kb on + strand at 3687.466 kb on + strand, within secB at 3688.592 kb on + strand at 3688.592 kb on + strand at 3688.592 kb on + strand at 3688.593 kb on - strand at 3688.593 kb on - strand at 3689.340 kb on + strand, within cysE at 3689.400 kb on + strand at 3689.403 kb on + strand at 3689.410 kb on - strand at 3689.434 kb on + strand at 3689.434 kb on + strand at 3689.434 kb on + strand at 3689.435 kb on - strand at 3689.435 kb on - strand at 3689.435 kb on - strand at 3689.435 kb on - strand at 3689.435 kb on - strand at 3689.435 kb on - strand at 3689.435 kb on - strand at 3689.435 kb on - strand at 3689.464 kb on + strand at 3689.465 kb on - strand at 3689.465 kb on - strand at 3689.472 kb on + strand at 3689.481 kb on + strand at 3689.481 kb on + strand at 3689.482 kb on - strand at 3689.486 kb on + strand at 3689.486 kb on + strand at 3689.486 kb on + strand at 3689.487 kb on - strand at 3689.490 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 3,686,526 + yibN JDDGAC_19365 0.84 +0.7 3,686,591 + -0.7 3,686,592 - -2.8 3,686,625 + +0.6 3,686,635 + +0.9 3,686,636 - +1.1 3,686,636 - -0.0 3,686,636 - +1.2 3,686,651 + -4.5 3,686,651 + -0.4 3,686,652 - -0.9 3,686,652 - -0.8 3,686,657 + -0.5 3,686,657 + +2.1 3,686,659 + -2.9 3,686,755 + +1.9 3,687,004 + +1.4 3,687,004 + -0.3 3,687,016 + -0.5 3,687,020 + +0.9 3,687,033 + -1.0 3,687,466 + secB JDDGAC_19375 0.89 -1.8 3,688,592 + -1.1 3,688,592 + -0.0 3,688,592 + +0.8 3,688,593 - -2.2 3,688,593 - -1.1 3,689,340 + cysE JDDGAC_19385 0.88 -0.4 3,689,400 + +0.3 3,689,403 + -0.3 3,689,410 - +0.0 3,689,434 + -0.8 3,689,434 + -2.4 3,689,434 + -1.3 3,689,435 - -2.2 3,689,435 - -0.1 3,689,435 - +0.1 3,689,435 - -2.5 3,689,435 - +2.6 3,689,435 - -2.0 3,689,435 - +1.0 3,689,435 - +1.4 3,689,464 + -1.8 3,689,465 - +0.7 3,689,465 - +0.1 3,689,472 + +0.4 3,689,481 + -0.0 3,689,481 + -0.8 3,689,482 - -0.6 3,689,486 + -2.1 3,689,486 + -2.1 3,689,486 + +0.2 3,689,487 - +0.2 3,689,490 + +1.0
Or see this region's nucleotide sequence