Experiment: WM_TP12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt repA and putA1 are separated by 917 nucleotides putA1 and parE overlap by 1 nucleotides parE and JDDGAC_30210 are separated by 94 nucleotides JDDGAC_30210 and tra5 are separated by 358 nucleotides
JDDGAC_30195: repA - incFII family plasmid replication initiator RepA, at 59,252 to 60,109
repA
JDDGAC_30200: putA1 - CopG family transcriptional regulator, at 61,027 to 61,305
putA1
JDDGAC_30205: parE - plasmid stabilization protein, at 61,305 to 61,580
parE
JDDGAC_30210: JDDGAC_30210 - hypothetical protein, at 61,675 to 61,881
_30210
JDDGAC_30215: tra5 - IS3 family transposase, at 62,240 to 62,719
tra5
Position (kb) 61
62 Strain fitness (log2 ratio) -2
-1
0
1
2 at 60.218 kb on - strand at 60.394 kb on - strand at 60.441 kb on - strand at 60.470 kb on + strand at 60.470 kb on + strand at 60.470 kb on + strand at 60.471 kb on - strand at 60.592 kb on + strand at 60.707 kb on + strand at 60.718 kb on - strand at 60.749 kb on + strand at 60.750 kb on - strand at 60.800 kb on + strand at 60.827 kb on - strand at 60.904 kb on - strand at 60.914 kb on + strand at 60.915 kb on - strand at 60.920 kb on + strand at 60.929 kb on + strand at 60.939 kb on + strand at 60.939 kb on + strand at 60.939 kb on + strand at 60.939 kb on + strand at 60.940 kb on - strand at 60.940 kb on - strand at 60.940 kb on - strand at 60.940 kb on - strand at 60.995 kb on + strand at 61.084 kb on - strand, within putA1 at 61.084 kb on - strand, within putA1 at 61.250 kb on + strand, within putA1 at 61.351 kb on + strand, within parE at 61.351 kb on + strand, within parE at 61.352 kb on - strand, within parE at 61.353 kb on + strand, within parE at 61.354 kb on - strand, within parE at 61.400 kb on - strand, within parE at 61.432 kb on + strand, within parE at 61.446 kb on - strand, within parE at 61.459 kb on + strand, within parE at 61.526 kb on + strand, within parE at 61.539 kb on - strand, within parE at 61.539 kb on - strand, within parE at 61.543 kb on - strand, within parE at 61.547 kb on - strand, within parE at 61.578 kb on + strand at 61.579 kb on - strand at 61.600 kb on + strand at 61.600 kb on + strand at 61.600 kb on + strand at 61.601 kb on - strand at 61.601 kb on - strand at 61.601 kb on - strand at 61.601 kb on - strand at 61.601 kb on - strand at 61.605 kb on + strand at 61.636 kb on + strand at 61.637 kb on - strand at 61.665 kb on - strand at 61.665 kb on - strand at 61.741 kb on - strand, within JDDGAC_30210 at 61.743 kb on + strand, within JDDGAC_30210 at 61.744 kb on - strand, within JDDGAC_30210 at 61.753 kb on - strand, within JDDGAC_30210 at 61.753 kb on - strand, within JDDGAC_30210 at 61.753 kb on - strand, within JDDGAC_30210 at 61.830 kb on + strand, within JDDGAC_30210 at 61.830 kb on + strand, within JDDGAC_30210 at 61.832 kb on + strand, within JDDGAC_30210 at 61.832 kb on + strand, within JDDGAC_30210 at 61.832 kb on + strand, within JDDGAC_30210 at 61.832 kb on + strand, within JDDGAC_30210 at 61.832 kb on + strand, within JDDGAC_30210 at 61.832 kb on + strand, within JDDGAC_30210 at 61.832 kb on + strand, within JDDGAC_30210 at 61.832 kb on + strand, within JDDGAC_30210 at 61.832 kb on + strand, within JDDGAC_30210 at 61.833 kb on - strand, within JDDGAC_30210 at 61.833 kb on - strand, within JDDGAC_30210 at 61.833 kb on - strand, within JDDGAC_30210 at 61.833 kb on - strand, within JDDGAC_30210 at 61.833 kb on - strand, within JDDGAC_30210 at 61.833 kb on - strand, within JDDGAC_30210 at 61.837 kb on - strand, within JDDGAC_30210 at 61.837 kb on - strand, within JDDGAC_30210 at 61.837 kb on - strand, within JDDGAC_30210 at 61.839 kb on - strand, within JDDGAC_30210 at 61.842 kb on + strand, within JDDGAC_30210 at 61.864 kb on + strand at 62.000 kb on + strand at 62.001 kb on - strand at 62.001 kb on - strand at 62.205 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP12 remove 60,218 - +1.2 60,394 - +1.4 60,441 - -0.9 60,470 + -0.4 60,470 + +0.5 60,470 + +1.1 60,471 - -0.5 60,592 + +0.8 60,707 + +0.7 60,718 - +0.2 60,749 + +0.1 60,750 - +1.6 60,800 + +1.5 60,827 - -0.3 60,904 - -1.9 60,914 + +0.1 60,915 - +0.5 60,920 + -1.6 60,929 + +1.0 60,939 + -0.6 60,939 + +1.9 60,939 + +0.4 60,939 + -1.5 60,940 - +0.5 60,940 - +0.2 60,940 - -0.5 60,940 - +1.1 60,995 + +0.4 61,084 - putA1 JDDGAC_30200 0.20 -0.5 61,084 - putA1 JDDGAC_30200 0.20 -0.8 61,250 + putA1 JDDGAC_30200 0.80 -0.8 61,351 + parE JDDGAC_30205 0.17 +0.5 61,351 + parE JDDGAC_30205 0.17 -0.2 61,352 - parE JDDGAC_30205 0.17 -0.4 61,353 + parE JDDGAC_30205 0.17 -0.6 61,354 - parE JDDGAC_30205 0.18 +0.1 61,400 - parE JDDGAC_30205 0.34 -0.5 61,432 + parE JDDGAC_30205 0.46 +0.9 61,446 - parE JDDGAC_30205 0.51 -0.2 61,459 + parE JDDGAC_30205 0.56 +0.2 61,526 + parE JDDGAC_30205 0.80 -0.1 61,539 - parE JDDGAC_30205 0.85 -0.4 61,539 - parE JDDGAC_30205 0.85 +0.8 61,543 - parE JDDGAC_30205 0.86 +0.2 61,547 - parE JDDGAC_30205 0.88 +0.1 61,578 + +0.1 61,579 - +0.1 61,600 + +0.3 61,600 + -0.0 61,600 + +0.2 61,601 - -0.1 61,601 - -0.4 61,601 - +0.4 61,601 - -0.0 61,601 - -0.0 61,605 + +0.6 61,636 + +0.4 61,637 - +0.3 61,665 - -1.2 61,665 - -1.1 61,741 - JDDGAC_30210 0.32 +1.2 61,743 + JDDGAC_30210 0.33 +0.1 61,744 - JDDGAC_30210 0.33 -2.7 61,753 - JDDGAC_30210 0.38 -0.3 61,753 - JDDGAC_30210 0.38 +0.1 61,753 - JDDGAC_30210 0.38 +0.0 61,830 + JDDGAC_30210 0.75 -0.0 61,830 + JDDGAC_30210 0.75 +0.2 61,832 + JDDGAC_30210 0.76 -0.9 61,832 + JDDGAC_30210 0.76 -0.2 61,832 + JDDGAC_30210 0.76 -0.5 61,832 + JDDGAC_30210 0.76 +1.3 61,832 + JDDGAC_30210 0.76 +0.6 61,832 + JDDGAC_30210 0.76 +0.3 61,832 + JDDGAC_30210 0.76 -0.6 61,832 + JDDGAC_30210 0.76 -0.7 61,832 + JDDGAC_30210 0.76 -1.7 61,833 - JDDGAC_30210 0.76 +0.3 61,833 - JDDGAC_30210 0.76 +0.2 61,833 - JDDGAC_30210 0.76 +0.2 61,833 - JDDGAC_30210 0.76 -0.3 61,833 - JDDGAC_30210 0.76 -0.2 61,833 - JDDGAC_30210 0.76 -1.6 61,837 - JDDGAC_30210 0.78 +0.6 61,837 - JDDGAC_30210 0.78 -0.9 61,837 - JDDGAC_30210 0.78 -0.7 61,839 - JDDGAC_30210 0.79 -0.5 61,842 + JDDGAC_30210 0.81 +0.9 61,864 + -0.5 62,000 + +0.1 62,001 - -1.5 62,001 - -0.5 62,205 - -0.2
Or see this region's nucleotide sequence