Experiment: WM_TP12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lgt and thyA are separated by 6 nucleotides thyA and ppdA are separated by 183 nucleotides ppdA and pulJ overlap by 10 nucleotides pulJ and ygdB overlap by 4 nucleotides ygdB and ppdC overlap by 16 nucleotides
JDDGAC_23675: lgt - prolipoprotein diacylglyceryl transferase, at 4,531,992 to 4,532,867
lgt
JDDGAC_23680: thyA - thymidylate synthase, at 4,532,874 to 4,533,668
thyA
JDDGAC_23685: ppdA - prepilin peptidase-dependent protein, at 4,533,852 to 4,534,322
ppdA
JDDGAC_23690: pulJ - prepilin peptidase-dependent protein, at 4,534,313 to 4,534,876
pulJ
JDDGAC_23695: ygdB - DUF2509 domain-containing protein, at 4,534,873 to 4,535,280
ygdB
JDDGAC_23700: ppdC - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 4,535,265 to 4,535,588
ppdC
Position (kb) 4533
4534
4535 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4533.667 kb on - strand at 4533.667 kb on - strand at 4533.667 kb on - strand at 4533.776 kb on + strand at 4533.872 kb on + strand at 4533.872 kb on + strand at 4533.873 kb on - strand at 4533.873 kb on - strand at 4533.916 kb on + strand, within ppdA at 4533.917 kb on - strand, within ppdA at 4533.934 kb on - strand, within ppdA at 4533.934 kb on - strand, within ppdA at 4533.934 kb on - strand, within ppdA at 4533.997 kb on - strand, within ppdA at 4533.997 kb on - strand, within ppdA at 4534.009 kb on + strand, within ppdA at 4534.073 kb on - strand, within ppdA at 4534.321 kb on - strand at 4534.476 kb on + strand, within pulJ at 4534.476 kb on + strand, within pulJ at 4534.480 kb on + strand, within pulJ at 4534.480 kb on + strand, within pulJ at 4534.512 kb on - strand, within pulJ at 4534.567 kb on + strand, within pulJ at 4534.567 kb on + strand, within pulJ at 4534.567 kb on + strand, within pulJ at 4534.567 kb on + strand, within pulJ at 4534.567 kb on + strand, within pulJ at 4534.568 kb on - strand, within pulJ at 4534.699 kb on + strand, within pulJ at 4534.798 kb on + strand, within pulJ at 4534.799 kb on - strand, within pulJ at 4534.801 kb on + strand, within pulJ at 4534.801 kb on + strand, within pulJ at 4534.802 kb on - strand, within pulJ at 4534.816 kb on + strand, within pulJ at 4534.941 kb on - strand, within ygdB at 4534.941 kb on - strand, within ygdB at 4534.944 kb on - strand, within ygdB at 4535.087 kb on + strand, within ygdB at 4535.092 kb on + strand, within ygdB at 4535.092 kb on + strand, within ygdB at 4535.150 kb on + strand, within ygdB at 4535.150 kb on + strand, within ygdB at 4535.150 kb on + strand, within ygdB at 4535.150 kb on + strand, within ygdB at 4535.151 kb on - strand, within ygdB at 4535.161 kb on + strand, within ygdB at 4535.161 kb on + strand, within ygdB
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP12 remove 4,533,667 - +1.2 4,533,667 - +0.4 4,533,667 - -0.3 4,533,776 + +0.5 4,533,872 + -0.4 4,533,872 + +1.5 4,533,873 - +0.7 4,533,873 - -0.4 4,533,916 + ppdA JDDGAC_23685 0.14 +0.5 4,533,917 - ppdA JDDGAC_23685 0.14 +0.2 4,533,934 - ppdA JDDGAC_23685 0.17 -2.6 4,533,934 - ppdA JDDGAC_23685 0.17 +0.5 4,533,934 - ppdA JDDGAC_23685 0.17 +0.4 4,533,997 - ppdA JDDGAC_23685 0.31 +0.4 4,533,997 - ppdA JDDGAC_23685 0.31 -0.1 4,534,009 + ppdA JDDGAC_23685 0.33 +0.3 4,534,073 - ppdA JDDGAC_23685 0.47 -0.2 4,534,321 - -0.0 4,534,476 + pulJ JDDGAC_23690 0.29 -0.2 4,534,476 + pulJ JDDGAC_23690 0.29 +0.6 4,534,480 + pulJ JDDGAC_23690 0.30 +1.0 4,534,480 + pulJ JDDGAC_23690 0.30 -0.4 4,534,512 - pulJ JDDGAC_23690 0.35 -1.9 4,534,567 + pulJ JDDGAC_23690 0.45 -0.0 4,534,567 + pulJ JDDGAC_23690 0.45 +0.3 4,534,567 + pulJ JDDGAC_23690 0.45 +0.5 4,534,567 + pulJ JDDGAC_23690 0.45 +0.3 4,534,567 + pulJ JDDGAC_23690 0.45 +1.5 4,534,568 - pulJ JDDGAC_23690 0.45 -0.7 4,534,699 + pulJ JDDGAC_23690 0.68 +0.1 4,534,798 + pulJ JDDGAC_23690 0.86 +0.3 4,534,799 - pulJ JDDGAC_23690 0.86 -2.9 4,534,801 + pulJ JDDGAC_23690 0.87 +2.0 4,534,801 + pulJ JDDGAC_23690 0.87 +0.3 4,534,802 - pulJ JDDGAC_23690 0.87 -0.3 4,534,816 + pulJ JDDGAC_23690 0.89 +0.0 4,534,941 - ygdB JDDGAC_23695 0.17 +1.1 4,534,941 - ygdB JDDGAC_23695 0.17 +0.6 4,534,944 - ygdB JDDGAC_23695 0.17 +2.1 4,535,087 + ygdB JDDGAC_23695 0.52 +0.3 4,535,092 + ygdB JDDGAC_23695 0.54 +0.5 4,535,092 + ygdB JDDGAC_23695 0.54 +0.8 4,535,150 + ygdB JDDGAC_23695 0.68 -0.1 4,535,150 + ygdB JDDGAC_23695 0.68 -0.1 4,535,150 + ygdB JDDGAC_23695 0.68 -1.6 4,535,150 + ygdB JDDGAC_23695 0.68 +0.1 4,535,151 - ygdB JDDGAC_23695 0.68 +1.2 4,535,161 + ygdB JDDGAC_23695 0.71 -0.2 4,535,161 + ygdB JDDGAC_23695 0.71 +0.5
Or see this region's nucleotide sequence