Strain Fitness in Escherichia coli ECRC98 around JDDGAC_18930

Experiment: WM_EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntescS and escT overlap by 1 nucleotidesescT and escU overlap by 8 nucleotidesescU and etgA overlap by 4 nucleotidesetgA and grlR are separated by 195 nucleotides JDDGAC_18920: escS - type III secretion system LEE export apparatus protein EscS, at 3,600,659 to 3,600,928 escS JDDGAC_18925: escT - type III secretion system LEE export apparatus protein EscT, at 3,600,928 to 3,601,704 escT JDDGAC_18930: escU - type III secretion system LEE export apparatus switch protein EscU, at 3,601,697 to 3,602,734 escU JDDGAC_18935: etgA - type III secretion system LEE muramidase EtgA, at 3,602,731 to 3,603,189 etgA JDDGAC_18940: grlR - type III secretion system LEE GrlA-binding negative regulator GrlR, at 3,603,385 to 3,603,756 grlR Position (kb) 3601 3602 3603Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3600.757 kb on + strand, within escSat 3600.779 kb on - strand, within escSat 3600.779 kb on - strand, within escSat 3600.789 kb on + strand, within escSat 3600.854 kb on + strand, within escSat 3601.290 kb on + strand, within escTat 3601.376 kb on + strand, within escTat 3602.002 kb on - strand, within escUat 3602.011 kb on - strand, within escUat 3602.449 kb on + strand, within escUat 3602.507 kb on + strand, within escUat 3602.769 kb on - strandat 3603.004 kb on - strand, within etgAat 3603.229 kb on + strandat 3603.407 kb on - strandat 3603.420 kb on + strandat 3603.537 kb on - strand, within grlRat 3603.553 kb on + strand, within grlRat 3603.556 kb on - strand, within grlR

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_EV240
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3,600,757 + escS JDDGAC_18920 0.36 +0.5
3,600,779 - escS JDDGAC_18920 0.44 +1.2
3,600,779 - escS JDDGAC_18920 0.44 +0.7
3,600,789 + escS JDDGAC_18920 0.48 +3.3
3,600,854 + escS JDDGAC_18920 0.72 +0.1
3,601,290 + escT JDDGAC_18925 0.47 -1.7
3,601,376 + escT JDDGAC_18925 0.58 -0.3
3,602,002 - escU JDDGAC_18930 0.29 -0.4
3,602,011 - escU JDDGAC_18930 0.30 -1.9
3,602,449 + escU JDDGAC_18930 0.72 +0.2
3,602,507 + escU JDDGAC_18930 0.78 +0.8
3,602,769 - +0.9
3,603,004 - etgA JDDGAC_18935 0.59 +1.5
3,603,229 + -0.6
3,603,407 - +0.3
3,603,420 + -0.5
3,603,537 - grlR JDDGAC_18940 0.41 +0.4
3,603,553 + grlR JDDGAC_18940 0.45 +3.8
3,603,556 - grlR JDDGAC_18940 0.46 -0.6

Or see this region's nucleotide sequence