Experiment: WM_TP12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tsaD and ttdT are separated by 42 nucleotides ttdT and ttdB are separated by 48 nucleotides ttdB and ttdA overlap by 4 nucleotides
JDDGAC_22305: tsaD - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD, at 4,267,897 to 4,268,910
tsaD
JDDGAC_22310: ttdT - L-tartrate/succinate antiporter, at 4,268,953 to 4,270,416
ttdT
JDDGAC_22315: ttdB - L(+)-tartrate dehydratase subunit beta, at 4,270,465 to 4,271,070
ttdB
JDDGAC_22320: ttdA - L(+)-tartrate dehydratase subunit alpha, at 4,271,067 to 4,271,978
ttdA
Position (kb)
4268
4269
4270
4271 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4268.909 kb on - strand at 4268.914 kb on + strand at 4268.915 kb on - strand at 4268.932 kb on + strand at 4268.932 kb on + strand at 4268.951 kb on - strand at 4268.954 kb on + strand at 4268.980 kb on + strand at 4268.980 kb on + strand at 4268.980 kb on + strand at 4268.980 kb on + strand at 4268.981 kb on - strand at 4269.001 kb on + strand at 4269.002 kb on - strand at 4269.010 kb on + strand at 4269.010 kb on + strand at 4269.011 kb on - strand at 4269.060 kb on - strand at 4269.072 kb on - strand at 4269.098 kb on + strand at 4269.106 kb on - strand, within ttdT at 4269.106 kb on - strand, within ttdT at 4269.216 kb on + strand, within ttdT at 4269.218 kb on + strand, within ttdT at 4269.218 kb on + strand, within ttdT at 4269.218 kb on + strand, within ttdT at 4269.218 kb on + strand, within ttdT at 4269.219 kb on - strand, within ttdT at 4269.243 kb on - strand, within ttdT at 4269.251 kb on - strand, within ttdT at 4269.251 kb on - strand, within ttdT at 4269.255 kb on - strand, within ttdT at 4269.304 kb on + strand, within ttdT at 4269.317 kb on - strand, within ttdT at 4269.336 kb on + strand, within ttdT at 4269.336 kb on + strand, within ttdT at 4269.415 kb on - strand, within ttdT at 4269.418 kb on - strand, within ttdT at 4269.427 kb on - strand, within ttdT at 4269.427 kb on - strand, within ttdT at 4269.498 kb on + strand, within ttdT at 4269.500 kb on + strand, within ttdT at 4269.501 kb on - strand, within ttdT at 4269.501 kb on - strand, within ttdT at 4269.635 kb on - strand, within ttdT at 4269.654 kb on - strand, within ttdT at 4269.774 kb on + strand, within ttdT at 4269.775 kb on - strand, within ttdT at 4269.843 kb on - strand, within ttdT at 4269.843 kb on - strand, within ttdT at 4269.843 kb on - strand, within ttdT at 4269.843 kb on - strand, within ttdT at 4269.843 kb on - strand, within ttdT at 4269.843 kb on - strand, within ttdT at 4269.887 kb on + strand, within ttdT at 4269.888 kb on - strand, within ttdT at 4269.917 kb on + strand, within ttdT at 4269.921 kb on + strand, within ttdT at 4269.922 kb on - strand, within ttdT at 4269.993 kb on - strand, within ttdT at 4270.086 kb on + strand, within ttdT at 4270.111 kb on - strand, within ttdT at 4270.216 kb on + strand, within ttdT at 4270.216 kb on + strand, within ttdT at 4270.217 kb on - strand, within ttdT at 4270.217 kb on - strand, within ttdT at 4270.234 kb on - strand, within ttdT at 4270.310 kb on + strand at 4270.320 kb on + strand at 4270.320 kb on + strand at 4270.321 kb on - strand at 4270.321 kb on - strand at 4270.321 kb on - strand at 4270.321 kb on - strand at 4270.351 kb on + strand at 4270.352 kb on - strand at 4270.389 kb on - strand at 4270.422 kb on + strand at 4270.422 kb on + strand at 4270.423 kb on - strand at 4270.467 kb on - strand at 4270.475 kb on + strand at 4270.476 kb on - strand at 4270.476 kb on - strand at 4270.476 kb on - strand at 4270.573 kb on - strand, within ttdB at 4270.708 kb on + strand, within ttdB at 4270.709 kb on - strand, within ttdB at 4270.715 kb on - strand, within ttdB at 4270.825 kb on - strand, within ttdB at 4270.831 kb on - strand, within ttdB at 4270.926 kb on - strand, within ttdB at 4271.001 kb on - strand, within ttdB at 4271.001 kb on - strand, within ttdB at 4271.001 kb on - strand, within ttdB at 4271.022 kb on + strand at 4271.023 kb on - strand at 4271.284 kb on - strand, within ttdA at 4271.355 kb on + strand, within ttdA at 4271.400 kb on + strand, within ttdA
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP12 remove 4,268,909 - -0.6 4,268,914 + -0.1 4,268,915 - -0.5 4,268,932 + +1.1 4,268,932 + +0.7 4,268,951 - -0.4 4,268,954 + +0.6 4,268,980 + -0.2 4,268,980 + +0.7 4,268,980 + -0.3 4,268,980 + +0.3 4,268,981 - -0.8 4,269,001 + -0.1 4,269,002 - -1.1 4,269,010 + +0.1 4,269,010 + +0.3 4,269,011 - -1.6 4,269,060 - -0.2 4,269,072 - -1.1 4,269,098 + -0.2 4,269,106 - ttdT JDDGAC_22310 0.10 -0.3 4,269,106 - ttdT JDDGAC_22310 0.10 +0.3 4,269,216 + ttdT JDDGAC_22310 0.18 +0.2 4,269,218 + ttdT JDDGAC_22310 0.18 -3.3 4,269,218 + ttdT JDDGAC_22310 0.18 -0.3 4,269,218 + ttdT JDDGAC_22310 0.18 -1.0 4,269,218 + ttdT JDDGAC_22310 0.18 +0.3 4,269,219 - ttdT JDDGAC_22310 0.18 +0.8 4,269,243 - ttdT JDDGAC_22310 0.20 -1.0 4,269,251 - ttdT JDDGAC_22310 0.20 -0.1 4,269,251 - ttdT JDDGAC_22310 0.20 +0.1 4,269,255 - ttdT JDDGAC_22310 0.21 -0.9 4,269,304 + ttdT JDDGAC_22310 0.24 +0.8 4,269,317 - ttdT JDDGAC_22310 0.25 +1.2 4,269,336 + ttdT JDDGAC_22310 0.26 +0.1 4,269,336 + ttdT JDDGAC_22310 0.26 -0.2 4,269,415 - ttdT JDDGAC_22310 0.32 -0.7 4,269,418 - ttdT JDDGAC_22310 0.32 +0.5 4,269,427 - ttdT JDDGAC_22310 0.32 +0.1 4,269,427 - ttdT JDDGAC_22310 0.32 -0.8 4,269,498 + ttdT JDDGAC_22310 0.37 -0.3 4,269,500 + ttdT JDDGAC_22310 0.37 +0.2 4,269,501 - ttdT JDDGAC_22310 0.37 +0.7 4,269,501 - ttdT JDDGAC_22310 0.37 -1.7 4,269,635 - ttdT JDDGAC_22310 0.47 -1.2 4,269,654 - ttdT JDDGAC_22310 0.48 -0.5 4,269,774 + ttdT JDDGAC_22310 0.56 +0.2 4,269,775 - ttdT JDDGAC_22310 0.56 -1.1 4,269,843 - ttdT JDDGAC_22310 0.61 -1.1 4,269,843 - ttdT JDDGAC_22310 0.61 -0.6 4,269,843 - ttdT JDDGAC_22310 0.61 +0.1 4,269,843 - ttdT JDDGAC_22310 0.61 +0.3 4,269,843 - ttdT JDDGAC_22310 0.61 -0.0 4,269,843 - ttdT JDDGAC_22310 0.61 +0.3 4,269,887 + ttdT JDDGAC_22310 0.64 -0.3 4,269,888 - ttdT JDDGAC_22310 0.64 +0.4 4,269,917 + ttdT JDDGAC_22310 0.66 -0.5 4,269,921 + ttdT JDDGAC_22310 0.66 -0.4 4,269,922 - ttdT JDDGAC_22310 0.66 -0.3 4,269,993 - ttdT JDDGAC_22310 0.71 +0.3 4,270,086 + ttdT JDDGAC_22310 0.77 +1.1 4,270,111 - ttdT JDDGAC_22310 0.79 +0.2 4,270,216 + ttdT JDDGAC_22310 0.86 -0.1 4,270,216 + ttdT JDDGAC_22310 0.86 -0.3 4,270,217 - ttdT JDDGAC_22310 0.86 -0.1 4,270,217 - ttdT JDDGAC_22310 0.86 +0.2 4,270,234 - ttdT JDDGAC_22310 0.88 +0.1 4,270,310 + -0.0 4,270,320 + +0.1 4,270,320 + -1.8 4,270,321 - +0.0 4,270,321 - +0.8 4,270,321 - -0.0 4,270,321 - -1.1 4,270,351 + -1.5 4,270,352 - -0.1 4,270,389 - +0.4 4,270,422 + -0.3 4,270,422 + +0.0 4,270,423 - +0.8 4,270,467 - +1.1 4,270,475 + -0.5 4,270,476 - -0.3 4,270,476 - +0.4 4,270,476 - -0.5 4,270,573 - ttdB JDDGAC_22315 0.18 -0.9 4,270,708 + ttdB JDDGAC_22315 0.40 +0.3 4,270,709 - ttdB JDDGAC_22315 0.40 +0.4 4,270,715 - ttdB JDDGAC_22315 0.41 +0.2 4,270,825 - ttdB JDDGAC_22315 0.59 +0.1 4,270,831 - ttdB JDDGAC_22315 0.60 +0.1 4,270,926 - ttdB JDDGAC_22315 0.76 +0.6 4,271,001 - ttdB JDDGAC_22315 0.88 -1.2 4,271,001 - ttdB JDDGAC_22315 0.88 -0.8 4,271,001 - ttdB JDDGAC_22315 0.88 -0.1 4,271,022 + +1.3 4,271,023 - -3.1 4,271,284 - ttdA JDDGAC_22320 0.24 +0.1 4,271,355 + ttdA JDDGAC_22320 0.32 -0.2 4,271,400 + ttdA JDDGAC_22320 0.37 +0.8
Or see this region's nucleotide sequence