Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gGDEF and uxaB are separated by 226 nucleotides uxaB and tam are separated by 277 nucleotides tam and lsrG are separated by 56 nucleotides lsrG and lsrF are separated by 23 nucleotides
JDDGAC_03635: gGDEF - diguanylate cyclase, at 604,287 to 605,705
gGDEF
JDDGAC_03640: uxaB - tagaturonate reductase, at 605,932 to 607,383
uxaB
JDDGAC_03645: tam - trans-aconitate 2-methyltransferase, at 607,661 to 607,927
tam
JDDGAC_03650: lsrG - (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase, at 607,984 to 608,274
lsrG
JDDGAC_03655: lsrF - 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase, at 608,298 to 609,173
lsrF
Position (kb) 605
606
607
608 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 604.954 kb on + strand, within gGDEF at 604.976 kb on + strand, within gGDEF at 605.055 kb on - strand, within gGDEF at 605.331 kb on + strand, within gGDEF at 605.331 kb on + strand, within gGDEF at 605.331 kb on + strand, within gGDEF at 605.331 kb on + strand, within gGDEF at 605.773 kb on + strand at 605.786 kb on - strand at 605.982 kb on + strand at 606.137 kb on - strand, within uxaB at 606.226 kb on + strand, within uxaB at 606.664 kb on + strand, within uxaB at 606.665 kb on - strand, within uxaB at 606.842 kb on + strand, within uxaB at 606.895 kb on + strand, within uxaB at 607.384 kb on + strand at 607.576 kb on - strand at 607.650 kb on + strand at 607.827 kb on + strand, within tam at 607.828 kb on - strand, within tam at 607.902 kb on + strand at 607.905 kb on - strand at 608.108 kb on + strand, within lsrG at 608.318 kb on - strand at 608.327 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 604,954 + gGDEF JDDGAC_03635 0.47 +0.7 604,976 + gGDEF JDDGAC_03635 0.49 -0.2 605,055 - gGDEF JDDGAC_03635 0.54 -1.6 605,331 + gGDEF JDDGAC_03635 0.74 -1.4 605,331 + gGDEF JDDGAC_03635 0.74 -2.5 605,331 + gGDEF JDDGAC_03635 0.74 +1.1 605,331 + gGDEF JDDGAC_03635 0.74 -0.3 605,773 + +0.3 605,786 - +1.3 605,982 + -2.5 606,137 - uxaB JDDGAC_03640 0.14 -1.0 606,226 + uxaB JDDGAC_03640 0.20 -0.1 606,664 + uxaB JDDGAC_03640 0.50 +1.4 606,665 - uxaB JDDGAC_03640 0.50 +4.2 606,842 + uxaB JDDGAC_03640 0.63 +1.0 606,895 + uxaB JDDGAC_03640 0.66 +2.4 607,384 + +0.7 607,576 - +1.2 607,650 + +0.5 607,827 + tam JDDGAC_03645 0.62 -1.2 607,828 - tam JDDGAC_03645 0.63 -0.2 607,902 + +1.4 607,905 - -2.4 608,108 + lsrG JDDGAC_03650 0.43 -0.5 608,318 - -2.1 608,327 - -1.2
Or see this region's nucleotide sequence