Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yciV and trpL are separated by 137 nucleotides trpL and trpE are separated by 91 nucleotides trpE and trpD overlap by 1 nucleotides
JDDGAC_05190: yciV - 5'-3' exoribonuclease, at 912,788 to 913,669
yciV
JDDGAC_05195: trpL - trp operon leader peptide, at 913,807 to 913,851
trpL
JDDGAC_05200: trpE - anthranilate synthase component I, at 913,943 to 915,505
trpE
JDDGAC_05205: trpD - bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD, at 915,505 to 917,100
trpD
Position (kb) 913
914
915
916 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 913.052 kb on - strand, within yciV at 913.083 kb on - strand, within yciV at 913.083 kb on - strand, within yciV at 913.083 kb on - strand, within yciV at 913.187 kb on - strand, within yciV at 913.302 kb on - strand, within yciV at 913.302 kb on - strand, within yciV at 913.397 kb on - strand, within yciV at 913.653 kb on + strand at 913.654 kb on - strand at 913.728 kb on + strand at 913.938 kb on + strand at 913.938 kb on + strand at 913.938 kb on + strand at 914.068 kb on + strand at 914.126 kb on - strand, within trpE at 914.191 kb on - strand, within trpE at 914.396 kb on + strand, within trpE at 914.396 kb on + strand, within trpE at 914.397 kb on - strand, within trpE at 914.786 kb on + strand, within trpE at 914.816 kb on + strand, within trpE at 914.816 kb on + strand, within trpE at 914.817 kb on - strand, within trpE at 914.817 kb on - strand, within trpE at 914.882 kb on + strand, within trpE at 914.882 kb on + strand, within trpE at 915.125 kb on + strand, within trpE at 915.126 kb on - strand, within trpE at 915.202 kb on + strand, within trpE at 915.238 kb on - strand, within trpE at 915.253 kb on - strand, within trpE at 915.543 kb on + strand at 915.544 kb on - strand at 915.548 kb on - strand at 915.548 kb on - strand at 915.582 kb on + strand at 915.582 kb on + strand at 915.595 kb on + strand at 915.607 kb on - strand at 915.623 kb on + strand at 915.703 kb on - strand, within trpD at 915.703 kb on - strand, within trpD at 915.918 kb on + strand, within trpD at 916.126 kb on + strand, within trpD at 916.126 kb on + strand, within trpD at 916.482 kb on + strand, within trpD at 916.485 kb on + strand, within trpD
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 913,052 - yciV JDDGAC_05190 0.30 +0.9 913,083 - yciV JDDGAC_05190 0.33 -1.0 913,083 - yciV JDDGAC_05190 0.33 +1.0 913,083 - yciV JDDGAC_05190 0.33 -1.4 913,187 - yciV JDDGAC_05190 0.45 -2.7 913,302 - yciV JDDGAC_05190 0.58 +0.1 913,302 - yciV JDDGAC_05190 0.58 +3.4 913,397 - yciV JDDGAC_05190 0.69 +0.9 913,653 + -2.0 913,654 - -1.2 913,728 + +0.9 913,938 + +1.8 913,938 + +2.0 913,938 + +0.5 914,068 + -0.4 914,126 - trpE JDDGAC_05200 0.12 +1.5 914,191 - trpE JDDGAC_05200 0.16 -1.2 914,396 + trpE JDDGAC_05200 0.29 +2.0 914,396 + trpE JDDGAC_05200 0.29 +1.4 914,397 - trpE JDDGAC_05200 0.29 +0.9 914,786 + trpE JDDGAC_05200 0.54 +0.7 914,816 + trpE JDDGAC_05200 0.56 +1.0 914,816 + trpE JDDGAC_05200 0.56 +1.0 914,817 - trpE JDDGAC_05200 0.56 -5.0 914,817 - trpE JDDGAC_05200 0.56 -0.6 914,882 + trpE JDDGAC_05200 0.60 -1.4 914,882 + trpE JDDGAC_05200 0.60 +1.1 915,125 + trpE JDDGAC_05200 0.76 +0.2 915,126 - trpE JDDGAC_05200 0.76 +0.7 915,202 + trpE JDDGAC_05200 0.81 +1.5 915,238 - trpE JDDGAC_05200 0.83 +1.9 915,253 - trpE JDDGAC_05200 0.84 +1.7 915,543 + -0.2 915,544 - +0.5 915,548 - +1.8 915,548 - +1.4 915,582 + +2.2 915,582 + -2.5 915,595 + -0.4 915,607 - -0.8 915,623 + +1.0 915,703 - trpD JDDGAC_05205 0.12 +0.0 915,703 - trpD JDDGAC_05205 0.12 +1.5 915,918 + trpD JDDGAC_05205 0.26 +0.8 916,126 + trpD JDDGAC_05205 0.39 -2.4 916,126 + trpD JDDGAC_05205 0.39 +1.9 916,482 + trpD JDDGAC_05205 0.61 -0.8 916,485 + trpD JDDGAC_05205 0.61 -2.2
Or see this region's nucleotide sequence