Experiment: WM_TP12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyfF and hyfE are separated by 19 nucleotides hyfE and hyfD are separated by 11 nucleotides hyfD and hyfC are separated by 16 nucleotides hyfC and hyfB are separated by 10 nucleotides
JDDGAC_25820: hyfF - hydrogenase 4 subunit F, at 4,935,255 to 4,936,820
hyfF
JDDGAC_25825: hyfE - hydrogenase 4 membrane subunit, at 4,936,840 to 4,937,490
hyfE
JDDGAC_25830: hyfD - hydrogenase 4 subunit HyfD, at 4,937,502 to 4,938,941
hyfD
JDDGAC_25835: hyfC - hydrogenase 4 subunit HyfC, at 4,938,958 to 4,939,905
hyfC
JDDGAC_25840: hyfB - hydrogenase 4 subunit B, at 4,939,916 to 4,941,934
hyfB
Position (kb)
4937
4938
4939 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4936.547 kb on - strand, within hyfF at 4936.623 kb on - strand, within hyfF at 4936.714 kb on - strand at 4936.714 kb on - strand at 4936.718 kb on + strand at 4936.718 kb on + strand at 4936.718 kb on + strand at 4936.718 kb on + strand at 4936.718 kb on + strand at 4936.718 kb on + strand at 4936.719 kb on - strand at 4936.719 kb on - strand at 4936.722 kb on + strand at 4936.722 kb on + strand at 4936.722 kb on + strand at 4936.723 kb on - strand at 4936.723 kb on - strand at 4936.723 kb on - strand at 4936.723 kb on - strand at 4936.723 kb on - strand at 4936.725 kb on + strand at 4936.828 kb on + strand at 4937.258 kb on + strand, within hyfE at 4937.258 kb on + strand, within hyfE at 4937.258 kb on + strand, within hyfE at 4937.259 kb on - strand, within hyfE at 4937.259 kb on - strand, within hyfE at 4937.259 kb on - strand, within hyfE at 4937.467 kb on - strand at 4937.503 kb on + strand at 4937.504 kb on - strand at 4937.557 kb on - strand at 4937.652 kb on - strand, within hyfD at 4937.847 kb on + strand, within hyfD at 4937.888 kb on - strand, within hyfD at 4937.904 kb on + strand, within hyfD at 4937.947 kb on + strand, within hyfD at 4937.947 kb on + strand, within hyfD at 4937.948 kb on - strand, within hyfD at 4938.074 kb on - strand, within hyfD at 4938.074 kb on - strand, within hyfD at 4938.270 kb on + strand, within hyfD at 4938.403 kb on + strand, within hyfD at 4938.431 kb on + strand, within hyfD at 4938.432 kb on - strand, within hyfD at 4938.432 kb on - strand, within hyfD at 4938.445 kb on - strand, within hyfD at 4938.475 kb on + strand, within hyfD at 4938.478 kb on + strand, within hyfD at 4938.552 kb on + strand, within hyfD at 4938.576 kb on - strand, within hyfD at 4938.595 kb on + strand, within hyfD at 4938.596 kb on - strand, within hyfD at 4938.658 kb on + strand, within hyfD at 4938.708 kb on - strand, within hyfD at 4938.794 kb on - strand, within hyfD at 4938.902 kb on + strand at 4939.250 kb on + strand, within hyfC at 4939.273 kb on + strand, within hyfC at 4939.273 kb on + strand, within hyfC at 4939.395 kb on + strand, within hyfC at 4939.430 kb on - strand, within hyfC at 4939.578 kb on - strand, within hyfC at 4939.626 kb on + strand, within hyfC at 4939.626 kb on + strand, within hyfC at 4939.626 kb on + strand, within hyfC at 4939.709 kb on - strand, within hyfC at 4939.710 kb on + strand, within hyfC at 4939.710 kb on + strand, within hyfC at 4939.710 kb on + strand, within hyfC at 4939.731 kb on + strand, within hyfC at 4939.802 kb on - strand, within hyfC
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP12 remove 4,936,547 - hyfF JDDGAC_25820 0.83 +0.1 4,936,623 - hyfF JDDGAC_25820 0.87 +0.2 4,936,714 - -0.1 4,936,714 - -1.6 4,936,718 + +0.5 4,936,718 + +0.8 4,936,718 + +0.7 4,936,718 + -2.2 4,936,718 + +1.6 4,936,718 + +0.3 4,936,719 - -0.1 4,936,719 - -0.1 4,936,722 + -0.2 4,936,722 + +0.4 4,936,722 + +0.0 4,936,723 - -0.6 4,936,723 - +0.9 4,936,723 - -0.7 4,936,723 - +0.6 4,936,723 - -0.7 4,936,725 + +0.7 4,936,828 + +0.5 4,937,258 + hyfE JDDGAC_25825 0.64 +0.5 4,937,258 + hyfE JDDGAC_25825 0.64 -0.1 4,937,258 + hyfE JDDGAC_25825 0.64 +1.0 4,937,259 - hyfE JDDGAC_25825 0.64 -1.6 4,937,259 - hyfE JDDGAC_25825 0.64 -0.4 4,937,259 - hyfE JDDGAC_25825 0.64 +0.5 4,937,467 - +0.7 4,937,503 + -0.1 4,937,504 - +0.5 4,937,557 - -0.3 4,937,652 - hyfD JDDGAC_25830 0.10 +0.9 4,937,847 + hyfD JDDGAC_25830 0.24 -0.3 4,937,888 - hyfD JDDGAC_25830 0.27 +3.5 4,937,904 + hyfD JDDGAC_25830 0.28 +0.6 4,937,947 + hyfD JDDGAC_25830 0.31 -0.5 4,937,947 + hyfD JDDGAC_25830 0.31 +0.3 4,937,948 - hyfD JDDGAC_25830 0.31 -1.0 4,938,074 - hyfD JDDGAC_25830 0.40 +0.6 4,938,074 - hyfD JDDGAC_25830 0.40 -2.0 4,938,270 + hyfD JDDGAC_25830 0.53 -1.8 4,938,403 + hyfD JDDGAC_25830 0.63 +0.7 4,938,431 + hyfD JDDGAC_25830 0.65 +0.0 4,938,432 - hyfD JDDGAC_25830 0.65 +0.3 4,938,432 - hyfD JDDGAC_25830 0.65 +0.0 4,938,445 - hyfD JDDGAC_25830 0.65 -1.0 4,938,475 + hyfD JDDGAC_25830 0.68 +2.0 4,938,478 + hyfD JDDGAC_25830 0.68 +1.2 4,938,552 + hyfD JDDGAC_25830 0.73 +0.2 4,938,576 - hyfD JDDGAC_25830 0.75 +1.6 4,938,595 + hyfD JDDGAC_25830 0.76 +0.6 4,938,596 - hyfD JDDGAC_25830 0.76 -0.7 4,938,658 + hyfD JDDGAC_25830 0.80 -0.1 4,938,708 - hyfD JDDGAC_25830 0.84 -0.7 4,938,794 - hyfD JDDGAC_25830 0.90 -0.3 4,938,902 + -1.5 4,939,250 + hyfC JDDGAC_25835 0.31 -0.8 4,939,273 + hyfC JDDGAC_25835 0.33 -2.8 4,939,273 + hyfC JDDGAC_25835 0.33 -0.3 4,939,395 + hyfC JDDGAC_25835 0.46 +0.1 4,939,430 - hyfC JDDGAC_25835 0.50 +0.0 4,939,578 - hyfC JDDGAC_25835 0.65 -0.2 4,939,626 + hyfC JDDGAC_25835 0.70 -1.6 4,939,626 + hyfC JDDGAC_25835 0.70 -0.3 4,939,626 + hyfC JDDGAC_25835 0.70 -0.2 4,939,709 - hyfC JDDGAC_25835 0.79 +0.2 4,939,710 + hyfC JDDGAC_25835 0.79 +0.7 4,939,710 + hyfC JDDGAC_25835 0.79 +0.2 4,939,710 + hyfC JDDGAC_25835 0.79 -0.6 4,939,731 + hyfC JDDGAC_25835 0.82 -0.1 4,939,802 - hyfC JDDGAC_25835 0.89 -0.3
Or see this region's nucleotide sequence