Strain Fitness in Escherichia coli ECRC98 around JDDGAC_28560

Experiment: WM_TP12

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntJDDGAC_28550 and wcaG are separated by 2 nucleotideswcaG and wbdQ are separated by 2 nucleotideswbdQ and manC2 overlap by 19 nucleotides JDDGAC_28550: JDDGAC_28550 - GDP-mannose 4,6-dehydratase, at 5,478,374 to 5,479,492 _28550 JDDGAC_28555: wcaG - GDP-fucose synthetase, at 5,479,495 to 5,480,460 wcaG JDDGAC_28560: wbdQ - GDP-mannose mannosyl hydrolase WbdQ/WbhG, at 5,480,463 to 5,480,972 wbdQ JDDGAC_28565: manC2 - mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase, at 5,480,954 to 5,482,402 manC2 Position (kb) 5480 5481Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 5480.314 kb on - strand, within wcaGat 5480.470 kb on + strandat 5480.738 kb on + strand, within wbdQat 5480.768 kb on + strand, within wbdQat 5480.770 kb on + strand, within wbdQ

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP12
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5,480,314 - wcaG JDDGAC_28555 0.85 -1.3
5,480,470 + -4.4
5,480,738 + wbdQ JDDGAC_28560 0.54 -3.7
5,480,768 + wbdQ JDDGAC_28560 0.60 -1.3
5,480,770 + wbdQ JDDGAC_28560 0.60 -1.4

Or see this region's nucleotide sequence