Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ubiD and rfaH are separated by 166 nucleotides rfaH and tatD overlap by 4 nucleotides tatD and tatC are separated by 41 nucleotides tatC and tatB are separated by 2 nucleotides
JDDGAC_17885: ubiD - 4-hydroxy-3-polyprenylbenzoate decarboxylase, at 3,395,187 to 3,396,680
ubiD
JDDGAC_17890: rfaH - transcription/translation regulatory transformer protein RfaH, at 3,396,847 to 3,397,335
rfaH
JDDGAC_17895: tatD - 3'-5' ssDNA/RNA exonuclease TatD, at 3,397,332 to 3,398,114
tatD
JDDGAC_17900: tatC - Sec-independent protein translocase subunit TatC, at 3,398,156 to 3,398,932
tatC
JDDGAC_17905: tatB - Sec-independent protein translocase protein TatB, at 3,398,935 to 3,399,450
tatB
Position (kb)
3397
3398
3399 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 3396.665 kb on - strand at 3396.692 kb on - strand at 3396.708 kb on - strand at 3396.747 kb on - strand at 3396.765 kb on - strand at 3396.765 kb on - strand at 3396.766 kb on + strand at 3396.766 kb on + strand at 3396.766 kb on + strand at 3396.783 kb on + strand at 3396.785 kb on + strand at 3396.785 kb on + strand at 3396.785 kb on + strand at 3396.786 kb on - strand at 3396.786 kb on - strand at 3396.786 kb on - strand at 3396.786 kb on - strand at 3396.786 kb on - strand at 3396.786 kb on - strand at 3396.789 kb on + strand at 3396.789 kb on + strand at 3396.789 kb on + strand at 3396.790 kb on - strand at 3396.812 kb on - strand at 3397.276 kb on - strand, within rfaH at 3397.276 kb on - strand, within rfaH at 3397.284 kb on + strand, within rfaH at 3397.285 kb on - strand, within rfaH at 3397.296 kb on + strand at 3397.296 kb on + strand at 3397.297 kb on - strand at 3397.297 kb on - strand at 3397.365 kb on - strand at 3397.418 kb on + strand, within tatD at 3397.418 kb on + strand, within tatD at 3397.418 kb on + strand, within tatD at 3397.419 kb on - strand, within tatD at 3397.419 kb on - strand, within tatD at 3397.419 kb on - strand, within tatD at 3397.419 kb on - strand, within tatD at 3397.419 kb on - strand, within tatD at 3397.419 kb on - strand, within tatD at 3397.419 kb on - strand, within tatD at 3397.431 kb on - strand, within tatD at 3397.606 kb on + strand, within tatD at 3397.607 kb on - strand, within tatD at 3397.701 kb on + strand, within tatD at 3397.709 kb on + strand, within tatD at 3397.739 kb on + strand, within tatD at 3397.739 kb on + strand, within tatD at 3397.739 kb on + strand, within tatD at 3397.740 kb on - strand, within tatD at 3397.740 kb on - strand, within tatD at 3397.740 kb on - strand, within tatD at 3397.740 kb on - strand, within tatD at 3397.756 kb on - strand, within tatD at 3397.756 kb on - strand, within tatD at 3397.773 kb on + strand, within tatD at 3397.773 kb on + strand, within tatD at 3397.774 kb on - strand, within tatD at 3397.774 kb on - strand, within tatD at 3397.774 kb on - strand, within tatD at 3397.933 kb on - strand, within tatD at 3397.933 kb on - strand, within tatD at 3398.028 kb on - strand, within tatD at 3398.119 kb on + strand at 3398.126 kb on + strand at 3398.127 kb on - strand at 3398.127 kb on - strand at 3398.127 kb on - strand at 3398.127 kb on - strand at 3398.158 kb on - strand at 3398.226 kb on + strand at 3398.349 kb on + strand, within tatC at 3398.349 kb on + strand, within tatC at 3398.383 kb on + strand, within tatC at 3398.472 kb on + strand, within tatC at 3398.472 kb on + strand, within tatC at 3398.583 kb on - strand, within tatC at 3398.650 kb on + strand, within tatC at 3398.809 kb on - strand, within tatC at 3398.809 kb on - strand, within tatC at 3398.943 kb on + strand at 3399.096 kb on + strand, within tatB at 3399.097 kb on - strand, within tatB
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 3,396,665 - -3.1 3,396,692 - +0.7 3,396,708 - -2.7 3,396,747 - +0.4 3,396,765 - +0.8 3,396,765 - +1.0 3,396,766 + +1.3 3,396,766 + +2.9 3,396,766 + +2.0 3,396,783 + +1.8 3,396,785 + +0.7 3,396,785 + +1.4 3,396,785 + +0.8 3,396,786 - -1.7 3,396,786 - -3.9 3,396,786 - -1.3 3,396,786 - -2.9 3,396,786 - -3.8 3,396,786 - -3.3 3,396,789 + +3.5 3,396,789 + +2.1 3,396,789 + +2.0 3,396,790 - +1.1 3,396,812 - -4.8 3,397,276 - rfaH JDDGAC_17890 0.88 -2.9 3,397,276 - rfaH JDDGAC_17890 0.88 -3.8 3,397,284 + rfaH JDDGAC_17890 0.89 -1.3 3,397,285 - rfaH JDDGAC_17890 0.90 -4.1 3,397,296 + -3.3 3,397,296 + -4.9 3,397,297 - -4.8 3,397,297 - -2.9 3,397,365 - -0.5 3,397,418 + tatD JDDGAC_17895 0.11 -0.1 3,397,418 + tatD JDDGAC_17895 0.11 -1.4 3,397,418 + tatD JDDGAC_17895 0.11 -0.5 3,397,419 - tatD JDDGAC_17895 0.11 -0.3 3,397,419 - tatD JDDGAC_17895 0.11 -0.6 3,397,419 - tatD JDDGAC_17895 0.11 -3.0 3,397,419 - tatD JDDGAC_17895 0.11 -0.0 3,397,419 - tatD JDDGAC_17895 0.11 -2.1 3,397,419 - tatD JDDGAC_17895 0.11 +0.8 3,397,419 - tatD JDDGAC_17895 0.11 -0.0 3,397,431 - tatD JDDGAC_17895 0.13 -0.4 3,397,606 + tatD JDDGAC_17895 0.35 +1.9 3,397,607 - tatD JDDGAC_17895 0.35 +1.1 3,397,701 + tatD JDDGAC_17895 0.47 -0.3 3,397,709 + tatD JDDGAC_17895 0.48 -2.4 3,397,739 + tatD JDDGAC_17895 0.52 -0.6 3,397,739 + tatD JDDGAC_17895 0.52 -0.4 3,397,739 + tatD JDDGAC_17895 0.52 +0.6 3,397,740 - tatD JDDGAC_17895 0.52 +1.1 3,397,740 - tatD JDDGAC_17895 0.52 +0.8 3,397,740 - tatD JDDGAC_17895 0.52 -1.4 3,397,740 - tatD JDDGAC_17895 0.52 +0.4 3,397,756 - tatD JDDGAC_17895 0.54 +0.3 3,397,756 - tatD JDDGAC_17895 0.54 +0.3 3,397,773 + tatD JDDGAC_17895 0.56 -0.2 3,397,773 + tatD JDDGAC_17895 0.56 -0.5 3,397,774 - tatD JDDGAC_17895 0.56 +0.9 3,397,774 - tatD JDDGAC_17895 0.56 -0.6 3,397,774 - tatD JDDGAC_17895 0.56 -2.7 3,397,933 - tatD JDDGAC_17895 0.77 +1.3 3,397,933 - tatD JDDGAC_17895 0.77 +1.6 3,398,028 - tatD JDDGAC_17895 0.89 +0.5 3,398,119 + +0.9 3,398,126 + +0.7 3,398,127 - -0.1 3,398,127 - -0.2 3,398,127 - -0.4 3,398,127 - +0.8 3,398,158 - +0.0 3,398,226 + -0.1 3,398,349 + tatC JDDGAC_17900 0.25 +1.1 3,398,349 + tatC JDDGAC_17900 0.25 +0.7 3,398,383 + tatC JDDGAC_17900 0.29 -0.7 3,398,472 + tatC JDDGAC_17900 0.41 -1.3 3,398,472 + tatC JDDGAC_17900 0.41 +1.5 3,398,583 - tatC JDDGAC_17900 0.55 +1.9 3,398,650 + tatC JDDGAC_17900 0.64 +0.5 3,398,809 - tatC JDDGAC_17900 0.84 -0.4 3,398,809 - tatC JDDGAC_17900 0.84 +2.9 3,398,943 + +0.9 3,399,096 + tatB JDDGAC_17905 0.31 +0.7 3,399,097 - tatB JDDGAC_17905 0.31 -0.9
Or see this region's nucleotide sequence